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Showing 1 to 8 of 8 for “"Polar body"”.
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Polar Body Membrane Breakdown Occurs Independent of Macroautophagy in C. Elegans
… studied the clearance of the <em>C. elegans</em> polar body during embryonic development. ATG16L1 has separable roles in regulating LC3 recruitment during macroautophagy and LAP. We demonstrated that the ATG16L1 <em>C. elegans</em> orthologs, ATG-16.1 and ATG-16.2, function redundantly to promote …
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Role of Xenopus p21-activated kinase 2 (X-PAK2) in polar body formation during oocyte maturation
Vertebrate oocytes are physiologically arrested in meiotic prophase until shortly before ovulation. Under the influence of gonadotropic hormones, these oocytes resume meiosis and progress to metaphase II, in which they await fertilization. The transition from prophase to metaphase II is termed …
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Triploid induction in the South African abalone, Haliotis midae
… South African abalone, Haliotis midae. From the polar body counts at 17 °C the release times of polar body 1 and 2 were found to be at 12 - 15 min. post insemination (pi.) and 33-36 min. pi. respectively. Cytochalasin B (CB) (0.5mg. 1-¹ seawater) and elevated temperature (30 °C) were used as …
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Production of Triploid Rainbow Trout for Evaluation in South Dakota Waters
… heat shocks to prevent expulsion of the second polar body, theoretically resulting in triploid embryos. Heat shocks were at 26 and 28°c, began 10, 20, 30, or 40 min after fertilization, and lasted for 10 or 20 min for a total of 16 treatments. Another 25,000 eggs served as controls and were …
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Impacts of perfluorooctanoic acid on porcine oocyte health and competence
… maturation was assessed by the extrusion of a polar body. Our data demonstrates that PFOA impacts the abundance of SOD1, AIF, and BAX, and addition of 40 mg/mL albumin decreases abundances of these proteins while co-exposure affects protein expression differently than PFOA alone. Interestingly, …
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Fluorescence in Situ Hybridization Analysis of Human Embryos Derived From in Vitro and in Vivo Matured Oocytes
… from germinal vesicle breakdown through first polar body extrusion. If inseminated, many in vitro matured oocytes fertilize and appear to develop normally. However, compared to embryos derived from mature oocytes, embryos derived from Prophase I oocytes produce significantly fewer pregnancies …
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In vitro maturation and fertilization of porcine oocytes and evaluation of male fertility
… to metaphase II with the extrusion of first polar body. Size of cumulus mass, time delay for oocyte selection, and degree of cumulus expansion affected oocyte maturation. Different incubation temperatures (39$\sp\circ$C, 38$\sp\circ$C, and 37$\sp\circ$C) did not affect the oocyte maturation …
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Osmotic balance and establishment of polarity in C. elegans embryo require cytochrome P450 CYP31A
… it is caused by problems in establishment of polarity and failure in the extrusion of a polar body. Moreover, we found that embryos depleted of CYP31A are osmotic sensitive and their eggs are permeable to dyes (hoechst, FM 4-64 etc.). The defects described above are common to a class of …