Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"Plastid transformation"”.
-
Improved plastid transformation in Marchantia polymorpha
Plastids are subcellular organelles which perform photosynthesis in plants and algae. They are descended from free-living photosynthetic bacteria which were engulfed by early eukaryotes ~1.5 billion years ago. Nearly all plastids retain a small genome separate from the nuclear genome of the plants …
-
Towards plastid transformation in rapeseed (Brassica napus L.) and sugarbeet (Beta vulgaris L.)
… establishment of conditions that make possible plastid transformation in both species. Tobacco leaf protoplasts (cv. ”petite Havana”, cv. ”Wisconsin 38”) were used to develop a novel technique – the TAL (thin-alginate-layers) technique. The TAL technique in combination with new culture media …
-
An Investigation into Transformation and RNA Editing in the Lolium Plastome
… resulting in altered transcripts) within plastidial transcripts of the NDH complex (NADH dehydrogenase complex) in relation to the drought response of several accessions of perennial ryegrass. Previous studies have shown that the NDH complex is involved with countering oxidative stress …
-
Development and application of novel genetic transformation technologies in maize (Zea mays L.)
… flow via pollination. In most crops, the plastid genome is inherited uniparentally in a maternal manner. Consequently, a trait introduced into the plastid genome would not be transferred to the sexually compatible relatives of the crops via pollination. Thus, beside its several other …
-
An investigation into the chloroplast transformation of wheat, and the use of a cyanobacterial CCM gene for improving photosynthesis in a C3 plant
… improvement has many potential advantages over transformation of the nuclear genome. The genetic modification of the chloroplast genome via transformation was first demonstrated in the late 1980’s, and since then, chloroplast transformation of many Dicotyledonous (dicot) plant species such as …
-
The complete plastid genome sequence of Passiflora cincinnata: genome rearrangements, massive plastid gene losses and implications to genome-plastome incompatibility
The plastid genome (plastome) organization, gene content and order is well conserved in most angiosperms, but there are some exceptions. The Passiflora genus is one of those exceptions, because there are evidences of some unusual plastid gene losses to species of this genus. However, none plastome …
-
Chloroplasts as bioreactors : high-yield production of active bacteriolytic protein antibiotics
… retarded growth, presumably due to exhaustion of plastid protein synthesis capacity, development and seed production were not impaired under greenhouse conditions. Since Pal and Cpl-1 showed toxic effects when expressed in E. coli, a special plastid transformation vector (pTox) was constructed to …
-
Functional analysis of plastid-encoded genes
Plastid chromosomes from the variety of plant species contain several conserved open reading frames of unknown function, which most probably represent functional genes. The primary aim of this thesis was the analysis of the role of two such ORFs, designated ycfs or hypothetical chloroplast reading …
-
Analysis of cis-acting expression determinants of the tobacco psbA 5’UTR in vivo
… regulatory factors. In addition, a versatile plastid transformation vector pKCZ with an insertion site in the inverted repeat region of the plastid genome was constructed. In all constructs, the psbA 5’UTR (Wt or modified) was used as the 5’ leader of the reporter gene uidA under control of …
-
Development of a plant-based vaccine against malaria
… lines have been generated using biolistic transformation, and these are confirmed to carry the synthetic gene construct. The synthetic GK1 peptide is confirmed to be expressed using RT-PCR and Western blots, and detected by RP-HPLC at levels of up to 6 µg g-1 dry weight of tobacco leaf …