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Showing 1 to 20 of 409 for “"Plasmids"”.
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Characterization of plasmids in Gluconobacter
… The only other known report of gluconobacter plasmids was by Fukaya, et al. who reported that 23 of 36 strains examined contained plasmids. I wish to learn more about the presence and significance of plasmids in the gluconobacters. In this study, I selected 14 strains representing the three …
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Analysis of catabolic plasmids in pseudomonas
The experimental work carried out for this thesis concentrated on two separate aspects of the molecular biology of toluenedegradative (Tot) strains of Pseudomonas. Pseudomonas strain Mr74 harbours the single TOt plasmid pWW74, and this plasmid carries two related genes for catechol 2,3-oxygenase …
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Charakterisierung des Replikationsproteins ORF904 des archaealen Plasmids pRN1
Das Plasmid pRN1 kann als Modell für die Untersuchung der DNA-Replikation im thermoacidophilen Crenarchaeon Sulfolobus genutzt werden. Ein offenes Leseraster kodiert für das Protein ORF904. Es konnte bereits gezeigt werden, dass dieses Protein eine N-terminal lokalisierte …
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RNA-based portable genetic circuits and synthetic plasmids
The lack of modularity impedes the creation of synthetic biological systems at the same level of complexity as modern electronic systems, which can contain millions of logic gates. Recently, the development of novel RNA regulators poses an opportunity to overcome this challenge. In this thesis …
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The Role of Plasmids in Microbial Secondary Metabolism
Pseudomonas tabaci, P. angulata and P. coronafaciens are three closely related plant pathogens that differ only in their ability to produce the phytotoxin, tabtoxin, their resistance to tabtoxin, and their host specificity. The possibility that the genes for these characteristics were plasmid-borne …
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Correlation between physical and genetic maps of plasmids
… study was initiated based on two families of plasmids with three different genes incorporated, namely the pGV 403 family which contains a Chloramphenicol resistance gene on one side and the pUC9 family which contains an Ampicillin and a Tetracycline resistance gene on the other side. Three …
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Transfer of plasmids by genetically-engineered Erwinia carotovora
… to transfer recombinant chromosomal DNA or plasmids to wildtype Ecc or <i>Pseudomonas fluorescens</i> was tested on filters, within soil microcosms, and <i>in planta</i>. Ecc was engineered by chromosomal insertion of a disarmed <i>endo</i>-pectate lyase gene marked with a 1.4kb DNA fragment …
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Properties of Three Plasmids Isolated From Plant Pathogenic Pseudomonads
Indigenous plasmids isolated from Pseudomonas tabaci ATCC 11528 (pJP1), P. tabaci BR2 (pBPW1), and P. angulata 45 (pJP30) were labeled with Tn3 using RSF1010::Tn3 as a vector and the host strains were subsequently cured of their Tn3-containing plasmid. Plasmid-containing and respective cured …
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Plasmids in Clostridium botulinum type A and Clostridium sporogenes
… botulinum type A and Clostridium sporogenes for plasmids was developed. Plasmid profiles of five C. botulinum type A strains and seven C. sporogenes strains were determined and a possible relationship of plasmids to toxin production was examined. The differentiation of these organisms by plasmid …
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Characterization of plasmids among the three species of Gluconobacter
… is known of their genetics, particularly, their plasmids. The overall purpose of this dissertation was to determine if Gluconobacter plasmids correlate with oxidative capability and/or antibiotic resistance. To achieve this goal, I first needed a way to screen strains of Gluconobacter for their …
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Detection and characterization of antibiotic resistance plasmids in Cheney biosolids
… were resistant to two or more drugs tested. Plasmids were separated from the resistant bacteria and 13.2% of them showed the presence of plasmids. These resistance plasmids were introduced into E. coli MM294 to screen for the presence of antibiotic resistance genes. Plasmids were isolated …
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Molecular Genetics in Rhodopseudomonas Sphaeroides: Transformation, Plasmids, and Gene Cloning
… were achieved using RSF1010 DNA, although other plasmids, such as pRK290 and RP4 were capable of transforming Tris-treated cells. A versatile cloning vector, pUI81, was constructed from RSF1010 and a pBR322 derivative, pSL25 and shown to be effective in transforming Tris-treated R. sphaeroides.
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Molecular and Genetic Relationships of Aromatic Metabolic Plasmids in Pseudomonads
… the similarities among the pseudomonad metabolic plasmids required for growth on the substrates naphthalene, NAH, salicylate, SAL, xylene and toluene, XYL and TOL. Naphthalene and xylene dissimilation pass through salicylate and toluate as intermediates to converge at catechol or its methylated …
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Relationship of plasmids in Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum
… objective of this study was to demonstrate that plasmids from clinical isolates are closely related to plasmids from environmental MAIS isolates. A 12.9 kb plasmid, pVT2, from a clinical <i>M. avium isolate</i>, MDl, was cloned and used a a DNA probe to examine the relationship of MAIS plasmids. …
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Plasmid stability of pUB110 and pUB110-derived plasmids in Bacillus sphaericus 2362
… cell division) of this plasmid and four plasmids derived from pUB110 was examined. The recombinant plasmids were developed in the laboratory of Dr. William Burke at Arizona State University. Stability was examined under various conditions of cell growth. Throughout spore germination, …
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Isolation and characterization of plasmids from human and environmental isolates of mycobacteria
… M. scrofulaceum (MAIS) complex were screened for plasmids in an effort to increase knowledge about the genetics and epidemiology of these pathogenic bacteria. Approximately 50% of the clinical MAIS isolates from New York, Maryland, Virginia, South Carolina, and Georgia contained one or more …
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A Novel Mechanism for Site-Directed Mutagenesis of Large Catabolic Plasmids Using Natural Transformation
… Pseudomonas putida is the natural host of TOL plasmids, ranging between 50 kbp and 300 kbp in size and encoding genes for the catabolism of toluene, meta-toluate, and xylene. These very large, single-copy plasmids are difficult to isolate, manipulate, or modify in vitro. In this study, the TOL …
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Genetic and Pathogenic Properties of Colicin v Plasmids in Invasive Strains of Escherichia Coli
Colicin V plasmids resident in strains of Escherichia coli are known to carry cistrons that augment the virulence of the host bacterium. Two assays were designed with which isogenic laboratory strains of E. coli K-12 with and without ColV plasmids were compared for their ability to adhere in vitro …
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Construction of green fluorescent protein plasmids for labeling of herbicide-degrading Delftia acidovorans MC1071
… to construct two green-fluorescent protein (GFP) plasmids to tag a specific strain of D. acidovorans (MC1071) for visual distinction from one or more competing microbes in a microfluidic pore structure that contains spatially varying concentrations of 2,4-DP, 2,4-D, and/or oxygen. Two GFP …
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