Global ETD Search
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Showing 1 to 13 of 13 for “"Polyadenylation sites"”.
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Prediction of mRNA polyadenylation sites in the human genome and Mathematical modeling of alternative polyadenylation
Messenger RNA (mRNA) polyadenylation plays many important roles in the cell, such as transcription termination, mRNA stability and transportation, and mRNA translation in eukaryotic cells. A large number of human and mouse genes have multiple polyadenylation sites (referred to as poly(A) sites) …
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The PNUTS-PP1 Complex Acts as an Intrinsic Barrier to Kaposi's Sarcoma Associated Herpesvirus Gene Expression and Replication
… controls elongation rates, slowing pol II after polyadenylation sites to promote termination. The Kaposi's sarcoma-associated herpesvirus (KSHV) co-opts pol II to express its genes, but little is known about its regulation of pol II elongation. I identified PNUTS as a suppressor of a KSHV …
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High-throughput sequencing of RNA 5'- and 3'-termini yields insights into viral and vertebrate gene expression
… 5' ends of influenza transcripts and alternative polyadenylation at the 3' ends of vertebrate transcripts. The 5' ends of influenza mRNAs include heterogeneous sequences derived from host RNAs. In a process termed cap snatching, the viral polymerase cleaves host RNAs ~10-13 nucleotides downstream …
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NOVEL RNA-BASED CANCER VACCINE FOR AML IMMUNOTHERAPY
… have found candidate neoepitopes within intron polyadenylation sites, retained-intron sites, and somatic mutation sites. Screen for immunogenic neoepitopes using 102 synthetic peptides was performed in ELISpot assays. Our final 10 candidates were cloned into a vaccine construct. mRNA cancer …
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HUMAN DPP4/CD26 TRANSGENIC MICE AS SURROGATE MODELS FOR MERS
… promoter, containing rabbit globin splicing and polyadenylation sites, as surrogate models for MERS-CoV infections. We showed that one lineage (line 52) of Tg mice globally expressing hDPP4 is highly susceptible to intranasal (i.n.) challenge with a high-dose (i.e., 106 50% tissue culture …
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Bioinformatics study of mammalian MRNA polydenylation
Messenger RNA polyadenylation is one of the key post-transcriptional events in mammalian cells, which have influences on many aspects of mRNA metabolism. Several human diseases have been shown to associate with abnormal polyadenylation, highlighting the importance of this process. The availability …
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Isolation, characterization and expression of a peach nuclear gene encoding a light-harvesting chlorophyll a/b-binding polypeptide of photosystem II
… acids. Transcription initiation and termination sites of Lhcb1*Pp1 mRNA were determined by S1 nuclease mapping. The cap site is located 70 bp upstream from the translation start site and 44 bp downstream from a putative TATA box. Multiple polyadenylation sites are present in the 3$\sp\prime$ …
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Functional analyses of ζ-crystallin binding to the 3'-UTR of glutaminase (GA) mRNA
… GA mRNAs are generated by the use of alternative polyadenylation sites and they are homologs of the rat kidney glutaminase (KGA) mRNA, while the 4.5 kb GA mRNA contains a different COOH-terminal coding and 3'-untranslated sequence. PCR cloning and sequencing confirmed that the 4.5 kb GA mRNA is …
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Modulation of Ago-miRNA regulatory networks by cis-sequence elements and target competition
… miRNAs in the Dicer KO cells. Other potential sites of miRNA-independent Ago interactions, such as histone transcripts and poly-A cleavage and polyadenylation sites, were also identified. One Ago CLIP dataset revealed the enrichment for a G-rich sequence motif at Ago target sites. We later …
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Regulation of p21 Function by a Sense Long Non-Coding RNA Generated by Alternative Polyadenylation
… used to control gene expression is alternative polyadenylation (APA). Approximately 70% of eukaryotic transcripts have multiple possible polyadenylation sites (PAS), allowing expression of mRNA isoforms with different 3’ untranslated regions (3’ UTR) as well as different open reading frames. …
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SR proteins in microRNA/mRNA biogenesis
… mRNA, which may affect the choice of alternative polyadenylation sites. The underlying mechanisms are being investigated by a variety of molecular methods. </p><p>In summary, I have identified a subset of miRNAs, the expression of which can be regulated by SF2/ASF in a splicing independent manner. …
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Posttranscriptional regulation with regard to cleavage factor I (CFI) associated alternative polyadenylation and methyltransferase A (MTA) assisted m6A modifications in Arabidopsis thaliana
… affecting the fate of a transcript. Alternative polyadenylation (APA) is associated with many regulatory aspects. It describes the addition of the poly(A) tail at alternative sites inside the transcript. This is achieved by the cleavage and polyadenylation machinery. Cleavage factor I (CFI) is a …
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Transcriptome activity of human cytomegalovirus (strain Merlin) in fibroblasts, epithelial cells and astrocytes
… is probable that other family members also share polyadenylation sites. Down-regulation of US12 family genes with potential roles in virus maturation and egress are consistent with the impaired release of virus to the extracellular medium from RPE and U373Mg cells. Compared to HFFF-2 cells, the …