Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"Polypyrimidine Tract"”.
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Role of the Intron 13 Polypyrimidine Tract in Soluble Flt-1 Expression
… a series of point mutations were made in the polypyrimidine tract using pFIN13. After transient transfection, the levels of Flt-1 and sFlt-1 protein and mRNA were compared using quantitative PCR, RNA hybridization analysis, and protein immunoblotting. Results from quantitative PCR showed that …
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Investigation of the Polyprimidine Tract-Binding Protein-Associated Splicing Factor (PSF) Domains Required for the Hepatitis Delta Virus (HDV) Replication
… the host proteins that bind to its genome. The polypyrimidine tract-binding protein-associated splicing factor (PSF), an HDV interacting protein, was found to interact with the carboxy terminal domain (CTD) of RNA polymerase II (RNAPII), and to facilitate the interaction of RNA transcripts with …
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Novel Roles of PACS-1 Within the Nucleus and the Regulated Secretory Pathway
… and the nucleocytoplasmic RNA-binding protein polypyrimidine tract-binding protein 1 (PTBP1), unveiling a PACS-1 nuclear trafficking function. Second, we defined a role for PACS-1 orchestrating intracellular calcium levels within corticotropic cells. We establish PACS-1 interactions with …
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Glycine receptors in the developing rat spinal cord
… antigen-1 (Nova-1) and the brain isoform of the polypyrimidine tract binding protein (brPTB). Treatment of neurons in culture with antisense oligonucleotides to "knock down" one of the Nova-1 variants altered the expression of GlyR α2N. These results suggest that the relative levels of the …
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Interactions between Nova and brPTB Proteins in Alternative Splicing and Nuclear Localization
… is identified, that is closely related to the polypyrimidine tract-binding protein (PTB) and is ennched in the brain at the mRNA and protein levels. brPTB interacts with Nova proteins in vitro and in vivo. Splicing assays in kidney epithelial cell lines show that brPTB inhibits the effect of …
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Characterization of U2AF26, a Paralog of the Splicing Factor U2AF35
… U2AF35. U2AF65 recognizes the pyrimidine tract and U2AF35 binds to the AG dinucleotide, both of which are specific 3' splice site sequence motifs. In the present work U2AF26, a paralog of the conventional U2AF35, has been studied. U2AF26 shares 84% primary amino acid identity with U2AF35, …
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Mechanisms of Regulation of Tau Ires Mediated Translation
… tau. Out of these, I identified two proteins – polypyrimidine tract binding protein (PTB) and neural PTB (nPTB) as inhibitory ITAFs of tau IRES. Altering the expression of PTB and nPTB <em>in vitro</em> and in cells negatively influenced tau IRES activity and protein expression.</p> <p>Along …
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Unique Features of PTB RRMs: Insight into Protein Motions and RNA Binding
Polypyrimidine tract binding protein: PTB) is a highly conserved RNA binding protein comprised of four RRMs: RNA recognition motifs). RRMs are extremely prevalent in all kingdoms of life, and have been very well characterized in terms of structure and RNA binding properties. However, all four RRMs …
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Consequences of stochastic mrna synthesis in a gene regulatory pathway
… to be broken in situations where an intron's polypyrimidine tract is sequestered within a strong secondary structure. Furthermore, it was also found that, in the case of the alternative splicing of Sxl mRNA in female Drosophila cells, particular exon is removed from the transcript, due to the …