Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Pnpase"”.
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Synchrony of harmony and chaos in a dynamic, bacterial riboregulatory machine
… ribonuclease of the complex, the exoribonuclease PNPase. Finally, the last canonical component is the glycolytic enzyme enolase, for which the precise function in the context of the RNA degradosome is not fully understood. The scaffold domain of the RNA degradosome has a conserved propensity for …
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Fantastic ribonucleoprotein complexes and how to study them. Architectural principles of regulatory assemblies in bacteria.
… by repurposing the conserved ribonuclease PNPase (polynucleotide phosphorylase) into an RNA chaperone. Cryo-EM structures presented here show how the flexible KH-S1 portal of PNPase generally guides the 3ʹ-end of the sRNA sponge 3ʹETSleuZ to the catalytic core for digestion but reroutes the …
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Environmental sensing and response of conserved RNA-processing machines to specific external chemical cues
… of nearby genes. Polynucleotide phosphorylase (PNPase) is a homotrimeric protein whose primary function is to degrade and recycle mRNA; yet it also plays an important role in RNA quality control by sequestering oxidatively damaged RNAs. While much is known about the primary functions of these …
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A continuous spectrophotometric assay for phosphate releasing enzymes
… pH rate profiles have been reproduced using the PNPase/m$\sp7$Ino coupled assay. Initial velocity patterns constructed over micromolar to millimolar concentrations of aspartate and CbmP yield four kinetic parameters simultaneously. To further illustrate the application of this coupled assay, …
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Towards Understanding Helicase and Chaperone Activities in the RNA Degradosome
… enolase, and the phosphorolytic exoribonuclease PNPase. The degradosome has also been previously shown to associate with the RNA chaperone Hfq to form a small RNA guided machinery that targets defined transcripts. This thesis attempts to investigate several characteristics of the degradosome, …
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Biophysical characterization of the Escherichia coli RNA degradosome
… enzyme enolase, and the exoribonuclease PNPase. The main scaffold component of the RNA degradosome, RNase E, contains an amphipathic alpha helix that recruits the degradosome to the cytoplasmic membrane. Investigations on the membrane binding properties of the RNase E as well as the RNA …
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Analyzing the functions of human polynucleotide phosphorylase (hPNPaseold-35)
… enzyme is polynucleotide phosphorylase (PNPase). The human homologue of this fascinating enzymatic protein (hPNPaseold-35) was cloned a decade ago in the context of terminal differentiation and senescence through a novel ‘overlapping pathway screening’ approach. Since then, significant …
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Roles of non-Cas nucleases in Type III-A CRISPR-Cas immunity
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-08-01