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Showing 1 to 7 of 7 for “"Pol IV"”.
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Molecular Genetic Analysis of Non-Catalytic Pol IV and V Subunits
… the distinction of encoding multisubunit RNA polymerases used exclusively for RNA directed DNA Methylation: RdDM) in addition to Pol I, II, and III. In Arabidopsis thaliana, Pol IV is required for the biogenesis of 24nt siRNAs whereas Pol V transcription is needed for cytosine methylation of …
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Genetic and Biochemical Properties of Arabidopsis RNA Polymerases IV and V
RNA Polymerases IV and V: Pol IV and Pol V) are plant-specific enzyme complexes with subunit homology to RNA Polymerase II: Pol II). The largest subunits in Pol IV and Pol V, NRPD1 and NRPE1 respectively, share a second largest subunit, NRPD2/NRPE2. The evolutionarily conserved Metal A and Metal B …
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Subunit Compositions of Arabidopsis DNA-Dependent RNA Polymerases and the Roles of the Plant-Specific RNA Polymerases IV and V in Gene Silencing
In addition to RNA polymerases I, II and III, the essential RNA polymerases present in all eukaryotes, plants have two additional nuclear RNA polymerases, abbreviated as Pol IV and Pol V, that play non-redundant roles in siRNA-directed DNA methylation and gene silencing. Using a combination of …
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An Investigation into the Evolution and Function of RNA-Directed DNA Methylation and its Component Proteins
… are still being characterized in land plants. Pol IV and Pol V form a major part of the RdDM process, and the subunits that compose these polymerases have evolved independently across the land plant lineage, resembling a stepwise manner of evolution. While a Pol IV/Pol V-like first subunit …
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Single-molecule studies of bacterial DNA replication and translesion synthesis
… of genomic DNA is crucial for the survival of a cell. In order to achieve high-level accuracy in copying its genome, all cells employ replicative DNA polymerases that have intrinsic high fidelity. When an error occurs on the template DNA strand, in the form of lesions caused by diverse …
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A novel role for the transcriptional modulator NusA in DNA repair/damage tolerance pathways in Escherichia coli
… damage tolerance are crucial for cellular survival after DNA damage. Translesion DNA synthesis (TLS) is one such mechanism of DNA damage tolerance which utilizes a specialized translesion DNA polymerase capable of catalyzing DNA synthesis on imperfect templates. There are two TLS polymerases …
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Small RNAs within the developing seed of Arabidopsis thaliana
… range of dynamic epigenetic phenomena, but relatively little is known about the small RNA (sRNA) populations found within the tissues composing the seed. We set out to profile the sRNA complement found within whole seeds and the embryo and endosperm tissues within the seed. In whole seeds, …