Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 219 for “"Polyclonal"”.
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Polyclonal interactions in intestinal tumorigenesis
… transformed cell. This contrasts with reports of polyclonality in a number of neoplastic lesions. In the first part of this thesis, I combine multicolour lineage tracing with a mouse model of intestinal tumorigenesis to show that a significant proportion of tumours have a polyclonal origin …
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Antibody-Mediated Modulation of Polyclonal Immune Responses
… how antibodies can modulate the development of a polyclonal immune response. Infusion of a monoclonal antibody prior to immunization with its cognate protein antigen resulted in two key observations. Firstly, antibody can increase the overall magnitude of the germinal center (GC) and plasmablast …
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Polyclonal somatic mutations in health and disease
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Competitive Enzyme Immunoassay by Polyclonal Antibodies Against Organophosphorus Pesticides
<p>Organophosphorus compounds (OPs) comprise one of the major classes of pesticides in use today because they are highly effective and less toxic. Because of their extensive use, there is a need for monitoring OPs in water and vegetables. Gas chromatography (GC) is the usual method for OPs …
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Dendritic Cell Regulation of Peripheral Tolerance in Polyclonal T Cell Repertoires
… approach, could be used to tolerize autoreactive polyclonal T cells, thereby preventing autoimmunity in mouse models. We succeeded in inducing tolerance to the myelin oligodendrocyte protein and preventing disease in the experimental autoimmune encephalomyelitis model. However no halt or delay in …
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Kinetic Analysis of High Affinity Polyclonal Anti-Fluorescyl Immunoglobulin-G Antibodies
Mathematical and experimental tools for the measurement and assessment of association and dissociation kinetics in anti-fluorescyl antibodies were developed in these studies. An analytic procedure for the characterization of heterogeneous first order kinetics was derived. This procedure was based …
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Exploring the Polyclonal Antibody Responses Induced by Klebsiella pneumoniae Derived Outer-Membrane Vesicles
… binding patterns by producing five sets of polyclonal serum using outer-membrane vesicles (OMVs) before characterising the resulting antibody response. Each set of polyclonal serum was then screened against a curated collection of K. pneumoniae isolates (the Warne collection) before …
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A Kinetic Study of Anti-VEGF-A Polyclonal Antibodies and Anti-VEGF-A ssDNA Aptamers
… perform similarly to antibodies, anti-VEGF-A polyclonal antibody and anti-VEGF-A ssDNA aptamer were evaluated against vascular endothelial growth factor A (VEGF-A) using Surface Plasmon Resonance (SPR). It was hypothesized that the anti-VEGF-A aptamer had the same, if not better, binding …
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Design and synthesis of aryl hydrocarbon receptor fusion proteins for polyclonal antibodies production and cellular delivery
… in rabbits and produced significant amount of polyclonal antibodies. In western blot analysis, the antibodies were able to the detect baculovirus expressed AHR and different recombinant proteins of the AHR. The polyclonal antibodies were also used in the gel-shift assay to show the AHR …
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Humanized Mouse with a Diverse Polyclonal B Cell Repertoire as a Model for Neutralizing Antibody Responses against EBV and HIV
Epstein-Barr virus (EBV) and human immunodeficiency virus (HIV) cause diseases of global health importance. However, it has been historically difficult to model human neutralizing B cell responses to these two viruses using small animals. I leveraged a transgenic mouse model that encodes a …
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A comparison of two methods of template amplification for next generation sequencing: Implications of polyclonal formation on DNA sequence for several cancer tissues
… of multiple DNA templates (referred to as polyclonal formation) is a primary disadvantage when preparing templates for NGS resulting in redundancies in DNA sequences or nonspecific noise in the sequencing. This study was conducted to compare DNA templates for sequencing prepared using two …
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Identification of an aqueous glue protein, SCP-2, and the development of a polyclonal antiserum against the bHLH transcription factor SGSF in Latrodectus Hesperus
… proteins were injected into rabbits and the polyclonal antiserum was collected and tested by western blot analysis to determine the specificity of the immunological reagent. Western blot analyses revealed the anti-SGSF antiserum was capable of recognizing bacterially expressed SGSF in an …
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Molecular Characterization of Citrus Tristeza Virus Isolates in Commercial Citrus Grown in Belize
… symptoms of CTV would test positive to a polyclonal (mild and severe symptoms) and a monoclonal (severe decline) antibody. Of the 81 trees tested, 56% tested positive to a polyclonal antibody G-604. None of the trees tested positive to the monoclonal antibody MCA-13. The second objective …
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Acyloxyacyl Hydrolase: Studies on Its Regulation and Function in Mus Musculus
… significantly higher levels of non-specific (polyclonal) IgM and IgG3 than did wild type mice. Anti-double-stranded DNA and anti-nucleosome IgM and IgG antibody levels were also higher in LPS-immunized AOAH-deficient mice than in wild type control mice. In addition, the partially-deacylated …
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Immunological Characterization of Membrane Skeletal Proteins in "Tetrahymena pyriformis" GL
… skeleton in "Tetrahymena". In this study, polyclonal antibodies were raised in rabbits against three membrane skeletal proteins isolated by low salt extraction of TKI cortical residues: band A and C, and a 23 kDa protein. Immunoblots were performed to characterize the number and size of …
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T-Cell Treatments For Solid and Hematological Tumors
… CAR<sup>+</sup> gamma/delta T cells with polyclonal TCRgamma/delta repertoire on CD19<sup>+</sup> artificial antigen presenting cells (aAPC). Enhanced cytolysis of CD19<sup>+</sup> leukemia was observed by CAR<sup>+</sup> gamma/delta T cells compared to CAR<sup>neg</sup> gamma/delta T …
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Search for etiology of porcine reproductive and neurologic syndrome: identification and characterization of a novel swine pestivirus
… those pigs, fulfilling Koch's postulates. Both polyclonal and monoclonal antibodies specific for Virus X were produced. The polyclonal antibody did not cross react with any known viral pathogens for swine except for ruminant pestiviruses (i.e., bovine viral diarrhea virus and border disease …
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