Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Plp2"”.
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Structural and functional studies of the papain-like protease 2 from mouse hepatitis virus
… in virus immune evasion. To this end, we chose PLP2 from mouse hepatitis virus (MHV) as our target of study because MHV has historically served as a model system for the study of CoVs, and it has undeniable advantage of ease in culturing in comparison to human coronaviruses.</p> <p>It is …
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A Study of Human Coronavirus NL63: Characterization of Virus Replication, Targeting of Papain-Like Protease 2 by Antivirals and Probing Virus Mediated Innate Immune Antagonism
… activity of the viral papain-like protease 2 (PLP2) domain. Using quantitative real time PCR and imaging techniques I showed that HCoV-NL63 undergoes productive replication in the ciliated bronchial epithelial cells. To identify potential broad spectrum inhibitors of HCoVs, small molecule …
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Multifunctional Coronavirus Papain-Like Proteases as Targets for Antiviral Therapeutics and Vaccines
… protease and deubiquitinase activity of PLP2s from HCoV-OC43, HCoV-229E, HCoV-HKU1, MHV and FIPV.</p><p>To evaluate the role of PLP during virus replication and pathogenesis I utilized two approaches and a mouse model system, mouse hepatitis virus (MHV). First, I performed deletion …
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The roles of papain-like protease-related proteins in viral replication and host immunity
… and nsp2N share the same N-terminal PLP2 domain. In chapter 2, nsp2TF and nsp2N were demonstrated to be critical for host innate immunity suppression at least through the PLP2 domain-mediated deubiquitination and deISGylation effects. The infection of nsp2TF/nsp2N knockout mutants …
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A reverse genetics study of the Human Coronavirus 229E ADP-ribose-1"-phosphatase
… pp1a/pp1ab nsp1-to-nsp4 region revealed that PLP2 is responsible for processing at the predicted nsp3|nsp4 cleavage site while PLP1 does not cleave at this site. The data provide new insight into HCoV-229E PLP substrate specificities and polyprotein processing pathways. <br/><br/>Second, a …
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Coronavirus Replicase Proteins: Multifunctional Mediators of Replication and Innate Immunity Evasion
… In contrast to MHV, the PLPs from HCoV-NL63 (PLP2) and SARS-CoV (PLpro) were able to inhibit type I IFN induction independently of catalytic activity albeit to a lesser degree than wildtype. Further investigation revealed that the PLPs associated with and inhibited the dimerization of a key …
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Molecular Basis of Mechanosensitivity
… the behavior of Piezo1 in vitro. This protein, Plp2, is a small transmembrane protein of undescribed function that amplifies the magnitude and slows down the kinetics of Piezo1 in heterologous expression. The other protein, Cd63, is also a transmembrane protein that only amplifies the magnitude …