Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"Polycomb repressive complex 2 (PRC2)"”.
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Gsk3Beta-Mediated Ezh2 Phosphorylation Suppresses Methylation of H3K27 and Ezh2’S Oncogenic Functions
… and the enzymatic core subunit of polycomb repressive complex 2 (PRC2), promotes cell growth and migration through catalyzing trimethylation of histone H3 at Lys 27 (H3K27me3) and plays an important role in tumorigenesis. Its expression can be controlled by phosphorylation. However, …
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Using "Designer" Nucleosomes to Study Enzymatic Crosstalk Between Histone Ubiquitylation and Histone Methyltransferases
… in depth. I have been interested in how the complex repertoire of histone PTMs are co-regulated to generate combinations with meaningful physiological outcomes. One important mechanism is "crosstalk" between pre-existing histone PTMs and enzymes that add or remove subsequent modifications on …
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THE IMPACT OF REPLICATION STRESS ON THE EPIGENOME OF MOUSE EMBRYONIC STEM CELLS
… remain unclear. Preliminary data indicated that polycomb repressive complex 2 (PRC2)-targeted genes are aberrantly activated upon replication stress in mESCs. Genome-wide chromatin immunoprecipitation of suppressive and active histone marks revealed a global loss of H3K27me3 and the maintenance …
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Ezh2 T416 Phosphorylation Enhances Breast Cancer Tumorigenesis
… homologue 2 (EZH2) is the catalytic subunit of Polycomb repressive complex 2 (PRC2) and catalyzes the trimethylation of histone H3 on lysine 27 (H3K27Me3), to repress gene transcription. Many types of cancer stem and progenitor cells, including breast, have demonstrated EZH2 to be fundamental in …
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EZH1 variants disrupt neurogenesis in hPSC-derived neuronal models and cause neurodevelopmental disorders
… 27 (H3K27) methyltransferases, and part of the Polycomb Repressive Complex 2 (PRC2). Its paralogue, EZH2, is essential in maintaining transcriptional repression of non-lineage specific genes during development. In neurodevelopment, EZH2 is highly expressed in dividing cells, and its dysfunction …
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The Role of Polycomb Repressive Complex 2 in Epidermal Homeostasis and Hair Growth
Polycomb repressive complex 2 (PRC2) catalyses the methylation of ‘Lys-27’ of histone H3, leading to transcriptional repression of target genes through its catalytic subunit Enhancer of zeste homolog 1/2 (EZH1/2). PRC2 functions as a critical regulator of stem cells in mouse embryonic and adult …
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CRAMP1 drives linker histone expression to enable Polycomb repression
… for linker histones in the function of Polycomb Repressive complex 2 (PRC2) in human cancer cell lines. Through a CRISPR/Cas9 genetic screen in a fluorescent reporter cell line responsive to PRC2 perturbation, I identified an essential requirement for the poorly characterised gene CRAMP1 …
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Genetic and Epigenetic Characterisation of Breast Tumours
… addition, we found high gene expression of the Polycomb repressive complex 2 (PRC2)-member EZH2 and low methylation frequency in basal-like tumours, indicating alternative epigenetic silencing mechanism in these tumours. EZH2 is the key member of PRC2 that catalyses the histone modification …
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The role of Polycomb-mediated epigenetic regulation in embryonic stem cell differentiation
… gene expression networks for this process? Polycomb group (PcG) proteins are epigenetic regulators necessary for proper differentiation of cells and for mammalian development. Notably, faulty regulation of PRC2 has been associated with a broad range of cancers, suggesting that it has a …
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Epigenetic Regulation Controls Intrinsic and Extrinsic Drivers of Gastrointestinal Development
… control epithelial fate and function through Polycomb Repressive Complex 2 (PRC2)-mediated chromatin bivalency. While key lineage-determining genes possess tissue-specific patterns of chromatin accessibility, PRC2 controls WNT ligand expression in mesenchymal cells without altering …
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EPIGENETIC MECHANISMS OF POLYCOMB REPRESSIVE COMPLEX 1 IN INTESTINAL LINEAGE COMMITMENT
Polycomb group proteins are key transcriptional repressive complexes involved in cell fate decisions during development and cell identity maintenance during homeostasis (1). Polycomb group proteins primarily assemble into two different complexes: Polycomb repressive complex 2 (PRC2) with the …
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Role of Polycomb Group Protein Mel18 in Hematopoietic Stem Cell Maintenance
Polycomb group (PcG) proteins are epigenetic gene silencers that have been implicated in stem cell maintenance and cancer development. Genetic and biochemical studies indicate that Polycomb group proteins exist in at least two protein complexes, Polycomb repressive complex 2 (PRC2) and Polycomb …
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Functional analysis of candidate flowering time genes from the model legume Medicago truncatula
… (winter cold) conditions. A putative Medicago Polycomb Repressive Complex 2 (PRC2) subunit VERNALISATION2 (MtVRN2) was characterised previously in the Putterill laboratory, whose mutation led to early flowering. This indicated that MtVRN2 represses the transition to flowering in Medicago, …
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Novel pathways of human host defence against Mycobacterium tuberculosis
… for TB. Finally I elucidated the role that Polycomb Repressive Complex 2 (PRC2) plays in the H3K27me3 epigenetic suppression of nitric oxide (NO•) production downstream of Nitric Oxide Synthase 2 (NOS2) transcription in human macrophages. I determined that the inhibition of NO• production …
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PRC2 GATEKEEPS THE BALANCE BETWEEN DIRECT AND INDIRECT NEUROGENESIS AND CONTROLS NEURONAL MIGRATION DURING HUMAN CORTICOGENESIS
… was identified in heterozygous mutations in Polycomb repressive complex 2 (PRC2) components. This complex catalyzes the trimethylation of Lysine 27 on histone 3 (H3K27me3), thereby repressing transcription during embryonic development. However, our understanding of how PRC2 dysfunction …
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Paraspeckle protein NONO regulates active chromatin by allosterically stimulating NSD1
… distinguishing transcriptionally active from repressive chromatin domains. These modifications are catalyzed by distinct families of histone methyltransferases: the Nuclear Receptor–Binding SET Domain Protein (NSD) family for H3K36me2 and the Polycomb Repressive Complex 2 (PRC2) for H3K27me3. …
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The genome-wide analysis of the specification and activation of human chromosomal DNA replication origin sites
… Remodelling and histone Deacetylation (xNuRD) complex are essential for DNA replication initiation, but the mechanism(s) involved remain unelucidated. The precise mechanisms of DNA replication, and factors involved in human replication origin specification and activation are not fully known. My …
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Single-Molecule Investigation of Chromatin-Associated Factors in Genome Organization and Epigenetic Maintenance
… layer of eukaryotic gene regulation lies in the complex structure of chromatin. This scaffold of structural proteins and enzymatic regulators determines what genes are expressed at what times, leading to cell differentiation, cell fate, and often disease. Currently, the field of chromatin biology …
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CATALYTIC-INDEPENDENT FUNCTIONS OF KDM6A IN B CELL MATURATION AND IMMUNE REGULATION
… H3 lysine-27, counteracting the function of Polycomb Repressive Complex 2 (PRC2). Kdm6a plays a key role in transcriptional activation of genes that control cell differentiation during embryonic development and postnatal life. It is recurrently mutated in several solid and hematological …
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Fasting alters histone methylation in paraventricular nucleus of chick through regulating of polycomb repressive complex 2
… respectively. EZh2 (enhancer of zeste 2) in the polycomb repressive complex 2 (PRC2) is the only methyltransferase that has been linked to catalyze this methylation reaction. In addition, SUZ (suppressor of zeste) and EED (embryonic ectoderm development) are two other key proteins in PRC2 …
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