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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 202 for “"Polyacrylamide gel"”.
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Transverse Isotachophoresis Using Polyacrylamide Gel Electrodes
… glass chip is used with Poly(Acrylamide) gel electrodes to establish an electric field perpendicular to the direction of sample flow, causing a controlled migration of charged particles. The design and fabrication of the microfluidic chip are addressed, along with the development of a …
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Disc electrophoresis of turkey serum alkaline phosphatase isoenzymes using polyacrylamide gel
Thesis (M.S.)--Michigan State University. Dept. of Poultry Science, 1974
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Detection of Unique Tumor-Associated Proteins in the Urine of Patients with Transitional Cell Carcinoma by High Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis
<p>A two-dimensional polyacrylamide gel electrophoretic technique was developed for the detection of unique tumor-associated proteins in urine of patients with transitional cell carcinoma (TCC) of the urinary bladder. This technique yielded high resolution of polypeptides in the urine of cancer …
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Green Pea Peroxidases: Their Catalytic Properties and Thermal Stabilities
… thin-layer isoelectric focusing (IEF) on polyacrylamide gel (PAG) plates. A total of 18 (pI's 3.5-9.1) peroxidase isoenzymes were identified from crude extracts of pea seeds; all
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Experiments, Modelling, and Simulations for a Gel Bonded to a Rigid Substrate
… this thesis is to study debonding of a thin film gel induced by stress concentration on the interface with a rigid substrate, which is useful in medical device design. Using polyacrylamide gel as a model system, the configuration of a thin rectangular polyacrylamide gel covalently bonded on the …
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Isolation and Characterization of Blood Lipoproteins From Dairy Cows Fed Control or High Grain Diets (Lactation, Feeding, Lipids)
… using either preparative ultracentrifugation or gel filtration chromatography. Lipoprotein classes obtained by ultracentrifugation were very low density plus chylomicrons, low density, high density(,1), and high density. These lipoprotein classes were applied individually to an agarose gel column …
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Assay and Purification of Coxsackievirus B3
… centrifugation. Virus purity was assessed by SDS-polyacrylamide gel electrophoresis. Findings: The plaque assay for CB3 was found to be reproducible and quantitative. The dose response curve of the assay was linear between 5 and 800 plaques on a 55 cm2 tissue culture dish. Maximum yields of CB3 …
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Implementation of nano-liquid chromatography hyphenated to tandem mass spectrometry for protein identification in gel and non-gel based proteomics
… of cytosolic proteins by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. First, the techniques are highlighted that were used to identify gel separated proteins, i.e. the implementation of nano-liquid chromatography and mass spectrometry (Chapter IV). The next two …
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Isolation and Characterization of Chitinases From Dasypus Novemcinctus, the Nine-Banded Armadillo
… chitinase was purified five-fold. A 10% SDS-polyacrylamide gel electrophoresis was done with the samples from the purification procedure to visualize purification. Samples from the small intestine, proximal large intestine, and distal large intestine were aseptically collected from armadillos …
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An electrophoretic method for the isolation of isohistones from the embryo of the sea urchin Parechinus Angulosus
… urchin embryo histone variants by preparative polyacrylamide gel electrophoresis and to prove me identity of the isolated proteins by amino acid composition and partial sequencing. This serves two purposes, namely, the unequivocal identification of chromosomal proteins characterized only by …
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Growth regulation in the protozoan parasite Trypanosoma brucei: Evidence of tyrosine-specific protein kinase activity
… proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and incubated with an anti-phosphotyrosine monoclonal antibody revealed the presence of a tyrosine phosphoprotein. An alkali digestion of ($\sp{32}$P) -labeled trypanosome proteins separated by sodium dodecyl …
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Growth and Characterization of Cell Cultures of Rainbow Trout
… by molecular weight determination using SDS-polyacrylamide gel electrophoresis.
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Regulation of Adenylate Kinase
… gave a single band on a sodium dodecyl sulfate-polyacrylamide gel with M(,r) = 34,000. The interaction of this protein with adenylate kinase explains why the thermolability of the mutant adenylate kinase changed during purification and the dependence of the thermolability on concentration.
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Studies on aspects of the physiology and pathophysiology of pancreatic polypeptide
… were assessed in healthy subjects; secondly polyacrylamide gel electrophoresis was used to investigate molecular heterogeneity of the peptide; thirdly, the organ uptake and half-life time of endogenous PP was measured in pigs; and finally, basal and stimulated serum hPP concentrations were …
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Purification of glycoproteins from herpes simplex virus
… products by analytical sodium dodecyl sulphate polyacrylamide gel electrophoresis (SOS-PAGE) and autoradiography revealed that in the HSV-1 infected cells the radiolabelled components were incorporated into viral specific proteins only, whereas in the HSV-2 infected cells they were incorporated …
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ΑΠΟΜΟΝΩΣΗ ΚΑΙ ΧΑΡΑΚΤΗΡΙΣΜΟΣ ΤΗΣ ΚΙΝΑΣΗΣ ΚΑΖΕΙΝΗΣ ΙΙ ΑΠΟ ΣΠΛΗΝΑ ΠΑΙΔΙΩΝ ΜΕ ΜΕΣΟΓΕΙΑΚΗ ΑΝΑΙΜΙΑ
… BETA-THALASSEMIA MAJOR AND WAS CHARACTERIZED SDS POLYACRYLAMIDE GEL ELECTROPHORESIS AND ISOELECTRIC FOCUSING HAVE PROVED THAT THE ENZYME WAS HIGHLY PURIFIED. THE PURIFICATION STEPS INCLUDED ION EXCHANGE CHROMATOGRAPHY, AMMONIUM SULFATE FRACTIONATION AND MOLECULAR SIEVING. THE PURIFIED ENZYME …
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On-chip multi-dimensional biomolecule separation using multi-layer microfabricated valves
… of traditional two dimensional (2D) protein gel electrophoresis. Methods are described to achieve multi-dimensional biomolecule separation in a microfluidic channel. The new methods couple isoelectric focusing (IEF) with high ionic strength capillary electrophoresis (CE) by active micro valve …
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The Purification, Properties and Subunit Structure of Glycerol Dehydrogenase
… ion-exchange experiments with the native enzyme. Polyacrylamide gel electrophoresis of the enzyme treated with dithiothreitol and urea indicated the presence of two species of protein. The amino acid composition of these two proteins and of the thirteen major peaks are consistent with the …
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The chemotaxonomy of the fungal genus Endothia fr.
… criteria used by Barr were reinvestigated. Polyacrylamide gel electrophoresis and fungicide sensitivity assays were also used to examine biochemical relationships among the organisms and to establish additional means of distinguishing among the closely related taxa. The morphological …
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ΣΤΕΛΕΧΗ STAPHILOCOCCUS AUREUS ΑΝΘΕΚΤΙΚΑ ΚΑΙ ΕΥΑΙΣΘΗΤΑ ΣΤΗ METHICILLIN: ΛΥΣΙΤΥΠΙΑ, ΑΝΙΧΝΕΥΣΗ ΠΛΑΣΜΙΔΙΩΝ ΚΑΙ ΠΡΩΤΕΙΝΩΝ ΚΥΤΤΑΡΙΚΗΣ ΜΕΜΒΡΑΝΗΣ
… DIFFERENT STRAINS. MEMBRANE- PROTEIN PROFILES BY POLYACRYLAMIDE GEL ELECTROPHORESIS SHOWED THAT LMRSA STRAINS COULD BELONG TO ONE GROUP.
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