Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"Polymerase enzyme"”.
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Structure and function of the human Poly(ADP-ribose) polymerase enzyme family
The poly(ADP-ribose) polymerase (PARP) family of enzymes in humans is comprised of 17 proteins. PARP-1, the first member of the family, synthesizes a large, complex post-translational modification, poly(ADP-ribose). While PARP-1 and some other PARPs have been extensively functionally characterized, …
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Molecular analysis of cyanobacterial RNA polymerase genes
The RNA polymerase genes rpoBC1C2 (the rpoB and rpoC2 are incomplete) of the cyanobacterium Nostoc commune have been cloned, sequenced, and expressed both in vivo and in vitro using E. coli systems. The rpo genes encode large subunits of DNA-dependent RNA polymerase. Two genes in N. commune, rpoC1 …
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Improved production of poly(hydroxybutyrate) and related enzymes
… the production of large quantities of the polymerase enzyme, PhaCRe. Therefore, a novel Escherichia coli cell factory, which can enter a non-growing but metabolically active quiescent state, was tested for high-efficiency PhaCRe production. Although the cells produced more PhaCRe, the …
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Novel strategies for DNA detection and assay.
… in the PCR reaction as this reaction uses a polymerase similar to that of Sanger’s sequencing reaction. There was no detectable incorporation of the compound. Studies of the toxicity of those derivatives, of the chelates and of the free lanthanide ions on the same polymerase enzyme were …
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The inhibition of M-MLV and HIV-1 reverse transcriptase by polyphenols extracted from the resurrection plant Myrothamnus flabellifolia (Welw.)
… of this thesis involved the development of a polymerase enzyme activity assay based on ethidium bromide fluorescence. A calibration curve for quantification of DNA was therefore prepared from the ethidium bromide fluorescence of Calf Thymus DNA. Results demonstrated that Calf Thymus DNA was a …
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Purification and Properties of Bacteriophage f2 Replicase
… of an RNA-dependent ribonucleotide-polymerizing enzyme (RNA replicase) produced during infection of E. coli with the RNA bacteriophage f2. Studies on the RNA replicase of the distantly-related bacteriophage Q3 have established the feasibility of using a simplified assay for replicase activity …