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Showing 1 to 20 of 24 for “"Polytene"”.
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Localization of Small-Nuclear-Rna Genes of Drosophila Melanogaster on Polytene Chromosomes
The existence of discrete, stable, small nuclear RNAs (snRNAs) is known since 1960s. These RNAs range in size from 90-300 nucleotides and are confined to eukaryotic nuclei. snRNAs have been well-characterized biochemically and have been isolated and sequenced from different organisms. Recent …
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Pressure Treatment of Polytene Chromosomes and Computer Vision Techniques for High Resolution Cytogenetic Studies
… by the interphase chromatin organization in polytene chromosomes has made Drosophila the premier model organism for analysis of chromosome structure and behavior. A major impediment to full exploitation of polytene chromosome cytology has been the difficulty in producing high quality …
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Transcription Rates of Polytene Chromosome Bands and Their Relation to Gene Expression: An Autoradiographic Study With Drosophila Melanogaster
Made available in DSpace on 2015-05-14T14:56:45Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 7206955.PDF: 5067821 bytes, checksum: 3955a6bc5bbe32c2421c32ce0323d63a (MD5) Previous issue date: 1971
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Mitotic regulators and their effects on Drosophila : chromosome structure during development
… other extreme, the sisters are held together (polytene). The endocycle itself can be modified and these variations are speculated to correlate with the observed differences in chromosome structure. In this thesis, we have analyzed the contribution of mitotic regulators to the endocycle and …
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Colocalization between Akirin, the NuRD Complex, and Histone Modifications
… deacetylase (NuRD) complex. <em>Drosophila</em> polytene chromosomes were used to visually identify colocalization between Akirin and subunits of the NuRD complex. The NuRD subunit, HDAC1, functions to remove acetyl groups from histone tails. Therefore, the colocalization between Akirin and …
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Evidence that the Boundary Element-Associated Factors BEAF-32A and BEAF-32B affect chromatin structure in Drosophila melanogaster
… of BID leads to a global disruption of polytene chromosome structure. Subsequent work using a fly line with a null mutation in the BEAF gene (BEAF AB-KO) also demonstrates a perturbation to polytene chromosome structure, although it is limited to the X-chromosome. Using Micrococcal …
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Mutational analysis of JIL-1, a tandem kinase in Drosophila
… a role in histone H3 serine 10 phosphorylation, polytene chromosome morphology maintenance, dosage compensation pathways, and homeotic transformation pathways. Due to the limitation of deletion/insertion mutations, EMS mutagenesis was performed to induce point mutations of JIL-1 and the mutation …
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Drosophila E2F and DP genes : their role in the regulation of G1-S progression and the activity of DNA replication origins
… pattern of S phase genes in mitotic and polytene cells of the embryo. A number of mutations in known and novel genes were isolated. Further analysis and phenotypic characterization has focused on the mutations that nearly eliminate the transcription of S phase genes in mitotic and …
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Comparative genomics of chromosomal rearrangements in malaria mosquitoes
… and whole genome amplification of polytene chromosomal fragments to obtain DNA for sequencing and assembly. The chromosomal regions spanning both breakpoints of the 2La in A. arabiensis and A. merus were laser microdissected from the polytene chromosomes. Subsequently, DNA samples …
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Chromosome evolution and mechanisms of speciation in the Anopheles gambiae complex
… spatial organizations of autosomal regions of polytene chromosomes in soma and germline cells. We found that molecular composition of pericentrometric autosome and sex chromosome repetitive DNA differs among sibling species of An. gambiae complex with highly similarity between An. coluzzii and …
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Genetic and cytological investigation of Nucleostemin-1 in Drosophila melanogaster
… several transcriptionally active inter-bands of polytene chromosomes. Over-expression caused melanotic tumors and larval and pupal lethality. RNAi depletion of NS1 caused a loss of imaginal (precursor) cells in the larval midgut, and an apparent block in the nucleolar release of large ribosomal …
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Liquid-on-liquid mixing for slide-based biological assays
… immunofluorescence staining of Drosophila larval polytene chromosomes by a factor of 100 in time and concentration, when compared to standard coverslip techniques. Flow visualization experiments reveal the fluid motions in the thin aqueous layer. Using time-lapse video photography to monitor the …
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Optimization of Cytogenetic and Physical mapping of Culicinae genomes
… Aedes and Culex genomes, the structure of their polytene chromosomes is damaged by ectopic contacts that make the analysis difficult. Mitotic chromosomes from imaginal discs of 4th instar larvae of Aedes aegypti were tested as a source for the physical genome mapping for this mosquito. …
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Analysis of the Role of Akirin Interactions with Nuclear Body Proteins During Myogenesis
… of both the CB and the HLB, were observed. Using polytene chromosome squashes and anti-Akirin immunostaining of a <em>coilin-eYFP</em> <em>Drosophila</em> line, co-localization of Akirin and Coilin was revealed, implying a physical interaction. These results shed light on the complex process of …
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Characterization of Boundary Element-Associated Factors BEAF-32A and BEAF-32B and Identification of Novel Interaction Partners in Drosophila Melanogaster
… (FRAP) to assess the dynamics of BEAF-32 on polytene chromosomes and also observed banding patterns, with the help of fluorescent protein labels, and evaluated its behavior during mitosis in early embryos. Finally, results obtained with BEAF prompted us to test for physical interactions …
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The genetic and molecular analysis of the mfs(2)31 locus in Drosophila melanogaster : a novel suppressor of position-effect variegation
… An in situhybridization to salivary gland polytene chromosomes revealed a P element in distal 31E of this strain. The P element, located in distal 31E, was cloned from thedysgenic mfs(2)31 allele. This element is responsible for the reduced transcription of a 1.2 kb message which is …
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Molecular visualization and localization of ribosomal subunits interaction in cells
… with nascent transcripts on Drosophila polytene chromosome and that amino acids are incorporated at transcription sites. My project aimed to investigate further the question of whether there are functional ribosomes in the nucleus; and also to confirm the presence of ribosomal proteins …
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Delineation of the molecular mechanisms underlying DNA replication initiation and changes in gene copy number during Drosophila development
… is a common mechanism of underreplication in polytene tissues. Our ORC localization studies also suggest that underreplication zones are hard-wired across tissues and that differential underreplication of these zones is dependent upon variation in fork progression across these regions. We then …
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Tracking translation factors in fission yeast nucleus
… of Drosophila melanogaster (D. melanogaster) polytene chromosomes. Whilst these findings might indicate the presence of ribosomal subunits at transcription sites, it has also been reported that these proteins associate with non-coding RNA in Saccharomyces cerevisiae (S. cerevisiae), suggesting …
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