Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 174 for “"Pluripotency"”.
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Reprogramming to Pluripotency Using Small Molecule Compounds
<p>The generation of induced pluripotent stem cells (iPSCs) through the use of small molecule compounds has evolved as a potential cellular reprogramming strategy. Individually, specific small molecule compounds have previously been shown to replace reprogramming transcription factors or enhance …
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Pluripotency and patterning in nonhuman primate embryogenesis
… to unravel primate-specific features of pluripotency. This thesis examines common marmoset (Callithrix jacchus) development to explore the molecular cues that govern (1) naïve pluripotency in the preimplantation epiblast, and (2) primed pluripotency in the embryonic disc (EmDisc) in the …
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Induced pluripotency and reprogramming by defined factors
… to explore factors that regulate induction of pluripotency in somatic cells. Nuclear reprogramming by SCNT occurs in an 'indirect' manner by unidentified components within oocyte cytoplasm and requires the destruction of embryos. The introduction of induced pluripotent stem cells (iPS cells) …
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Chromatin Regulation in Somatic Cell Reprogramming to Pluripotency
Contains fulltext : 215040.pdf (Publisher’s version ) (Open Access)
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Cryo-electron microscopy studies of pluripotency factor Nanog
… rise to cell types from all lineages, known as pluripotency. Culture of ES cells in vitro requires addition of signalling molecules such as LIF to block differentiation signals and maintain self-renewal. At the transcriptional level, self-renewal is governed by the ‘network of pluripotency’; a …
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Regulation and patterning of cell differentiation and pluripotency
… and patterning of cell differentiation and pluripotency, by pursuing three related research projects: 1. Reversible immortalization of cardiomyocytes to enable their proliferation necessary for obtaining large cell numbers 2. Patterning of the delivery of Doxycycline (Dox), the expression …
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Reprogramming human somatic cells to pluripotency using RNA
… us to reprogram single adult fibroblasts to pluripotency with a 44% success rate (n = 9). Our results suggest that the efficiency and kinetics of reprogramming methods need not be limited by a fundamental stochastic element as has been suggested. Due to the high efficiency, speed, …
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Instructing naïve pluripotency ; JAK/STAT3 and Nanog in reprogramming
Reprogramming of somatic cells to pluripotency is a topic of extensive research driven by the potential of induced pluripotent cells in in vitro disease modelling, drug screening and regenerative medicine. The pluripotency associated transcription factor Nanog has been subject to a lot of …
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Dissecting Pluripotency and Mammalian Embryonic Development via Droplet Microfluidics
Pluripotency, the ability of a cell to differentiate towards any type of somatic cell is a transient feature of the developing embryo. In vitro, pluripotency can be captured in the form of embryonic stem cells (ESCs). In this study, I developed a microfluidic-based system to encapsulate ESCs into …
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Determining the Signalling Pathways that Govern Human Naive Pluripotency
… and occupy a developmentally advanced state of pluripotency comparable to mouse EpiSCs. Recent reports demonstrate that a naïve state of human pluripotency can be consistently derived either through transient histone deacetylase inhibition mediated resetting of conventional hESCs or via …
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Regulation of amnion induction during the human formative pluripotency transition
… amniotic ectoderm (AME) segregation and explore pluripotency transitions modulate AME competency. Single cell RNA-sequencing reveals AME specification and confirms that the in vitro derived AME cells closely resemble embryo AME rather than TE. Unexpectedly, TE potential persists during early …
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USING A NOVEL CELL- PENETRATING PEPTIDE TECHNOLOGY TO INDUCE PLURIPOTENCY
… that only four genes were required to induce pluripotency: Oct4, Sox2, Klf4, and c-Myc (OSKM). Since then, pluripotency has been induced with OSKM most effectively by transfection or transduction. However, both are difficult to control and can have undesirable off-target effects, e.g. random …
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Phenomenological models in biological physics : cell growth and pluripotency maintenance
… vitro maintenance of the embryonic stem cell's pluripotency by a complex transcriptional network.
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BMP Signaling Regulates Germ Cell Pluripotency, Sexual Differentiation, and Cancer Susceptibility
… highlight the potential links between germ cell pluripotency, sexual differentiation and cancer susceptibility. A recent GWAS study [3] identified association of BMP7 with testicular dysgenesis syndrome; however the molecular mechanisms behind this association remain unknown. Previously, we …
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Microfluidic Devices for the Investigation of Pluripotency in Embryonic Stem Cells
… if we are to understand the mechanisms of pluripotency.
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The NuRD complex regulates chromatin dynamics driving exit from naïve pluripotency
… (NuRD) complex is essential for exit from naive pluripotency both in mammalian embryonic stem cell (ESC) culture and in vivo. However, the specific mechanisms of chromatin modulation by NuRD in this process have not been fully characterised. This study used rapid, auxin-inducible removal of NuRD …
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Mechanistic Dissection of Tcf7l1-Dependent Enhancer Decommissioning During Exit from Naïve Pluripotency
The transition out of naïve pluripotency requires the simultaneous dissolution of the naïve gene regulatory network as well as a redirection of transcriptional response to Wnt/β-catenin signaling. Here, we show that Tcf7l1 functions as a linchpin factor that connects the two outcomes via effects at …
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