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Showing 1 to 17 of 17 for “"Polyhydroxybutyrate (PHB)"”.
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Studies on the mechanism of polyhydroxybutyrate (PHB) granule formation in Ralstonia eutropha H16/
Polyhydroxybutyrates (PHBs) are biodegradable polymers produced by bacteria in the form of insoluble granules for carbon storage. Insights into the mechanisms of granule formation are important for improving commercial PHB production and engineering new alternatives to petroleum-based plastics. …
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The role of the phasin PhaP in promoting polyhydroxybutyrate (PHB) granule formation in Caulobacter crescentus
Polyhydroxybutyrate (PHB) is a biodegradable polymer produced by many microorganisms when they find themselves limited in an essential nutrient such as nitrogen or phosphorus but in the presence of an abundant carbon source. PHB and other related polymers are of interest because they have valuable …
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Mechanistic investigation of polyhydroxybutyrate (PHB) synthases and elucidation of PHB biosynthesis and degradation process in Wautersia eutropha H16
Polyhydroxyalkanoate (PHA) synthase from various bacterial organisms is able to catalyze the polymerization of (R)-hydroxyalkanoate-CoAs into high molecular weight PHAs under nutrient- limited conditions in the presence of a carbon source. PHA synthases are representative of enzymes involved in …
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Isolation and characterization of bacterial polyhydroxybutyrate inclusions
Polyhydroxybutyrate (PHB) is a carbon reserve found in some bacteria, and under nutrient limiting conditions accumulates intracellularly in the form of inclusion bodies. These inclusions contain proteins, and the PHB within the inclusions exists in an amorphous state. In this study a procedure to …
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Molecular weight modulation in polyhydroxybutyrate fermentations
Polyhydroxybutyrate (PHB) is a material with significant potential for commercial applications. It has material properties similar to isotactic polypropylene; it can be produced from renewable resources; it is biodegradable. Unfortunately, it is very brittle when compared to polypropylene. The …
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Control of product molecular weight in the enzymatic synthesis of a biodegradable polyester, polyhydroxybutyrate
Polyhydroxybutyrate (PHB) is a polymer accumulated by many bacterial species to store carbon and reducing equivalents. PHB is packaged intracellularly in inclusions termed "granules" that contain the polymer in an amorphous state, surrounded by a protein coat. The molecular weight (Mw) of PHB is …
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The bacterial synthesis and characterisation of polyhydroxyalkanoates using waste plastics as carbon sources for novel applications
… Cupriavidus necator H16 was used to generate polyhydroxybutyrate (PHB) and other PHAs with differing polyester compositions, depending on the type of waste carbon source used in 48 hour fermentations in nutrient rich media. These products were then chemically analysed to assess their …
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Production of biopolymer using a membrane-based bioreactor
… methanotrophic bacteria produce intracellular polyhydroxybutyrate (PHB) in the absence of nitrogen and presence of methane and oxygen. In this study, Colorado School of Mines (CSM) and Mango Materials (MM) collaborated on a Phase I NASA grant, investigating the production of PHB in a novel …
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Evolution of energy storage traits in symbiotic rhizobia
… nitrogen-fixation into storage compounds like polyhydroxybutyrate (PHB). Although the conflict-of-interest between PHB accumulation and nitrogen-fixation has been discussed as a driving factor in the evolution of legume-rhizobia interactions, its role in natural populations is unclear. …
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The Utilization of Activated Sludge Polyhydroxyalkanoates for the Production of Biodegradable Plastics
… amounts of the PHA copolymers formed, i.e., polyhydroxybutyrate (PHB) and polyhydroxyvalerate (PHV) were different under different operating conditions, even though the types and amounts of volatile fatty acids (VFAs) in the feed were the same. It was determined that high total phosphorus …
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Systematic investigation of potential factors that affect the production costs of the bio-based and bio-degradable plastic polyhydroxyalkanoates (PHAs) by a costing analysis based on early process simulation
… was simulated is the most characterised PHA, polyhydroxybutyrate (PHB).
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Screening and Engineering Phenotypes using Big Data Systems Biology
… of yeast was quantified by the production of polyhydroxybutyrate (PHB) using three heterologous enzymes. The resulting assay demonstrated the differences in acetyl-CoA availability in the peroxisomes under various culture conditions and genetic alterations. 2) To assist in the design of …
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Implications of Microorganisms in Future Crewed Missions to Mars
… mg/L/h for a Bacillus subtilis culture yielding polyhydroxybutyrate (PHB) biopolymer. A photobioreactor capable of growing large volumes of C. reinhardtii biomass was developed, and different techniques evaluated to estimate the starch content during culture. An optimised protocol for collecting …
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Conversion of waste organic carbon and nutrient streams to renewable fuels through integrated biological funneling and catalytic hydrothermal upgrading
… process utilizing the biological polymer polyhydroxybutyrate (PHB; a storage product of mixed cultures selected from activated sludge) as feedstock to produce gasoline-grade liquid hydrocarbons. The goal of this thesis is to advance the overall understanding of technological pathways for …
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Valorization of wastewater-derived biomass via integrated aqueous systems
… of such systems; and (b) wastewater-derived polyhydroxybutyrate-accumulating bacteria (WW-PHB) systems, where pilot-scale production of the biopolymer PHB has been demonstrated with no impact to effluent discharge quality, yet critical barriers remain in identifying competent technologies for …
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Produkce polyhydroxyalkanoátů z vybraných odpadních substrátů
Predložená bakalárska práca sa zameriava na štúdium produkcie polyhydroxyalkanoátov (PHA) baktériami Burkholderia sacchari a Burkholderia cepacia a taktiež faktorov, ktoré produkciu ovplyvňujú. Hlavnou skúmanou oblasťou bola produkcia PHA na substráte z kukuričnej siláže. V teoretickej časti bola …
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Exploring genomic complementarity in a naturally occurring coculture: A case study of Methylocystis echinoides rim and Hyphomicrobium sulfonivorans
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2025-10-19 without embargo terms