Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 17 of 17 for “"Polyethylenimine (PEI)"”.
-
Syntheses, structures and properties of novel poly(amino ester)s
… efficiency for DNA delivery comparable to polyethylenimine (PEI). Furthermore, some hyperbranched poly(amino ester)s can emit blue fluorescence. These poly(amino ester)s should be applicable for safe and efficient gene and drug delivery and as novel biocompatible fluorophores.
-
Elucidation of Design Criteria for Non-Viral Gene Delivery Vector Development
… DNA and the polymeric gene delivery agent polyethylenimine (PEI). The results indicate that weakened polymer/DNA interactions increase gene delivery efficiency and, more generally, that the strength of polymer/DNA interactions needs to be considered and optimized when designing non-viral …
-
Investigation of barriers to non-viral gene delivery and design of novel polymer-based gene delivery systems
… we investigated the gene transfer properties of polyethylenimine (PEI), a highly effective non-viral vector material. We demonstrated that the transfection efficiency of this polymer is due to its ability to avoid lysosomal degradation as a result of its buffering capacity, providing quantitative …
-
Investigation of microbicidal activity of surface-immobilized hydrophobic polycations
… The resultant, covalently-immobilized, Nalkyl polyethylenimine (PEI) demonstrates complete bactericidal activity against S. aureus and E. coli (i.e., representative Gram-positive and Gram-negative bacteria, respectively). In addition, by utilizing light to activate the covalent cross-linking, …
-
Understanding Non-viral Nucleic Acid Delivery Vehicles with Different Charge Centers and Degradation Profiles
… the buffering capacity to endosomal release of polyethylenimine (PEI) based polyplexes, which could contribute to high transfection efficiency. Secondary amine structures rendered PGAAs buffering capacity around physiological pH. To test the feasibility of the mechanism for PGAAs, new no …
-
FLUORESCENT PROBE INVESTIGATIONS OF MICROENVIRONMENTS OF ANALYTICAL INTEREST (REVERSED-PHASE, POLYETHYLENIMINE, POLARIZATION)
… using a three component system: metals bound to polyethylenimine (PEI) and pyrenesulfonate counterion probes. Metals altered rates of excited state processes and defined binding environment. Variables were net charge on metal and probe and metal-amine complex properties. Cu(II)-PEI efficiently …
-
Plastic Waste-Derived CO2 Sorbents and Methods for Enhanced Selectivity and Stability
… sorbents, an amine-containing polymer, polyethylenimine (PEI), was added to the carbon sorbent in Chapter 4. To do this, we first modulated the pore structure of the sorbent to become more mesoporous through a liquid-salt templating mechanism. More mesoporosity allowed better …
-
Development of plasmid dna formulations for application as microspheric dna vaccines
… preservation of DNA stability. The use of polyethylenimine (PEI) and stearylamine (SA) as agents in the microspheric formulation of plasmid DNA is a novel approach to DNA vaccine design. Using these molecules as model positively-charged agents, their influence on the characteristics of the …
-
Development of Forward Osmosis Based Separations: A Novel Approach in Membrane Technology
… mechanism have been tuned by introducing Polyethylenimine (PEI) on the membrane. Zwitterionic monomer, AEPPS was used to introduce the polyamide layer by conventional in-situ modification and second interfacial polymerization (SIP), respectively. The excellent antifouling surface was …
-
Upconversion Nanoparticles for pH Sensing and Imaging
… particles were modified with different ligands, polyethylenimine (PEI) and poly(isobutylene-alt-maleic anhydride) (PMA), respectively, to enable dispersion in aqueous solutions. The effect of two anthraquinone dyes, Calcium Red and Alizarin Red S, on UCNPs was measured by spectroscopic techniques …
-
Specific and efficient in vivo delivery of DNA and siRNA by polyethylenimine and its derivatives
Linear polyethylenimine (PEI) is the "gold standard" of polycationic gene delivery vectors. However, little focus has been placed on enhancing or understanding the specificity of PEImediated gene delivery. Herein we evaluated the effect of chemical modification on the specificity of PEI-mediated …
-
Nanofibrillation of lignocellulosic biomass into lignin-containing nanocellulose for 3D printing and antimicrobial applications
… groups that introduced reactivity with polyethylenimine (PEI). The as-synthesized PEI-LNC showed attractive antioxidant and antibacterial properties, and the lignin residues played negatively against those functions but provided a beneficial anti-aggregation property. With enzymatic …
-
Prominent Platforms for the Synthesis of Size-Controllable Polymeric Nanoparticles for Gene Delivery
… PNPs consist of perfluoroheptanoic acid (PFHA), polyethylenimine (PEI), and low molecular weight heparin (LHP). These components can condense nucleic acids into compact nanopar- ticles (NPs) while maintaining robust stability and minimal cytotoxicity. To minimize thebatch-to-batch heterogeneity …
-
Nanoclay-Based Solid-Amine Adsorbents for Carbon Dioxide Capture
… groups of the clay. While another amine, polyethylenimine (PEI), was attached to the surface of the clay by electrostatic interactions. To confirm the attachment of amines to the clay, the samples were characterized using FTIR and the corresponding peaks for amines were observed. The …
-
Quo vadis: quantitative evaluation of intracellular kinetics of siRNA delivered by poly-and-lipoplexes
… of some of the most common delivery materials: polyethylenimine (PEI) and N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate (DOTAP) liposomes with various amounts of cholesterol as “helper lipids.” In order to gain new insights into the endocytosis of siRNA-based synthetic …
-
Design of chemically treated activated carbon fibers for mercury removal and advanced membranes for water purification
… copolyimide asymmetric membranes using branched polyethylenimine (PEI) at different reaction temperatures was studied. The rejection sequence of CaCl2 > NaCl > Na2SO4 indicated a positively charged membrane surface. Additionally, the resultant membranes were very stable in dimethyl formamide …
-
Effect of uptake pathway on non-viral gene delivery in vitro and in vivo
… endocytosis is important to in vitro polyethylenimine (PEI)- and polyamidoamine (PAMAM)-mediated gene delivery in HeLa cells through the use of small molecule drugs and small-interfering RNA [1-2]. The goal of this thesis is to further elucidate the effects of cellular uptake mechanism …