Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 61 for “"Pore Formation"”.
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Templated self-assembly of nanoporous alumina : pore formation and ordering mechanisms, methodologies, and applications
… and biological applications due to its small pore size (4-200nm) and spacing (10-500nm). Under slightly acidic conditions, both oxidation and dissolution of aluminum leads to the formation of pores. AAO pores form a self-assembled honey-comb structure with short range order over certain ranges …
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<em>Agrobacterium</em> Virb10 Contributions to Type Iv Substrate Secretion, T-Pilus Biogenesis, and Outer Membrane Pore Formation
… of VirB10 to substrate transfer and T-pilus formation through a mutational analysis. VirB10 is a bitopic protein with several domains, including a: (i) cytoplasmic N-terminus, (ii) single transmembrane (TM) α-helix, (iii) proline-rich region (PRR), and (iv) large C-terminal modified β-barrel. …
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Computational investigations into two biological processes: membrane reshaping by light-harvesting proteins in the chromatophores of purple bacteria, and oligomerization and pore formation by the Hepatitis C viroporin p7
… p7, which oligomerizes to form ion-conducting pores. The presence of functional p7 has been shown to be critical for the late stages of the viral lifecycle, making it a potential drug target. This dissertation explores several possibilities for the structure of the p7 oligomer, finding that p7 …
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Staphylococcus aureus Panton-Valentine leukocidin (PVL) alters human neutrophil viability and function
… pathogenesis--if any--remains unknown. PVL is a pore-forming toxin with specificity for myeloid cells, including polymorphonuclear leukocytes (PMNs or neutrophils). It is widely assumed that PVL-mediated pore formation in the plasma membrane of PMNs leads ultimately to cytolysis. However, this …
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Staphylococcus aureus Panton-Valentine leukocidin (PVL) alters human neutrophil viability and function
… pathogenesis--if any--remains unknown. PVL is a pore-forming toxin with specificity for myeloid cells, including polymorphonuclear leukocytes (PMNs or neutrophils). It is widely assumed that PVL-mediated pore formation in the plasma membrane of PMNs leads ultimately to cytolysis. However, this …
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Mathematical modeling of microshrinkage formation during solidification of A356 alloy castings
… (and interacting) phenomena responsible for its formation and growth. Shrinkage porosity occurs by nucleation and growth phenomena. Very few previous models for shrinkage prediction consider the pore formation based on a nucleation and growth basis. Several models that can predict micro shrinkage …
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Interactions of functionalized vesicles in the presence of Europium (III) Chloride
… injections of micromolar EuCl¬3 induced membrane pore formation and membrane fusion. Our collection of results leads to the conclusion that formation of intra-molecular ligand receptor complexes leads to pore formation and inter-membrane complex formation leads to membrane fusion. Detailed …
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Porosity in Nitride Semiconductors
… etching (ECE) can be used to create pores in nitride semiconductors in order to engineer their properties. ECE offers a simple, flexible approach to pore formation, driven by an electric field between the material surface and a suitable electrolyte. This means that the material must …
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Controlled Intracellular delivery of molecules using Nanoparticle-mediated Photoporation
… delivery through transient cell membrane pore formation. In this thesis, we have explored the effects of changes in cellular microenvironment on cellular responses. Specifically, we highlight the role of nanoparticle and associated transient parameters, investigate cell responses to the …
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Interactions of Proteins with Membranes: Insights from Molecular Dynamics Simulations
… microscopy often struggle to capture the full conformational landscape of membrane proteins due to their complex interactions with lipid bilayers and their dynamic nature. These limitations make computational approaches, particularly molecular dynamics (MD) simulations, invaluable tools for …
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Entamoeba histolytica-induced Activation of Caspase-4 Regulates Gasdermin D Cleavage to Mediate IL-1β Secretion in Macrophages
… domain into the cell membrane, driving both pore formation and IL-1β secretion without causing significant cell death. In this study, I interrogated the requirements and molecular mechanism for Eh-induced caspase-4 activation in cleaving GSDMD and in initiating a robust pro-inflammatory …
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Synthesis and solid state F-NMR studies on fluorine-labeled antimicrobial peptide RK-21
… results support a mechanism of toroidal pore formation. iv</p>
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Reconfiguring the side-chain functionality of cationic helical polypeptides toward maximized gene delivery capabilities
… to their amine-containing analogues via the pore formation mechanism, and thus possessed notably higher transfection efficiencies. Elongating the hydrophobic side chain also potentiated the membrane activities of the polypeptides, while at the meantime caused higher cytotoxicities. Upon an …
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Characterization Of Streptolysin O As The Translocator For The Cytolysin-Mediated Translocation Process In Streptococcus Pyogenes
… <italic>Streptococcus pyogenes</italic> uses the pore-forming protein <underline>s</underline>trepto<underline>l</underline>ysin <underline>O</underline>: SLO) to translocate the <italic><underline>S.</underline></italic> <italic><underline>p</underline>yogenes</italic> …
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A study of the effect of anaesthetic molecules on model membranes formed in surfactant / water mixtures
… point of this project was to propose that pore formation might be a possible explanation for the anaesthetic action upon model membranes. The study focused on three model membrane systems and used techniques such as NMR and SAXS to obtain information about any structural changes that may …
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Deciphering the regulation of perforin-2-mediated endocytic escape in dendritic cells
… to my project, the Kozik lab had identified the pore-forming protein perforin-2 as a dedicated effector of endocytic escape that allows cytosolic entry of exogenous antigen during cross-presentation. Perforin-2 is a member of the membrane attack complex and perforin superfamily (MACPF) and, …
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Identifizierung regulatorischer Proteindomänen der zytolytischen Eigenschaften des humanen P2X7-Rezeptors
… to build both an ion channel and a cytolytic-pore which leads to membrane-blebbing. The aim of this MD-thesis was to develop a method which provides evidence whether the activation of selected P2X7-receptor-mutants with agonists like ATP or the more potent BzATP mediates a cytolytic membrane …
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Surface tension and electroporation of lipid bilayers
… be effectively used and developed. From aqueous pore formation theory, electroporation behavior is known to be largely dictated by surface energy. We hypothesize that this surface energy can be described by separate head and tail components of the lipid molecules, which can be obtained …
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