Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"Polyubiquitination"”.
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Key Role of Lys63-Linked Polyubiquitination in Viral Activation of IRF3
… were obtained indicating that Lys63-linked polyubiquitination played a key role in MAVS-mediated IRF3 activation both in vitro and in vivo. Finally, NEMO was demonstrated to function as a ubiquitin-chain adaptor recruiting and activating TBK1, the kinase for IRF3 phosphorylation. Those …
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Non-proteolytic roles of the ubiquitin-proteasome system in memory formation across the lifespan
… functions, such as lysine-63 (K63) polyubiquitination. Here, we investigated the role of histone H2Aubi in memory formation and K63 polyubiquitination in age-related memory decline, focusing on the hippocampus and amygdala—two regions critical for memory processing. We observed …
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Biochemical Mechanism of Protein Kinase Activation by the Ubiquitination System
… TAK1-IKK cascade by TRAF6 and Ubc13/Uev1A in a polyubiquitinationdependent manner. Mechanistically how TAK1 is activated by the TRAF6- Ubc13/Uev1A dependent polyubiquitination system is unknown. Whether TAK1 and IKK kinases can be activated by other than TRAF6-Ubc13/Uev1A is an open question. By …
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Novel Posttranslational Modification In Lkb1 Activation and Function
… unclear. Here we show that K63-linked LKB1 polyubiquitination by the Skp1-Cul1-F-box-protein/Skp2 (Skp2-SCF) ubiquitin ligase complex is critical for LKB1 activation by a mechanism of maintaining the LKB1-STRAD-MO25 complex integrity. We further demonstrate that oncogenic Ras acts upstream …
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Evaluation of Myogenic Differentiation Potential of BMD iPSCs in vitro and the Effect of Ubiquitination Inhibitors on Dystrophin Stability in vivo
… of dystrophin stability by repressing polyubiquitination is a promising strategy. The application of human iPSCs in tissue regeneration also serves as a potential therapeutic strategy. This study aims to test the efficacy of selected drugs interfering with the dystrophin …
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Sex-differences in proteasome-independent roles of the ubiquitin proteasome system in memory formation
… can gain one (monoubiquitination) or multiple (polyubiquitination) ubiquitins. The 26S proteasome is the catalytic component of the UPS and is comprised of a 20S catalytic core surrounded by two 19S regulatory caps. Phosphorylation of 19S cap regulatory subunit RPT6 at serine 120 (pRPT6-S120) …
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ANALYSIS OF ELECTROPHILE-INDUCED NRF2 GENE ACTIVATION
… by immunoblotting and LC-MS-MS. Keap1 polyubiquitination coincided with Nrf2 stabilization and nuclear accumulation. In contrast, BMCC did not induce Keap1 polyubiquitination. Our results suggest that Nrf2 activation is regulated through the polyubiquitination of Keap1, which in turn is …
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STRUCTURAL INSIGHTS INTO POLYUBIQUITIN CHAIN FORMATION MEDIATED BY NEDD4 HECT LIGASE
… and mechanistic aspects of NEDD4-mediated polyubiquitination remain enigmatic. Our lab previously identified a ubiquitin exosite within HECT as critical determinant for enzyme processivity. Here, we unveil that the ubiquitin exosite does not act as allosteric activator but likely stabilizes …
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Phopsphorylation and Ubiquitin Modification At Dna Damage Sites In Response to Double-Strand Breaks
… (IR)-induced DNA damage catalyzes Lys63-linked polyubiquitination of histones, H2A and H2A.X that leads to accumulation of BRCA1- A complex to DSBs. In my second study, we sought to determine whether non- lys63-linked ubiquitination also exists at the DSBs regulating the DDR pathway. My findings …
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New insights into a complex host-pathogen interaction: mechanisms of Helicobacter pylori activation of NF-κB
… and JNK signaling pathways, and enhances the polyubiquitination and iii activation of the kinase. This lysine 63-linked ubiquitination is mediated by E3 ligase tumor necrosis factor receptor-associated factor 6 (TRAF6) and E2 Ubc13. These findings show that polyubiquitination of TAK1 regulates …
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Age related memory loss and the role of the ubiquitin proteasome system
… memory decline. In terms of M1/linear polyubiquitination in aging, there was an increase in the aged hippocampus in linear polyubiquitin levels at rest but reduced levels of linear polyubiquitin in response to learning. CRISPR-dCas9 manipulation of Rnf31 levels to increase linear …
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A study on PIP5Kalpha degradation by Nedd4-1-mediated ubiquitination
… proteasomal degradation via Nedd4-1-mediated polyubiquitination at lysine 88, which leads to reduction in PIP2 levels. Furthermore, we found that the mutation K88R enhances the inhibition of neurite outgrowth in Neuro2A cells. Our results suggest a functional link of Nedd4 family to modulation …
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Nprl2/Tusc4 Functions As A Tumor Suppressor By Regulating Brca1’S Stability Via The E3 Ubiquitination Pathway
… of NPRL2/TUSC4 expression enhanced BRCA1 polyubiquitination, leading to BRCA1 protein degradation and a marked reduction in HR repair efficiency. Conversely, ectopic expression of NPRL2/TUSC4 effectively suppressed the proliferation, invasion, and colony formation of breast cancer cells …
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A Host Regulatory Circuit of HSV Gene Expression
… TBK1 and TRIM23. This complex targets ICP0 for polyubiquitination and subsequent proteasomal degradation. Therefore, in addition to inducing IFN and promoting autophagy, STING directly regulates HSV immediate-early gene expression. These data indicate that a previously unrecognized regulatory …
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Mechanistic Studies of the Activation of Ubiquitin-Conjugating Enzymes by Ring-Type Ubiquitin Ligases
… that thioester release measurements agree with polyubiquitination of physiological targets. We have structurally characterized our model E2 enzyme UbcH5b, it mutants, and its interaction with E3 enzymes. Taken together, our data strongly demonstrate that E3 enzymes are, apart from their …
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Analysis of post-translational modifications of the blue light photoreceptor, phot1, by the CRL3NPH3 ubiquitin ligase complex
… complex is necessary for the mono/multi- and polyubiquitination of phot1 under high fluence rate conditions in addition to the mono/multiubiquitination of phot1 under low fluence rate conditions. Preliminary evidence suggested that mono/multiubiquitination of phot1 is necessary for BL …
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Defining the Physiological and Molecular Role of Pellino3 in TLR3 Signalling
… in turn interacts with TRAF6 and facilitates its polyubiquitination. This modification of TRAF6 suppresses its ability to bind and ubiquitinate a key anti-viral transcription factor IRF7, thereby down regulating the type 1 IFN response. The findings outlined in this thesis define for the first …
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Decoding Lysine-11 Signals in Ubiquitination
… distinct roles for homotypic and heterotypic K11-polyubiquitination in proteasome-mediated degradation.
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An investigation of the role of TRIB2 in steady state and stressed haematopoiesis
… CDC25A isoform. Overexpression of TRIB2 promotes polyubiquitination and degradation of CDC25C. Hence, future works are warranted to examine TRIB2-CDC25C interaction in the context of developing thymocytes and in T-cell acute lymphoblastic leukaemia, the malignant counterpart.
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