Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 56 for “"Pol II"”.
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DNA topology and Pol II CTD phosphorylation as stepwise regulators of chromatin architecture in differentiating human stem cells
… lineages. This commitment is mediated by RNA polymerase II (Pol II), which is recruited by transcription factors, guided by enhancers, excluded from heterochromatin, and repositioned through DNA looping. While each of these features of Pol II regulation has been well studied in individual cell …
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Structural and biochemical characterization of RNA polymerase II transcription
… One such mechanism involves pausing of RNA polymerase II (Pol II) in the promoter-proximal region of genes. Pausing is stabilized by the protein complexes DRB-sensitivity inducing factor (DSIF) and negative elongation factor (NELF). Prior structural and biochemical studies provide specific …
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An Alternative View of the Templates and Functions of RNA Polymerase II
<p>RNA polymerase II (pol II) has been implicated in the RNA-templated RNA synthesis during replication of some Viroids and Hepatitis Delta Virus (HDV). In this study HDV RNA-templated pol II transcription was examined in vitro, using HeLa cell nuclear extracts (NE). Under standard conditions for …
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The PNUTS-PP1 Complex Acts as an Intrinsic Barrier to Kaposi's Sarcoma Associated Herpesvirus Gene Expression and Replication
Control of RNA Polymerase II (pol II) elongation is a critical component of gene expression in mammalian cells. The PNUTS-PP1 complex controls elongation rates, slowing pol II after polyadenylation sites to promote termination. The Kaposi's sarcoma-associated herpesvirus (KSHV) co-opts pol II to …
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Changes in the Rpb3 Interactome Caused by the Deletion of RPB9 in Saccharomyces cerevisiae
RNA Polymerase II (Pol II) is the primary actor in the transcription of mRNA from genes. Pol II is a complex composed of twelve protein subunits. This study focused on the changes in the interactome of Rbp3 in S. cerevisiae when the Pol II subunit Rpb9 is removed. Rpb3 is one of the core subunits …
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Regulation of Gene Expression in Trypanosoma brucei
… eukaryote, in T. brucei genes transcribed by RNA polymerase II (RNA pol II) are arranged in polycistronic transcription units (PTUs). mRNAs are separated post-transcriptionally by coupled splicing and polyadenylation reactions. During the splicing reaction a 39 nt 'sliced-leader' is added to every …
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Regulation of Transcription Through RNA Polymerase II Promoter-Proximal Pausing
… Transcription is carried out by the enzyme RNA Polymerase II (Pol II). Regulation of RNA Pol II occurs at every step of transcription, the most well studied one being transcription initiation. However, in many metazoan genes, after transcription initiates, RNA Pol II experiences a pause 20-60 …
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RNA Polymerase II control in the changing transcriptional landscape
Eukaryotic RNA polymerase II (Pol II) is a 12 subunit complex responsible for transcribing all protein coding genes, as well as producing many other RNA species. Regulation of gene expression is essential for the viability of any organism, and it is primarily achieved at the transcriptional level. …
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On co-transcriptional splicing and U6 snRNA biogenesis
Messenger RNA (mRNA) is transcribed by RNA polymerase II (Pol II) and has to undergo multiple processing events before it can be translated into a protein: a cap structure is added to its 5’ end, noncoding, intervening sequences (introns) are removed and coding exons are ligated together and a …
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Subunit Compositions of Arabidopsis DNA-Dependent RNA Polymerases and the Roles of the Plant-Specific RNA Polymerases IV and V in Gene Silencing
In addition to RNA polymerases I, II and III, the essential RNA polymerases present in all eukaryotes, plants have two additional nuclear RNA polymerases, abbreviated as Pol IV and Pol V, that play non-redundant roles in siRNA-directed DNA methylation and gene silencing. Using a combination of …
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AN INVESTIGATION OF THE MECHANISMS OF GROUCHO MEDIATED TRANSCRIPTIONAL REPRESSION
… mediate transcriptional repression by promoting Pol II promoter-proximal pausing. In this work, I took three different approaches to test this model, and gain understanding of Gro-mediated repression. For the first approach, genetic assays exploiting the GAL4/UAS system were used to drive the …
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Defining the molecular basis for the β-catenin and CDC73 interaction
RNA polymerase II (Pol II) is required for expression of protein coding genes. To initiate gene transcription, Pol II is recruited to gene promoters by transcription factors. Gene specific transcription factors, including β-catenin, a component of the Wnt signaling pathway, are used to regulate …
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c-Myc regulates transcriptional pause release and is a global amplifier of transcription
… evidence that c-Myc is a global regulator of RNA Polymerase II (RNA Pol II) transcriptional pause release. Transcriptional pausing occurs when additional regulatory steps are required to promote elongation of genes after transcription has initiated. Chapter 2 identifies transcriptional pausing as …
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STAT3-protein interactions in IL-6/gp130 signaling
… is accompanied by increased loading of RNA Pol II and phospho-Ser2 CTD Pol II on the ã-FBG TATA box and coding regions. Finally, both IL-6-inducible RNA Pol II and phospho-S2 CTD RNA Pol II association with the endogenous ã-FBG gene are significantly decreased when CDK9 kinase activity is …
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Enzymatic Disassembly of Promoter Bound 7SK snRNP Drives Transcription Elongation of HIV and Cellular Genes
… Shortly after transcription initiation, RNA Polymerase II (Pol II) pauses and it only enters into productive elongation after inducible transcription factors (TF’s) recruit the P-TEFb kinase to phosphorylate Pol II in response to stimuli. To ensure tight regulation of this process, the …
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Co-transcriptional recruitment of the U1 snRNP
… with respect to the recent proposal that RNA polymerase II (Pol II) recruits pre-mRNA splicing factors to active genes. In my thesis, I address: 1) whether the U1 snRNP, which binds to the 5¡¦ splice site of each intron, is recruited co-transcriptionally in vivo and 2) if so, where along the …
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Investigating the interactions of G-quadruplexes with transcription and chromatin structure
… G4s (~20%), which accompanied a reduction in RNA polymerase II (RNA Pol II) loading. This indicates that chromatin accessibility, as opposed to transcriptional activity, is a determinant of G4 formation. To assess whether G4s function to promote transcription, the small molecule ligand pyPDS was …
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PolyA signals located near 5’ of genes are silenced by a general mechanism that prevents premature 3’ end processing
PolyA signals located at the 3’ end of eukaryotic genes drive the cleavage and polyadenylation reaction to the nascent pre-mRNA. Although these sequences are expected only at the 3’ end of genes, we found that strong polyA signals are also present within the 5’ untranslated regions (UTRs) of many …
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Identification of Novel Core and Accessory Factors Involved in Nucleotide Excision Repair in Yeast
… to be triggered by a stalled elongating RNA polymerase II (Pol II) complex. Rad26, the homolog of the human CSB gene, and Rpb9, a nonessential subunit of Pol II, play important roles in TCR. We identified a dual role for PAF in TCR. In the presence of Rad26, PAF plays a positive role, …
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New Roles in RNA Stability and Transcription for the RNA Export Protein, REF/Aly
… of events including capping, splicing, and polyadenylation, which occur co-transcriptionally, are interdependent, and are coupled to other steps in gene expression including transcription and RNA export. For example, in mammalian cells the TREX (transcription-export) complex is deposited on …
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