Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 3 of 3 for “"Plasmid cloning"”.
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Construction and phenotypic screening of mid-size insert marine microbial environmental genomic libraries
… inducible expression environmental genomic plasmid libraries for this purpose. A library with a mean insert size of 5.2 kilobases was constructed with environmental DNA isolated from surface ocean water collected at Hawaii Ocean Time-series station ALOHA in plasmid cloning vector pMCL200 …
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Mechanistic Insights into the Notch signalling pathway through Molecular Condensation
… I developed that allows for cost-effective plasmid cloning, I was able to develop both a light controlled Notch construct, OptoNotch, and a Live Notch target gene promoter reporter, called Hes1-Live-RNA, to allow for both the visualization and quantification of Notch activity within living …
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Characterization and cooperative dynamics of kinesin motor proteins in intracellular transport
… to address this problem. I designed a modular plasmid toolkit for controlled kinesin expression and purification, and I created a novel DNA origami scaffold that allows motors to be positioned with defined stoichiometry and geometry on a rigid cargo-like platform. Together, these advances …