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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 755 for “"Polymerase Chain Reaction"”.
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A novel capillary polymerase chain reaction machine
I built a novel prototype capillary polymerase chain reaction machine. The purpose was to perform a single reaction as fast as possible with a reaction volume - 100 nl. The PCR mix is in the form of a 1 /1 droplet that moves between three heat zones inside of a 1 mm I.D. capillary filled with …
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A mathematical model of polymerase chain reaction induced stutter
… of DNA from different individuals, applying polymerase chain reaction (PCR) to the samples, and then running the amplified copies through capillary electrophoresis to produce a readout of peak heights corresponding to alleles on various loci. The alleles correspond to the number of repeats of …
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Polymerase chain reaction-based genotyping of swine MHC DQB and DRB alleles
… complex DQB and DRB loci using the PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) analysis technique is described. SLA DQB and DRB locus-specific oligonucleotide primers were designed to analyze expressed genes using the reverse transcription-polymerase chain …
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The cloning of novel gonadotropin-releasing hormone receptors by polymerase chain reaction
… mole and type II mouse and human GnRHRs using polymerase chain reaction (PCR) strategies. PCR was performed with degenerate primers designed to human type I GnRHR to areas that are not conserved between GPCRs in general, but are conserved between mammalian GnRHRs.
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Evaluation of a Paratuberculosis Quantitative Polymerase Chain Reaction Assay with Microscopic Correlation
… with a commercial paratuberculosis quantitative polymerase chain reaction (qPCR) assay and had a sensitivity of 92%. To determine assay specificity, 21 FFPE tissues from animals without gastrointestinal disease combined with 13 FFPE tissues from non-ruminant animals (frog, dove, turtle, dog, and …
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DNA Fingerprinting: Identification of Organisms Using the Polymerase Chain Reaction and Various Primers
… 5 ?l of fruit DNA extract in the PCR reaction yielded variable results whereby in some cases, such as with the analu primers, either fewer bands were seen compared to 1 and 10 ?l of DNA, or no bands were visible at all, thus providing less meaningful data. Like RFLP and RAPD analysis, …
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Method Verification of Cepheid Xpert Xpress Flu Assay by Polymerase Chain Reaction (PCR)
… of Cepheid Xpert Xpress Flu Assay by Polymerase Chain Reaction (PCR) was performed in accordance with California Pacific Medical Center (CPMC) laboratory protocol and Clinical and Laboratory Standards Institute (CLSI) Evaluation Protocol, EP15-A2. Method verification plan involves …
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Detection of Feline Leukemia Virus in Feline Bone Marrow Using Polymerase Chain Reaction
… The purpose of this study was to determine if polymerase chain reaction (PCR) could detect FeLV proviral DNA in bone marrow and provide a method of detecting latent infections. Blood and bone marrow samples from fifty cats and bone marrow from one fetus were collected; sixteen had …
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Detection and Identification of Acinobacillus pleuropneumoniae serotype 5 by multiplex Polymerase Chain Reaction
… To avoid the complications of antibody-antigen reactions, a PCR assay was developed to detect Actinobacillus pleuropneumoniae and identify serotype 5 strains. Primers specific to the conserved capsular export region of A. pleuropneumoniae amplified a 0.7 kb DNA band in all strains with the …
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Determination of soybean cultivar resistance to soybean cyst nematode with quantitative polymerase chain reaction
… roots by H. glycines with real-time quantitative Polymerase Chain Reaction (qPCR), a prelude to differentiation of resistance levels in soybean cultivars. Two experiments were conducted. In the first one, a consistent inoculation method was developed using to provide active second-stage juveniles …
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Model microfluidic platform prototyping : design and fabrication of a Polymerase Chain Reaction (PCR) chip
Polymerase Chain Reaction (PCR) is a molecular biology method for the in vitro amplification of nucleic acid molecules, which has wide applications in the areas of genetics, medicine and biochemistry. MEMS technology offers several advantages for the miniaturization of biological protocols like …
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Bovine embryo microinjection, culture, microsurgery, and DNA analysis by the polymerase chain reaction technique
… from bovine morulae for DNA amplification by the polymerase chain reaction technique (PCR), and evaluated the use of DNA microinjection, in vitro culture, morula bisection, and PCR for production and selection of transgenic bovine preimplantation embryos. In vivo fertilized one-cell bovine embryos …
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A Rapid and Power-Efficient Laser-Induced Graphene Micro-Thermocycler for DNA Polymerase Chain Reaction
… billions of copies of a DNA’s specific segment, polymerase chain reaction (PCR) has a wide range of applications in DNA cloning, forensics, medical, and infectious disease detection. As a result, the technology is becoming more and more widespread as the COVID-19 pandemic pushes for the need for …
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Application of Multiplex Reverse-Transcriptase Polymerase Chain Reaction for Identification of Leukemia Associated Gene Abnormalities
PUOPOSE: The best prognostic predictor for acute leukemia is known to be the finding of genetic abnormalities of leukemic cells. Methods for detecting the genetic abnormalities include chromosomal studies for karyotyping, FISH(Fluorescence in situ hybridization) and RT-PCR. However, each methods …
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Feline Leukemia Virus Detection in Corneal Tissues of Cats by Polymerase Chain Reaction and Immunohistochemistry
… of FeLV proviral DNA and gp70 antigen by polymerase chain reaction (PCR) and immunohistochemical (IHC) testing, respectively. Eleven (64.7%) positive corneal PCR results were obtained from 17 ELISA positive cats. Of 12 cats which were both ELISA and IFA positive on peripheral blood, 10 …
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The development and evaluation of a polymerase chain reaction assay for the diagnosis of mycobacterium tuberculosis infections
… similar to that of Ziehl-Neelsen stain. The polymerase chain reaction (PCR) has been shown to exhibit extreme sensitivity and specificity for a wide variety of infectious agents including virus, bacteria and protozoans. There have also been a few promising reports of PCR assays for …
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Detection of Actinobacillus Pleuropneumoniae and Identification of Serotypes 1, 2, and 8 by Multiplex Polymerase Chain Reaction
… 6 and 8. To avoid these serological cross-reactions, a multiplex PCR assay was developed to detect A. pleuropneumoniae and identify serotypes 1, 2, and 8. Primers specific to the conserved capsular polysaccharide export region of A. pleuropneumoniae serotype 5 amplified a 880 bp fragment in …
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