Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Polysialic acid"”.
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Mechanism of the Neural Cell Adhesion Molecule and Mode of Polysialic Acid Adhesive Inhibition
… NCAM proteins to determine the effects of polysialic acid on both NCAM and C-cadherin mediated adhesion. Results indicate that polysialic acid inhibits both NCAM and cadherin adhesion by increasing the intermembrane repulsion. This inhibitory effect was dependant on both polysialic acid …
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Investigation of inhibitors of polysialyltransferase as novel therapeutics for neuroblastoma. Development of in vitro assays to assess the functionality and selectivity of novel small-molecule inhibitors of polysialyltransferases for use in neuroblastoma therapy
Polysialic acid is aunique carbohydrate that decorates the surface of the neural cell adhesion molecule. Polysialic acidis an onco-developmental antigen, expressed in tumours principally of neuroendocrine origin, notably neuroblastoma,strongly correlating with invasion and metastasis. …
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Pharmacological evaluation of the inhibition of polysialyltransferases as a therapeutic strategy in cancer. Characterisation of models for evaluating polysialic acid as a potential therapeutic target and pharmacological assessment of novel polysialyltransferase inhibitors
… for therapy of neuroblastoma relates to the polysialic acid decoration of the neural cell adhesion molecule (PSANCAM). PSA-NCAM is selectively re-expressed in a number of tumours including neuroblastoma, where it is thought to modulate tumour dissemination. Expression is strongly associated …
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DESIGNING A PLATFORM FOR DEVELOPING NOVEL GLYCAN-BINDING PROTEINS USING YEAST SURFACE DISPLAY
… of human fibronectin (FN3) was screened against polysialic acid, a glycan found in certain cancers, and enriched using a combination of random mutagenesis, magnetic-activated cell sorting, and fluorescence-activated cell sorting. The population of isolated binders was characterized by yeast …
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Function and Environmental Regulation ofkpsF, the First Gene of the Region One Operon of Escherichia Coli K1
… plaque size and decreased surface expression of polysialic acid. These results indicate that KpsF is not obligatory for capsule synthesis or transport, although KpsF may be necessary for normal transport and assembly of a wild type capsule. A potential nucleotide binding site and helix-turn-helix …
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Cloning, sequencing, and biochemical characterization of endo-N-acylneuraminidase
… hydrolyzes alpha-2,8-linked polysialic acid found on K1 encapsulated Escherichia coli and on the neural cell adhesion molecule (NCAM). A novel cloning strategy, which pursued ligation of partially restricted ALU 1 or SAU 3A genomic K1F DNA fragments, is described. Sequence …
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Synthesis of inhibitors of polysialyltransferases PST and STX. Development of routes to synthesis, preparation and purification of carbohydrate and carbacycle-based potential inhibitors of the polysialyltransferase enzymes PST and STX
PolySialic acid (polySia) is a linear carbohydrate homopolymer of α- 2,8-linked sialic acids and a posttranslational modification of NCAM (neural cell adhesion molecule), biosynthesized by combined action of two polysialyltransferase enzymes, ST8SiaIV(PST) and ST8SiaII(STX). PolySia alters …
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Sialylation as a mechanism for preferential Ligand binding in CFS1R
… Interestingly, gp130 also exhibits α2- 8 polysialic acid chains. Polysialylation has been previously associated with immunomodulation, but I have found that treating cells with an enzyme that can remove poly sialic acid potentiates proliferation of cells in a ligand dependent manner. This …
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Control of Inflammation Using Drug Delivery Strategies in in vitro Models
… factor decoys (TFD) are small nucleic acid sequences (~20 base pairs) that mimic the binding site for the TF on the native DNA, but do not encode for any proteins. By binding to the TF in the cytoplasm, TFD have potential to limit excessive immune signaling and inflammatory protein …
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Stress and Hippocampal Plasticity
… including dentate gyrus neurogenesis, polysialic acid neural cell adhesion molecule (PSA-NCAM) expression and neuronal excitability. In addition, the learning and memory process is dissected using a contextual fear conditioning paradigm in order to identify the component that can affect …
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Polysaccharide-Based Nanocarriers for Improved Drug Delivery
… as the basis for these nano-sized carriers. Polysialic acid (PSA) is such a polysaccharide with strong hydrophilicity that may reduce uptake by the reticuloendothelial system and prolong drug circulation. In this study, we developed PSA-based nanocarriers, specifically micelles and …
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New Carbohydrate-Based Anti-Cancer And Anti-Bacterial Vaccines
… for the quantification of various sialic acids expressed by cells and used to analyze the efficiency of N-phenylacetyl-D-mannosamine (ManNPhAc) to metabolically glycoengineer SKMEL-28 cancer cell. In specific, after cancer cells were cultured with ManNPhAc, the cells were treated with 2M …
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Design, Synthesis and Biological Evaluation of Inhibitors of Polysialyltransferases PST and STX. Design, synthesis and biological evaluation of a range of N-modified mannosamines, sialic acids and analogues from in silico screening as inhibitors of PolySia-NCAM biosynthesis with anti-migration activity.
… analogues, thio-linked CMP-sialic acid analogues, N-acyl modified sialic acids and compounds incorporating elements of both approaches. Novel methodology development in the synthesis of many of the compounds is described, notably a novel route to N-acyl sialosides. In addition, …