Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"Polycomb Repressive Complex"”.
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EPIGENETIC MECHANISMS OF POLYCOMB REPRESSIVE COMPLEX 1 IN INTESTINAL LINEAGE COMMITMENT
Polycomb group proteins are key transcriptional repressive complexes involved in cell fate decisions during development and cell identity maintenance during homeostasis (1). Polycomb group proteins primarily assemble into two different complexes: Polycomb repressive complex 2 (PRC2) with the …
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Roles of Polycomb Repressive Complex 2 in differentiation and lineage commitment
Contains fulltext : 310092.pdf (Publisher’s version ) (Open Access)
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Discovery and Characterization of the Polycomb Repressive Complex 1 of C. Elegans
… of MIG-32 a RING protein similar to some Polycomb proteins that were identified in a yeast two hybrid screen with UNC-3 as bait. The Polycomb repression complex 2 (PRC2) methylates histone H3 lysine 27 at target genes to modify gene expression, and this mark is recognized by PRC1, which …
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The Role of Polycomb Repressive Complex 2 in Epidermal Homeostasis and Hair Growth
Polycomb repressive complex 2 (PRC2) catalyses the methylation of ‘Lys-27’ of histone H3, leading to transcriptional repression of target genes through its catalytic subunit Enhancer of zeste homolog 1/2 (EZH1/2). PRC2 functions as a critical regulator of stem cells in mouse embryonic and adult …
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DEFINING THE ROLE OF THE PCGF3-CONTAINING POLYCOMB REPRESSIVE COMPLEX 1 IN SYNOVIAL SARCOMA
… the primary oncogenic driver, disrupting BAF complexes and redirecting them to Polycomb repressive domains, thus activating them. This mislocalization depends on the higher affinity of the fusion oncoprotein for H2AK119ub, the histone modification deposited by Polycomb Repressive Complex 1 …
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Investigating the role of the Polycomb Repressive Complex 2 in human in vitro pancreatic differentiation
… the in vitro cells. I show that manipulation of Polycomb protein-mediated H3K27me3 levels in differentiation at specific time-points has a variable effect on end-cell population, with an apparently negative effect early in differentiation, but with potentially a more positive effect later in the …
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Fasting alters histone methylation in paraventricular nucleus of chick through regulating of polycomb repressive complex 2
… respectively. EZh2 (enhancer of zeste 2) in the polycomb repressive complex 2 (PRC2) is the only methyltransferase that has been linked to catalyze this methylation reaction. In addition, SUZ (suppressor of zeste) and EED (embryonic ectoderm development) are two other key proteins in PRC2 …
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Multiomic Analyses Reveal Epigenomic Alterations and New Targets of Polycomb Repressive Complex 2 in Schwann Lineage Cells and Malignant Peripheral Nerve Sheath Tumors
… of histone H3 on lysine 27 (H3K27me3), a repressive epigenetic mark. Consequently, PRC2 dysfunction may induce significant epigenomic and gene expression alterations. Our research aimed to understand mechanisms underlying benign-to-malignant tumor transformation for improved diagnosis and …
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The Role of Ring1 and Rnf2 in Skeletal Muscle Regeneration
The skeletal muscle environment is highly complex, with inputs from multiple cell types and environmental challenges in homeostatic and regenerative conditions. Satellite cells, a population of quiescent adult stem cells, maintain the regenerative potential of muscle well into adulthood, but the …
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Role of Polycomb Group Protein Mel18 in Hematopoietic Stem Cell Maintenance
Polycomb group (PcG) proteins are epigenetic gene silencers that have been implicated in stem cell maintenance and cancer development. Genetic and biochemical studies indicate that Polycomb group proteins exist in at least two protein complexes, Polycomb repressive complex 2 (PRC2) and Polycomb …
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Novel pathways of human host defence against Mycobacterium tuberculosis
… for TB. Finally I elucidated the role that Polycomb Repressive Complex 2 (PRC2) plays in the H3K27me3 epigenetic suppression of nitric oxide (NO•) production downstream of Nitric Oxide Synthase 2 (NOS2) transcription in human macrophages. I determined that the inhibition of NO• production …
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Mapping the core regulatory circuitry of embryonic stem cells
… We then determined the genes targeted by the Polycomb Repressive Complex in ES cells. These datasets allowed us to define the core transcriptional regulatory circuitry for these cells and demonstrated that pluripotency is mediated through the repression of developmental regulators. Finally, an …
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Gsk3Beta-Mediated Ezh2 Phosphorylation Suppresses Methylation of H3K27 and Ezh2’S Oncogenic Functions
… and the enzymatic core subunit of polycomb repressive complex 2 (PRC2), promotes cell growth and migration through catalyzing trimethylation of histone H3 at Lys 27 (H3K27me3) and plays an important role in tumorigenesis. Its expression can be controlled by phosphorylation. However, …
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Using "Designer" Nucleosomes to Study Enzymatic Crosstalk Between Histone Ubiquitylation and Histone Methyltransferases
… in depth. I have been interested in how the complex repertoire of histone PTMs are co-regulated to generate combinations with meaningful physiological outcomes. One important mechanism is "crosstalk" between pre-existing histone PTMs and enzymes that add or remove subsequent modifications on …
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THE IMPACT OF REPLICATION STRESS ON THE EPIGENOME OF MOUSE EMBRYONIC STEM CELLS
… remain unclear. Preliminary data indicated that polycomb repressive complex 2 (PRC2)-targeted genes are aberrantly activated upon replication stress in mESCs. Genome-wide chromatin immunoprecipitation of suppressive and active histone marks revealed a global loss of H3K27me3 and the maintenance …
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E2F6 in axial skeletal development and gliosis
… a repressor of transcription in concert with the polycomb group (PcG) proteins and chromatin modifiers. PcG proteins regulate processes required for proper embryonic development and differentiation. E2F6 interacts with core components of Polycomb Repressive Complex 1 (PRC1) and participates in …
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Ezh2 T416 Phosphorylation Enhances Breast Cancer Tumorigenesis
… homologue 2 (EZH2) is the catalytic subunit of Polycomb repressive complex 2 (PRC2) and catalyzes the trimethylation of histone H3 on lysine 27 (H3K27Me3), to repress gene transcription. Many types of cancer stem and progenitor cells, including breast, have demonstrated EZH2 to be fundamental in …
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EZH1 variants disrupt neurogenesis in hPSC-derived neuronal models and cause neurodevelopmental disorders
… 27 (H3K27) methyltransferases, and part of the Polycomb Repressive Complex 2 (PRC2). Its paralogue, EZH2, is essential in maintaining transcriptional repression of non-lineage specific genes during development. In neurodevelopment, EZH2 is highly expressed in dividing cells, and its dysfunction …
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CRAMP1 drives linker histone expression to enable Polycomb repression
… for linker histones in the function of Polycomb Repressive complex 2 (PRC2) in human cancer cell lines. Through a CRISPR/Cas9 genetic screen in a fluorescent reporter cell line responsive to PRC2 perturbation, I identified an essential requirement for the poorly characterised gene CRAMP1 …
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TOWARD STRUCTURAL INSIGHTS INTO THE RECOGNITION OF H3K27 TRIMETHYLATION BY CANONICAL PRC1
Polycomb group (PcG) proteins are chromatin modifiers that epigenetically regulate the transcription of key developmental genes involved in embryogenesis, pluripotency and tumorigenesis. Alterations in their activity are hallmarks in developmental disorders and cancer as chromatin modifiers are one …
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