Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"Polyplex"”.
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Novel Lanthanide Containing Polymers for Nucleic Acid Delivery and Monitoring of Polyplex Dynamics
… Dynamic light scattering performed on the polyplexes (polymer-DNA complexes) indicate that they are in nanometer size range (50-80nm). All the polymers used to form polyplexes exhibited low toxicity to cultured human Glioblastoma cells (U-87) and showed variable transfection efficiency …
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Complexation Between DNA and Hydrophilic-Cationic Diblock Copolymers
DNA-polycation complexes (polyplexes) are gene delivery vehicles that offer a number of advantages over viral vehicles: high genetic payload capacity, low toxicity, and low cost. Among the many polycation architectures, hydrophilic-cationic diblock copolymers are particularly promising due to their …
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Cationic Glycopolymers for DNA Delivery: Cellular Internalization Mechanisms and Biological Characterization
… removal leads to decreased uptake. Individual polyplexes appear to have differing affinities for specific GAGs, as polyplex dissociation occurs in a charge-independent manner, and may influence binding. Internalization occurs through close interactions with GAGs, as GAGs accumulate on polyplex …
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Engineering of Nonviral Vectors for Gene Therapy
… small targeting and lysomotropic agents to the polyplex without chemical conjugation. By applying a hydrophobic analog of human insulin to the cyclodextrin polyplexes, I was able demonstrate a 2--3 fold enhancement in transfection efficiency in insulin receptor-rich cell lines.
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Bioreducible Polymer Nanosystems For Gene Delivery
… were synthesized. They complex with DNA forming polyplex nanoparticles and layer-by-layer (LbL) thin films as gene delivery vectors. Atomic force microscope (AFM), especially in situ real time AFM, provides a microscopic view of DNA release dynamics. It is shown that the depolymerization of …
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Design, development, and evaluation of novel polymer-drug conjugates and interpolymer complexes for the treatment of neurodegenerative disease.
… study aimed to develop novel PDCs and their nano-polyplexes as multifunctional therapeutic agents for AD treatment. Vanillin, an antioxidant, was conjugated to polyallylamine hydrochloride (NM10 and NM15), while the cholinesterase inhibitor naphthalimidohexylamine (HEXNAP) was conjugated to …
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Drug design strategies for modulating immune activity for the treatment of autoimmune diseases and cancer
… We previously demonstrated that this polyplex formulation can help increase tumor retention and limit systemic toxicity. Here, we investigated whether this polyplex formulation can be used in combination with anti-PD-1 therapy to instigate further anti-tumor effects. Together, each …
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Non-covalent Intermolecular Interactions in Polymer Design: Segmented Copolymers to Non-viral Gene Delivery Vectors
… measure the mass of GAGs that associated with a polyplex monolayer. Affinity of six different GAGs that varied in the charge density were measured for polyplexes formed with poly(galactaramidopentaethylenetetramine) (G4) cationic polymers and pDNA. Results showed that the affinity of GAGs for G4 …
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Development of polymer and lipid materials for enhanced delivery of nucleic acids and proteins
… uptake and trafficking mechanisms of PBAE/DNA polyplexes, and demonstrate that polyplex internalization and transfection depend on a key endo/lysosomal cholesterol transport protein, Niemann-Pick C1 (Npcl). Finally, working with cationic lipids termed lipidoids, which have shown exceptional …
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Mechanisms of Cytotoxicity and Intracellular Trafficking for Gene Delivery Polymers
… in the repeat unit of polymers play a role in polyplex formation. This research has also led us to a potential link between transfection efficiency and cytotoxicity; the polymers with the highest transfection efficiency were also the most toxic, and were also able to induce the most nuclear …
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Development of therapeutic agents for myotonic dystrophy type 1
… myotubes. In addition, the oligomer can form polyplexes with a locked nucleic acid (LNA) CAG16 gapmer, facilitating its cell uptake in both HeLa cells and DM1 patient myotubes, with a resulting decrease in the level of toxic r(CUG)exp significantly. RNA-seq analysis indicated that the …
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Characterization of Intermolecular Interactions in Nanostructured Materials
… to investigate the binding thermodynamics of polyplex formation with polycations and DNA. The imidazolium-containing and trehalose-based polycations were both found to have endothermic, entropically driven binding with DNA, while the adenine-containing polycation exhibited exothermic DNA …
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Tailoring Structure and Function of Imidazole-Containing Block Copolymers for Emerging Applications from Gene Delivery to Electromechanical Devices
… alkylation percentages, and relatively uniform polyplex sizes in aqueous media. The MTT assay developed cytotoxicity profiles with little toxicity prior to 83% alkylation. Finally, the luciferase expression assay revealed inefficient nucleic acid delivery to multiple cell types. Synthesis of …
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MRI and NMR Investigations of Transport in Soft Materials and Explorations of Electron-Nuclear Interactions for Liquid-State Dynamic Nuclear Polarization
… polymer chain content in a polymer/DNA complex (polyplex) formulation via one-dimensional proton NMR experiments. This assessment of free polymer quantity has critical impacts on in vivo gene transfection efficiency, cellular uptake, as well as toxicity of polycationic gene delivery vectors. …
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The effects of PVP(FE(III)) catalyst and polymer molecular weight on gene delivery via biodegradable crosslinked polyethylenimine
Human gene therapy has faced many setbacks due to the immunogenicity and oncogenity of viruses. Safe and efficient alternative gene delivery vehicles are needed to implement gene therapy in clinical practice. Polymeric vectors are an attractive option due to their availability, simple chemistry, …