Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 133 for “"Poly A"”.
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Form and function of Poly(A) tails
Central to mRNA metabolism is the poly(A)-tail, a stretch of adenosine nucleotides at the mRNA 3' end. In this dissertation, I investigate the role of the tail in the dynamics of mRNA decay, and describe the predominant mechanisms of decay for thousands of mammalian mRNAs. Next, I examine the …
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Function and regulation of poly(A)-tail length
Poly(A) tails are found at the 3' ends of nearly all eukaryotic messenger RNAs (mRNAs) and long non-coding RNAs. The presence of a poly(A) tail promotes translation and inhibits decay of an mRNA, with both effects mediated through poly(A)-binding protein. However, an understanding of the …
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Regulatory consequences of mRNA Poly(A)-Tail length changes
… have a cap structure at their 5' ends and a poly(A) tail at their 3' ends, and the proteins that bind these features increase the stability and translation of an mRNA. The influence of the poly(A) tail on translation was discovered decades ago, but primarily with regard to the idea that an …
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The Role of the Human Nuclear Poly(A) Binding Protein in RNA Decay
… Here, I describe our work showing that polyadenosine (poly(A)) tails can stimulate transcript decay in the nucleus of human cells, a function mediated by the ubiquitous nuclear poly(A) binding protein PABPN1. We show that PABPN1 is required for the degradation of a viral nuclear …
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Functional specialization of Arabidopsis poly(A) polymerases in relation to flowering time and stress
Polyadenylation is a decisive 3’ end processing step during the maturation of pre-mRNAs. The length of the poly(A) tail has an impact on mRNA stability, localization and translatability. Accordingly, many eukaryotic organisms encode several copies of canonical poly(A) polymerases (cPAPs). The …
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Poly(A) Polymerase 1 (PAPS1) influences organ size and pathogen response in Arabidopsis thaliana
Polyadenylation of pre-mRNAs is critical for efficient nuclear export, stability, and translation of the mature mRNAs, and thus for gene expression. The bulk of pre-mRNAs are processed by canonical nuclear poly(A) polymerase (PAPS). Both vertebrate and higher-plant genomes encode more than one …
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Utilization of Poly(A)-Binding Protein in Monitoring the Formation of mRNP Granules in Toxoplasma gondii
… complex (mRNPs). I chose to study the poly(A) binding protein (PABP). Metazoans usually encode multiple isoforms of PABP to function in nucleus and/or cytoplasm but in Toxoplasma there is only one predicted PABP. In this thesis, I studied the localization of the only predicted PABP in …
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Post-transcriptional regulation of poly(A) binding proteins direct multiple facets of cardiac development and hypertrophy
… mechanisms are mediated through multiple poly(A) binding proteins—PABPC1 and PABPN1—at the 3’UTR and poly(A) tail of cardiac mRNAs to tune transcript specific and overall translation rates within the heart. These findings define a new paradigm for cardiac gene regulation with potential for …
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Analysis of the role of poly(A) -binding protein (PAB1) in the mRNA degradation process in yeast
… have also established that PAB1 contacts to the poly (A) tail made by the RRM2 domain are critical to stabilizing the CCR4-NOT complex and promoting deadenylation. The C-terminal globular domain of PAB1 through its contacts to eRF3 is also required for CCR4 deadenylation. In contrast, the RRM3 …
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Poly(A)+ RNA From Sheep Omasal Epithelium Induces Expression of a peptide Transport Protein(S) in Xenopus laevis Oocytes
… di- to octapeptide transport capability, poly(A)+ RNA isolated from sheep omasal epithelium was injected into Xenopus laevis oocytes. Poly(A)+ RNA was functionally expressed in Xenopus oocytes 4 to 7 d post-injection. Peptide (5 di-, 10 tri-, 6 tetra-, 2 penta-, 1 hepta-, 1 septa-, 1 …
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FUNCTIONAL ANALYSIS OF THE UPSTREAM PSEUDOKNOT DOMAIN AND ITS SUBSTITUTION WITH A POLY(A) TRACT IN TOBACCO MOSAIC VIRUS (TMV)
… MUTANT TMV-43A IN WHICH AN INTRODUCED 43 NT POLY(A) TRACT REPLACED THE UPD IN TMV GENOME. TMV-43A DISPLAYED REDUCED REPLICATION EFFICIENCY AND INDUCED MOSAIC SYMPTOMS IN NICOTIANA BENTHAMIANA. DEEP SEQUENCING RESULTS SHOWED THAT SMALL RNA PROFILES DIFFERED BETWEEN TMV- AND TMV-43A-INFECTED …
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Transcriptional elongation defects enhance upstream poly (A) site utilization and SPT5 affects mRNA degradation through its physical and functional interaction with CCR4 -NOT complex
… several genes, each of which contain internal poly (A) sites, whose full-length mRNA formation is reduced by mutations in RNA polymerase II subunit RPB2, elongation factor SPT5, or TFIIS. RPB2 and SPT5 defects also promoted the utilization of upstream poly (A) sites for genes that contain …
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Structural and Biochemical Investigation of the Pan2-Pan3 Deadenylase Complex
Almost all mature eukaryotic mRNAs contain a 3′ polyadenosine (poly(A)) tail, which promotes nuclear export of mRNAs, protects transcripts from exonucleolytic decay, and increases translation efficiency. As the poly(A) tail is involved in many steps of gene expression, its length is highly …
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Architecture of eukaryotic mRNA 3' end processing machinery and insights into the mechanism of polyadenylation
… all eukaryotic messenger RNAs (mRNAs) have a polyadenosine (polyA) tail at their 3′ end that is added by a multi-protein complex known as cleavage and polyadenylation factor (CPF). CPF, along with accessory cleavage factors (CF) IA and IB, cleaves the pre-mRNA within the 3' untranslated region …
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Prediction of mRNA polyadenylation sites in the human genome and Mathematical modeling of alternative polyadenylation
Messenger RNA (mRNA) polyadenylation plays many important roles in the cell, such as transcription termination, mRNA stability and transportation, and mRNA translation in eukaryotic cells. A large number of human and mouse genes have multiple polyadenylation sites (referred to as poly(A) sites) …
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Mammalian gene regulation through the 3' UTR
… (miRNA) complementary sites and cleavage and polyadenylation (poly(A)) signals, and provide evidence that specific sequence contextual features are important for their recognition. MiRNAs are 22 nt, non-coding RNAs that function as post-transcriptional gene regulators in animals and plants. …
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Régulation du transcriptome codant et non-codant chez Schizosaccharomyces pombe: facteurs et mécanismes impliqués dans la maturation 3’ des ARNs et la terminaison de la transcription
… qui inclut notamment l’ajout d’une queue poly(A) à son extrémité 3’. Au noyau, la queue poly(A) des ARNms est liée par PABPN1 (poly(A)-binding protein nuclear 1). PABPN1 fut notamment caractérisée, d’après des études in vitro, pour stimuler la réaction de polyadénylation en plus de …
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Evidence for post-transcriptional regulation of induction of NADP- specific glutamate dehydrogenase by accumulation of its mRNA in uninduced synchronous Chlorella cells
… of a high yield of total undegraded cellular polysomes was developed. The crosslinking reagent, dimethyl suberimidate, was employed to prepare a stable NADP-GDH-crosslinked-Sepharose-4B antigen affinity column for the purification of rabbit anti-NADP-GDH IgG. Binding studies with ¹²⁵I-labelled …
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A bioinformatics framework for RNA structure mining, motif discovery and polyadenylation analysis
… Further, the role played by structure in mRNA polyadenylation was investigated. Polyadenylation is an important step towards the maturation of almost all cellular mRNAs in eukaryotes. Studies have identified several cis-elements besides the widely known polyadenylation signal (PAS) element …
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