Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Polyacrylamide hydrogels"”.
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Surface mechanics of polyacrylamide hydrogels following wear
… abrasive surface wear are measured. These polyacrylamide hydrogels – cross-linked polymers consisting of 92% water by mass – undergo surface wear, and their surface elastic properties before and after wear are measured. Quasi-static nanoindentations are performed on the surface using a …
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Mechanical reinforcement of polyacrylamide hydrogels using pristine single-walled carbon nanotubes
<p>Hydrogels have great promise as an innovative biomedical material possessing many advantages such as high water content, porous structure, and excellent biocompatibility. Although hydrogels have been used to develop some successful applications, they commonly do not have sufficient mechanical …
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Proliferation and morphology of pre-osteoblast cells on 25 – 1000 micron diameter patterned features on polyacrylamide hydrogels
Bone formation, also known as osteogenesis, occurs in three distinct phases: proliferation, during which cells multiply, form nodules, and begin expressing early stage osteogenic markers; extracellular matrix development and maturation, during which mature cells in nodules build a collagen matrix …
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Applications of Traction Force Microscopy in Measuring Adhesion Molecule Dependent Cell Contractility
This work describes the use of polyacrylamide hydrogels as controlled elastic modulus substrates for single cell traction force microscopy studies. The first section describes the use of EDC/NHS chemistry to convalently link microbeads to the hydrogel matrix for the purpose of performing long-term …
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Fabrication and characterization of co-continuous elastomer-hydrogel composites
… with interconnected microchannels is filled with polyacrylamide hydrogels via a microsphere-templating process. The configuration of the microstructure of the composite is observed and analyzed. Besides, the mechanical properties and diffusivity properties of the composite are experimentally …
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Engineering hydrogel environments to direct cell fate
The advent of hydrogels for biological applications has sparked interest in the both the medical and engineering community in recent years as a means to investigate cellular level processes. An area that has shown promising application of hydrogel materials has been in the study of stem cell …
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Biomolecularly and mechanically instructive materials for guiding cell-substrate interactions
… collagen-glycosaminoglycan membranes and polyacrylamide hydrogels, to evaluate stem cell diffferentiation. In summary, we have developed a photoreactive platform that provides the capability to control biomolecule density separately from mechanical properties. This strategy can be used on …
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Rheological measures of the fracture toughness of hydrogels
Hydrogels are pertinent to many applications, including tissue engineering, contact lenses and drug delivery. However, traditional tensile tests cannot be performed on soft hydrogels due to the soft and sometimes transient nature of their stiffness response. To address this issue, a method is …
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Upstream Migration Under Shear Flow: Force Generation and Molecular Regulation
… progenitor cells on ICAM-1-functionalized polyacrylamide hydrogels under physiological shear flow, an integrated platform was developed for traction force measurement during sustained upstream migration. Upstream-migrating cells generate approximately two-fold higher average and peak …
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The role of the mechanical microenvironment in regulation of cholangiocyte specification and tubulogenesis
… I built upon our previously established polyacrylamide hydrogels (Labouesse et al. 2021) alongside our 3D culture system (Mulas et al. 2020) to develop a tuneable 3D hydrogel, allowing for adjustment of ECM stiffness and composition. I then employed this system to study bile duct …
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Fluid-Driven Fractures in Elastic Hydrogels: Propagation and Coalescence
… in this research, including how to synthesise polyacrylamide hydrogels to study the processes linked with fluid-driven fracturing. These transparent, linearly elastic and brittle gels permit fracturing at low pressures and speeds allowing accurate measurements to be obtained. The broad range of …
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Polymers as directing agents for motions of chemical and biological species
… were dissolved in chloroform. Thus, 3D porous polyacrylamide hydrogels have been fabricated. The various pore sizes at the 3D porous surface have been obtained by using PS colloids with the colloid diameters ranging from 450 nm to 4000 nm. Human corneal epithelial cell growth, morphology change …
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Complex fluid lubrication mechanics of polyacrylamide surfaces
Hydrogels are used to mimic the slippery nature of biological interfaces. They provide a controlled material property, which helps to connect the properties to lubricating ability. While most hydrogel lubrication studies assume a steady state of sliding, a recent study discovered that under …
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Substrate stiffness tunes the neuronal response to the chemical guidance cue semaphorin3A
… to chemical signals. I therefore used compliant polyacrylamide hydrogels of defined stiffness to control the mechanical environment of RGCs in vitro, and studied if the mechanical environment of neurons impacts their response to Semaphorin 3A (Sema3A), a highly conserved guidance molecule that …
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Development of single-cell Raman spectroscopy for noninvasively screening the differentiation of living stem and progenitor cells in biomaterial platforms for 2D and 3D cell culture
… into damaged tissues with improved retention. Hydrogels are one of the most studied biomaterials for this purpose. They can mimic the natural extracellular matrix (ECM) to provide a protective barrier as the cells migrate to target sites, avoiding harsh conditions during transport. To develop …
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Softmatter tribology: Understanding the microstructure to property relationships of hydrogel-like materials
… on multiple other biphasic, non-biological hydrogels as well, where a prominent effect of the interfacial microstructure is observed on their mechanical and tribological properties. Yet, not only there is a lack of knowledge but also wide discrepancy about the fundamental underlying …