Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 277 for “"Polymerase chain reaction (PCR)"”.
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Method Verification of Cepheid Xpert Xpress Flu Assay by Polymerase Chain Reaction (PCR)
… of Cepheid Xpert Xpress Flu Assay by Polymerase Chain Reaction (PCR) was performed in accordance with California Pacific Medical Center (CPMC) laboratory protocol and Clinical and Laboratory Standards Institute (CLSI) Evaluation Protocol, EP15-A2. Method verification plan involves …
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Model microfluidic platform prototyping : design and fabrication of a Polymerase Chain Reaction (PCR) chip
Polymerase Chain Reaction (PCR) is a molecular biology method for the in vitro amplification of nucleic acid molecules, which has wide applications in the areas of genetics, medicine and biochemistry. MEMS technology offers several advantages for the miniaturization of biological protocols like …
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Technical evaluation of a Real-time polymerase chain reaction (PCR) assay for the detection of Bartonella spp for diagnostic purposes
… the utility of a previously published real-time PCR assay for detecting Bartonella spp. on cats. This will be the first time this target will be evaluated in a real-time PCR assay to detect Bartonella spp. in human samples. For this study, we constructed a plasmid vector containing an insert of …
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Assessment of Parasitism of House Fly and Stable Fly Pupae (Diptera: Muscidae) by Pteromalid (Hymenoptera: Pteromalidae) Parasitoids Using Polymerase Chain Reaction (Pcr)
… produced double bands prior to digestion of the PCR product by a restriction enzyme. The sizes of the bands corresponded to the sizes produced by amplification of the DNA of Muscidifurax and Spalangia species, possibly indicating hyperparasitism. Comparison of species composition in paired …
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Validation of a Pan-fungal polymerase chain reaction (PCR) assay for the detection and identification of medically important fungi in a diagnostic laboratory
… the performance of a selected pan-fungal PCR using 70 previously identified reference fungal isolates. The DNA yield and purity of three different DNA extraction methods was assessed, using 6 representative fungal isolates. The ZR Fungal/Bacterial DNA MicroPrep™ produced a median …
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The formulation and refinement of a polymerase chain reaction (PCR) assay for early diagnosis of paediatric HIV infection and genetic analysis of variants involved in vertical transmission of HIV-1
… presence of true infection is provided by the polymerase chain reaction (PCR) technique which allows the identification, and subsequent exponential amplification of low levels of specific viral nucleic acid using specific oligonucleotide primers. A major aim of this study was to develop and …
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Use of RAPD markers in plant varietal development
… varietal development. With the discovery of polymerase chain reaction (PCR), very low or single copy sequences can act as molecular markers because during the PCR, DNA is multiplied to such an extent that it can be easily visualized on an agarose gel. With the new technique of random …
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role of NF-<i>k</i>B signaling in multiple myeloma
… Cell Line JJN3, and BMF promoter region was PCR amplified and cloned into pCR 2.1 TOPO vector using One Shot® Chemically Competent E. coli. After confirmation of the clone through polymerase chain reaction (PCR) and restriction analysis, BMF promoter (2.5 Kb) was released from TOPO vector and …
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Elucidation of bipolar disorder : a convergent approach using genetics and imaging
… iii) genotype the candidate genes using a polymerase chain reaction (PCR) based technology, iv) analyse the genotyping data with the appropriate statistical methods, and v) obtain brain imaging data and perform the appropriate statistical analysis.
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Approaches to mitochondrial genome sequencing using the oxford nanopore minion device
Current DNA sequencing methods rely on polymerase chain reaction (PCR) to create sufficient copies of targeted DNA fragments to serve as a library. PCR and subsequent clean-up steps add considerable time and cost to the process and provide opportunity for introduction of amplification errors or …
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Molecular analysis of algal communities in the San Joaquin River
… algal communities in river water samples. Polymerase Chain Reaction (PCR) primers were designed to amplify the 18S rRNA gene of certain taxonomic groups of freshwater algae; there was limited success in specific amplification. Additionally, a primer pair utilizing both the 16S plastid gene …
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Return on investment and library complexity analysis for DNA sequencing
… sequencing artifacts and duplication events from polymerase chain reaction (PCR). The current study demonstrates improved return on investment (ROI) modeling strategies to better anticipate the impact of non-independent observations in multiple forms of next-generation sequencing data. Here, a …
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The low-level light therapy affects human dermal fibroblast via TGF-β/smad signaling pathway: in vitro study
… of LLLT 3 D. Cell viability assay 5 E. Real-time polymerase chain reaction (PCR) 5 F. Western blot analysis 5 G. Incubation with exogenous TGF-ß1 6 H. Statistical analysis 6 RESULTS 7 A. Cell viability assay 7 B. The effect of LLLT on the expression of mRNA of TGF-ß/smad signaling …
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Characterizing the distribution of planktonic fecal bacteria in the James River, Richmond, Virginia
… seasonally. Chapter 2 details the development of Polymerase Chain Reaction (PCR)-based methods to identify four common pathogenic taxa of fecal bacteria, and indicates that the factors controlling the distribution of these pathogens may be taxa-specific. Both studies, taken together, indicate that …
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Simultaneous partial Nitritation, Anammox and Denitrification (SNAD) process for treating ammonium-rich wastewaters
… by analysis of specific gene clone libraries, Polymerase Chain Reaction (PCR) and fluorescence in situ hybridization (FISH). SNAD process is a complex biological process where different populations with opposed environmental requirements coexist, thus, experiments have been performed with a lab …
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MULTIPLEX REAL TIME PCR and MELT CURVE ASSAY DEVELOPMENT FOR THE SIMULTANEOUS DETECTION AND IDENTIFICATION OF STREPTOCOCCUS PNEUMONIAE and STAPHYLOCOCCUS AUREUS
… this study, we developed a multiplex Real Time Polymerase Chain Reaction (PCR) assay to simultaneously detect <em>S. aureus</em> and <em>S. pneumoniae</em> in the same sample.</p>
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Identification of Differentially Expressed Genes in a Rat Unilateral Ureteral Obstruction Model
… annealing control primer(ACP)를 이용한 GeneFishingTM polymerase chain reaction(PCR)으로 관찰하였다. 그 결과, 발현 차이가 나는 총 37개의 유전자를 확인 할 수 있었으며, BLAST search를 통한 염기서열 분석 결과 총 37개 중 18개가 rat의 유전정보를 가지고 있었다. 그 중 retinol binding protein1(Rbp1), Pyridoxine 5'-phosphate oxidase, GADD45gamma, Tumor necrosis factor …
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Molecular genetics of the T cell receptor alpha chain
The polymerase chain reaction (PCR) was used in combination with oligonucleotide probe and nucleotide sequence analysis to examine T cell receptor (TCR) alpha chain diversity in fetal and adult thymi. Transcripts were amplified using various V$\alpha$ and C$\alpha$ specific primers and the cloned …
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Cytomegalovirus retinitis at Groote Schuur Hospital : clinical management and outcomes
… CMV retinitis undergo a vitreous biopsy for polymerase chain reaction (PCR) confirmation. Treatment consists of weekly intravitreal ganciclovir injections until clinical resolution has taken place. Patients not on highly active anti-retroviral treatment (HAART) are referred for initiation of …
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Detection of an Introduced Bacterial Culture in Greywater Treatment Reactors
… tools. Analysis of the 16S rRNA gene by polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) revealed community profiles of the dominant bacterial species. Species and strainspecific primers targeting either the 16S rRNA gene or the intergemc spacer (ITS) …
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