Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"Polysome profiling"”.
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Host lncRNAs recognize invading bacteria and aid against infection by associating with polysomes
… been found to associate with ribosomes and polysomes; however, to date no research exists that addresses their role in translation regulation upon bacterial infection. AIM: The key question I wanted to answer during my PhD is whether host produced lncRNAs rewire the cell’s translation upon …
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Translational defects in multiple tissues from the Smn2B/- mouse model of SMA.
… and sequencing-based techniques (ribosome profiling and RNA-seq) coupled with biochemical and molecular biology-based assay (polysome profiling, co-sedimentation profiles, qPCR, and western blotting), which applied to study in molecular detail the translational defects in the brain, spinal …
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SURVIVING UNDER PRESSURE: TRANSLATIONAL REWIRING IN RESPONSE TO MECHANICAL STIMULI
… these tools with measures of translation rate, polysome profiling, ribogenesis, and protein degradation assays uncovered interesting evidences demonstrating a mechanism that couples the regulation of macromolecular production with rheological adaptation in colorectal cancer cells HCT116. In …
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Studies on the mammalian piRNA pathway in cancer cells
… tRNAs was also identified. Mass spectrometry and polysome profiling demonstrated that both Hiwi and Hiwi2 associate with components of the translational machinery, along with other partner proteins known to bind Piwis in the germline. This thesis demonstrates that the Piwi/piRNA pathway is active …
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The Role of Ribosomal Maturation Proteins WBSCR22 and EMG1 Methyltransferase Activity in Cancer
… of oncogenic protein translation. By using polysome profiling coupled with next generation sequencing I characterized genes that showed no differences in total mRNA expression levels but are less translated when the m7G modification is removed from the 18S rRNA. Among these genes, I …
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INTEGRATED TRANSCRIPTOMIC AND TRANSLATIONAL PROFILING OF EIF6-SBDS IN SHWACHMAN-DIAMOND SYNDROM REVEALS DIFFERENT CELLULAR STATES SHAPED BY RIBOSOME COMPOSITION AND FIDELITY
… matched SBDS-depleted models via shRNAi. Polysome profiling, RNA-seq, and Ribo-seq were used to assess how each perturbation influences translational output, decoding fidelity, and stress-response pathways. Additional analyses of rRNA fragments, snoRNA dynamics, and inferred …
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Characterizing novel molecular regulators of antiviral gene expression
… IFITM1 and MX1 that we had identified. Using polysome profiling and ribosome profiling, we determined that METTL3/14-regulated ISGs are translationally enhanced by METTL3/14. Additionally, ablation of putative m6A sites within the 3’UTR of IFITM1 decreased the translation of a reporter …
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Mining common genetic Variants impacting on Allele-Specific Translation and cancer risk
… of mTORC1 signaling and a p53 target. Polysomal profiling and Sanger sequencing revealed a higher abundance of the reference T allele in polysome-associated versus total RNA fractions of TA heterozygous cells, suggesting the two alleles exhibit a difference in translational efficiency. …
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Commonalities between SMA and ALS: investigation of ribosome heterogeneity and translational defects
… in SMA. SMN co-sediments with ribosomes and polysomes and acts as a RAP, influencing the translation efficiency of specific mRNAs. Computational analyses identified specific features in the sequences of transcripts bound by SMN-primed ribosomes: translational enhancer sequences in the 5’ …