Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 123 for “"Pluripotent stem cell"”.
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Engineering pluripotent stem cell heterogeneity and its effects on differentiation
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-05-01
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Defining the essential regulators of naive human pluripotent stem cell reprogramming
Human pluripotent stem cells (PSCs) exist in two distinct states – naïve and primed, which are interconvertible. Naïve PSCs uniquely harbour several desirable properties that primed PSCs do not, including their ability to model human pre-implantation biology and developmentally-regulated epigenetic …
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The role of mechanotransduction in signalling and pluripotent stem cell fate
… development is a dynamic process in which cells are exposed to soluble and mechanical cues in the microenvironment in a spatially and temporally organised manner. Although increasing evidence suggests that the regulation of pluripotent stem cell fate is strongly influenced by external …
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Extracellular matrix- and pluripotent stem cell-based tissue engineering of the kidney
… kidney model should emulate the architecture and cell types of the kidney, as well as the mechanical properties and composition of the extracellular matrix. In order to emulate the function of the kidney, the model must be perfused. Therefore, a scaffold-based tissue engineering approach was …
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Roles for cell surface glycans in guiding human pluripotent stem cell fate
Cell-surface polysaccharides are a fundamental component of the stem cell microenvironment. They are known to modulate developmental signals- critical for pluripotency and differentiation. Nevertheless, the architecture of the cellular glycocalyx and how these structures direct the fate of human …
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Approaches to Reduce Selection of Genomic Variants in Human Pluripotent Stem Cell Culture
… that reduce genomic instability in human pluripotent stem cells (hPSCs) is an unmet challenge in the field. Results from our lab and numerous research groups demonstrate that hPSCs are prone to genomic aberrations and single-cell passaging increases the rate of genomic alterations. …
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Generation and characterisation of primary and pluripotent stem cell-derived human hepatoblast organoids
Use of human pluripotent stem cells (hPSCs) has an unique potential for cell therapy since they could allow the production of almost any cell type at a large scale. Accordingly, many protocols have been developed to differentiate hPSCs into cells of clinical interest, including hepatocytes. …
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Investigating the effects of tau mutations in induced pluripotent stem cell-derived neurons
… In this project, I differentiated induced pluripotent stem cells (iPSCs) derived from control subjects and people with the N279K or V337M MAPT mutations to monolayer neurons. There was an increase in the proportion of neurons containing tau phosphorylated at the AT8 epitope in N279K …
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Effects of low oxygen culture on pluripotent stem cell differentiation and teratoma formation
Pluripotent stem cells (PSC) hold promise for the study of embryonic development and the treatment of many diseases. Most pluripotent cell research is performed in incubators with a gas-phase oxygen partial pressure (p02) of 142 mmHg. However, embryonic cells in early development are exposed to a …
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Multi-scale imaging and informatics pipeline for in situ pluripotent stem cell analysis
Human pluripotent stem (hPS) cells have the ability to reproduce indefinitely and differentiate into any cell type of the body, making them a potential source of cells for medical therapy and an ideal system to study fate decisions in early development. However, hPS cells exhibit a high degree of …
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CORRECTION OF THE GENETIC DEFECT IN INDUCED PLURIPOTENT STEM CELL LINES THROUGH CHROMOSOME TRANSPLANTATION
Since the discovery of stem cell in 1961, the knowledge and applications of pluripotent stem cells have expanded significantly, opening remarkable opportunities for fundamental discoveries and biomedical advancements, such as regenerative medicine, disease modelling, and drug screening. A pivotal …
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Human induced pluripotent stem cell models of Rett Syndrome reveal deficits in early cortical development
… human neurons with the use of induced pluripotent stem cells (iPSCs) derived from RTT patient skin samples. We generated a variety of monolayer and 3D neuronal models and found that RTT phenotypes are present at the earliest stages of brain development including neuroepithelial …
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The Maturation of Human Pluripotent Stem Cell-Derived Retinal Ganglion Cells and Their Degeneration in Glaucoma
… leading to the degeneration of retinal ganglion cells (RGCs) and eventual blindness. A need exists to better understand the maturation of human RGCs as well as their degeneration, with the goal of developing new therapeutics diseases like glaucoma. Human pluripotent stem cells (hPSCs) provide an …
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A Human Pluripotent Stem Cell-Derived Model for the Investigation of Thermogenic Adipocyte Development and Function
… for the in vitro differentiation of human pluripotent stem cells (hPSCs) to brown adipocytes (BAs). This thesis describes how this protocol can be employed to investigate candidate genes with proposed roles in BAT regulation. The thesis also investigates how integration of alternative cell …
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The role of Synaptic Genes NRXN1 and SHANK3 in Autism: an induced pluripotent stem cell study
… and atypical communication between brain cells. This thesis aims to first establish and characterise an induced pluripotent cell (iPSC) derived neuronal model suitable for studying autism related synaptic genes. Secondly, it aims to study the effect of pre-synaptic NRXN1 and post-synaptic …
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INDUCED PLURIPOTENT STEM CELL DERIVED LIVER MODEL FOR THE STUDY OF PNPLA3-ASSOCIATED NON-ALCOHOLIC FATTY LIVER DISEASE
… steatohepatitis (NASH), cirrhosis, and hepatocellular carcinoma. Until recently, NAFLD has been considered to be a consequence of metabolic syndrome; however, recent studies suggest that genetic factors may also influence disease onset and progression. Accordingly, genome wide association …
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The core mammalian pluripotency network in induced pluripotent stem cell (iPSC) formation : models for genetic and epigenetic reprogramming
… experiment that converted mouse fibroblasts to a pluripotent phenotype coined the 'induced pluripotent stem cell' (iPSC) state. Unhindered by ethical or immunogenic constraints, iPSCs potentially hold the keys to tremendous applications in therapeutic and regenerative medicine. Furthermore, …
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Maturation of pluripotent stem cell-derived alveolar type II cells at air-liquid interface and response to environmental stimuli
Human pulmonary alveolar type II cells (AT2s) are facultative progenitors of the distal lung epithelium and secrete surfactant, a protein-lipid mixture that lowers alveolar surface tension and prevents alveolar collapse during expiration. AT2s exist at a physiological interface between inhaled air …
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Investigating the role of a FAM111B mutation in hereditary fibrosing poikiloderma (POIKTMP) using induced pluripotent stem cell (iPSC) model
… the FAM111B mutation (c.1861T>G) in the multi-systemic fibrosis affecting the South African kindred with POIKTMP. Dermal fibroblasts from two affected siblings and a familial control were reprogrammed into induced pluripotent stem cells (iPSCs) via the Sendai virus vector (SeVdp) packaged with …
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