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Showing 1 to 16 of 16 for “"Pol I"”.
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DNA structural selectivity of binding by the Pol I DNA polymerases from Escherichia coli and Thermus aquaticus
… the thermodynamics of substrate selection by DNA Polymerase I is important for characterizing the balance between replication and repair for this enzyme <I>in vivo</I>. Due to their sequence and structural similarities, Klenow and Klentaq, the “large fragments” of the Pol I DNA polymerases from …
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Mechanism and regulation of ribosomal RNA transcription
The sole responsibility of RNA polymerase I (pol I) is the transcription of the ribosomal RNA (rRNA) genes. This transcription can account for up to 60% of the total cellular RNA that is being transcribed in an actively dividing cell. Due to the large amounts of energy this transcription requires, …
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Funktionelle Charakterisierung der CK2-vermittelten Phosphorylierung des Transkriptionsfaktors TIF-IA bei der Regulation der Synthese ribosomaler RNA
In Säugern wird die ribosomale RNA von RNA-Polymerase I (Pol I) synthetisiert. Die Interaktion der Pol I-spezifischen Faktoren TIF-IA und TIF-IB miteinander sowie die Bindung von TIF-IB an den rDNA-Promotor sind für die Transkriptionsinitiation notwendig. Ein Guanin an Position -7 (G-7) im core …
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Biochemical characterization of the E. coli Very Short Patch Repair pathway and its coordination with methyltransferase repair of 0⁶-methylguanine
… VSPR. Genetic studies also indicate that Pol I is responsible for removing and replacing a short tract of nucleotides downstream of the incision site and that DNA Ligase seals the nick to complete the repair event. However, until now, biochemical investigation of the repair steps …
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Zur funktionellen Architektur des Nukleolus in lebenden Zellen
… einzelnen wurde durch die Untersuchung von RNA-Polymerase I der rDNA Transkriptionsort im fibrillären Zentrum des Nukleolus bestätigt. Die kinetischen Analysen von zwei pol I-Untereinheiten (RPA194 und RPA53) durch FRAP in transkriptionell aktiven und inaktiven Nukleoli erlaubten direkte …
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Solution Conformations and Structural Thermodynamics of Type I DNA Polymerases from Escherichia coli and Thermus aquaticus
The global conformations of DNA polymerase I from Thermus aquaticus (Taq/Klentaq) and E. coli (Pol I/Klenow) both in isolation and in complex with DNA have been examined in solution using hydrodynamic (analytical ultracentrifugation) and small/wide angle X-ray scattering techniques and then …
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Role of translesion DNA polymerases in mutagenesis and DNA damage tolerance in Pseudomonads
… replikatsiooni, takistades replikatiivse DNA polümeraasi edasiliikumist. Pikaajaline replikatsiooni seiskumine võib osutuda rakkudele letaalseks. Selleks, et DNA replikatsioon saaks jätkuda ka kahjustatud DNA-lt, on välja kujunenud DNA kahjustuste tolereerimise mehhanismid. Üheks neist on DNA …
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Molecular Genetic Analysis of Non-Catalytic Pol IV and V Subunits
… the distinction of encoding multisubunit RNA polymerases used exclusively for RNA directed DNA Methylation: RdDM) in addition to Pol I, II, and III. In Arabidopsis thaliana, Pol IV is required for the biogenesis of 24nt siRNAs whereas Pol V transcription is needed for cytosine methylation of …
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Eukaryotic Transcriptional Activation Mechanism: Protein-Protein Interactions
… those with acidic activators, TFIIB, Dr1 (NC2), Pol I- and Pol III- specific factors. These mutational analyses have provided an insight into how the interplay of many regulatory factors occurs on the surface of a target factor, TBP, to specify and regulate transcriptional activity. Furthermore, …
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An investigation of the replication of the broad-host-range plasmid pTF-FC2
… were able to replicate independently of DNA polymerase I (Pol I) in Escherichia coli and this feature was used to distinguish between replication from the T. ferrooxidans origin and the pMB1-derived origins of the vectors. A 3.2 kb Sau3A partial fragment was obtained which had retained the …
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In Vivo Studies of Ribosomal RNA Transcriptional Regulation within a Drosophila Stem Cell Lineage
As the first step of ribosome biogenesis, RNA polymerase I-directed ribosomal RNA gene (rRNA) transcription is critical for cell growth, proliferation and cell survival. Upregulated rRNA levels have been observed in many types of cancers. However, the extent to which rRNA transcription is …
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Structural and Computational Studies of RNA Polymerase
<p>Although the multi-subunit RNA Polymerase (RNAP) structures have revolutionized our understanding of transcription, we still do not fully understand the molecular details of bacterial promoter recognition and melting. In addition, our understanding is generally limited to highly conserved …
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Characterization of a novel family of nucleolus localized RNAs
… suggested that SNUL-RNA is transcribed by RNA Pol I and behaves similarly to pre-rRNA during biological processes when the nucleolar structure is disrupted. Long-read RNA-sequencing analyses indicate that full-length SNULs show significant similarities to pre-rRNAs, implying that SNULs could be …
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Purification and characterization of TFIIIA from Acanthamoeba castellanii
… has been in studying the mechanisms of RNA polymerase III (Pol III) transcription and the regulation of this process in A. castellanii, which was specifically focused on TFIIIA. This factor only activates Pol Ill-mediated transcription from 5S RNA genes and is the first of three general Pol …
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The waning of the two giants: the electoral decline of the Italian Christian Democratic Party and the Italian Communist Party
Italy is currently experiencing profound political change. One aspect of this change involves the decline in electoral support for the Italian Christian Democratic Party (DC) and the Italian Communist Party (PCI), now the Democratic Party of the Left (PDS). Signs of the electoral decline of both …