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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 824 for “"Plasmid"”.
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Plasmid studies in Clostridium perfringens: Plasmid isolation; bacterial conjugation; interstrain mobilization of nonconjugative plasmids
Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:47:06Z Item is restricted indefinitely.
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Investigation of heterozygousity of yeast plasmid
… investigation of possible heterozygoisity of the plasmids found in the yeast species, specifically the 2-micron plasmid found in the yeast species. It explains the materials and methods used during this research, including the GATK software used to sequence the plasmid genome. An extensive table …
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Detection of Plasmid-Mediated AmpC b-Lactamases
Although plasmid-mediated AmpC b-lactamases were first reported in the late 1980's, most clinical laboratories and physicians remain unaware of their clinical importance. Current detection methods for organisms producing plasmid-mediated AmpC b-lactamases are time-consuming and …
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Studying plasmid relatedness and evolution through structural variation
… hundreds), and replicate independently, called plasmids. They are genetically extremely diverse, and in many cases are able to spread "horizontally" between unrelated cells. The ability to spread, while carrying genes coding for locally adaptive functions (e.g. antibiotic resistance, …
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Replication and stability of the linear plasmid pBSSB2
Plasmid pBSSB1 is a 27 kb linear DNA with proteins attached at the 5’ termini. It encodes the H: z66 flagellar antigen in Salmonella enterica serovar Typhi (S. Typhi) isolated from Indonesia. Together with the H: j or H: d flagellar antigen encoded by the host chromosome, pBSSB1 renders expression …
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Plasmid-influenced changes in Mycobacterium avium catalase activity
… avium strain, LR25, which carries 3 plasmids (18, 28, and 165 kb) and grows at 43°C was compared to its plasmid-free, avirulent segregant, strain LR163, to investigate the basis for the latter's inability to grow at 43°C. The failure of mid-log phase cultures of strain LR163 to grow …
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Investigation of a kalilo plasmid in Louisiana Neurospora tetrasperma
… is caused by the insertion of a linear plasmid, kalilo, into the host mitochondrial genome. Kalilo-related senescence has been studied extensively in Hawaiian field-isolated strains of Neurospora intermedia. This work, however, investigated the presence of a kalilo-like plasmid, named …
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Characterization of a 4.0 kilobase plasmid from Pasteurella multocida
A 4.0 Kb (2.64 Mdal) plasmid was isolated from a fowl cholera strain of Pasteurella multocida (the Larsen strain) by alkaline lysis and cesium chloride purification. The plasmid, designated pLAR-1, was characterized in terms of its size and restriction sites. The restriction patterns produced by …
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Analysis of the Nucleioprotein Complexes Essential for P1 Plasmid Partition
… division, and the same holds true for bacterial plasmids. Low-copy-number plasmids and bacterial chromosomes encode active partition systems to ensure their inheritance within a bacterial cell population. One of the well-studied models of partition is that of the P1 plasmid in E. coli. The …
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Actin-like filament A (AlfA) and AlfA-driven plasmid segregation
… A (AlfA) is a bacterial actin involved in plasmid segregation in Bacillus subtilis natto, and it is encoded by plasmid pLS32. In this dissertation, I investigate AlfA and the AlfA-driven segregation system, which is composed of the cytomotive AlfA filaments, the DNA-binding protein AlfB and …
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Cloning and sequence analysis of plasmid DNA from Helicobactor pylori
… gastric ulcers. Many bacterial species contain plasmid DNA which encodes virulence factors, including antibiotic resistance, adherence factors, degradative enzymes, and toxins. The majority of H. pylori strains contain plasmid DNA, although the size varies from strain to strain. No phenotype has …
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Spatiotemporal modelling in biology: from transcriptional regulation to plasmid positioning
… and spatial positioning of low copy number plasmids in Escherichia coli. Despite the diversity in biological subjects, my spatiotemporal modelling approach provides a common ground. FLC regulation involves an antisense-mediated chromatin silencing mechanism, where alternative polyadenylation …
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The Principles and Applications of Plasmid Transfer in Microbial Communities
<p>Conjugative plasmids can mediate the spread and maintenance of diverse traits and functions in microbial communities. This role depends on the plasmid’s ability to persist in a population. However, for a community consisting of multiple populations transferring multiple plasmids, the conditions …
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Purification of plasmid DNA for use in human gene therapy
… the focus of this research in manufacturing plasmid DNA for use In human gene therapy. First, the processing of E.coli bacterial cells to effect the separation of therapeutic plasmid DNA from cellular debris and adventitious material. Second, the affinity purification of the plasmid DNA in a …
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Structure and Function of the Yeast Chromosomal Replicator Ars1 (Plasmid)
… that allows the extra-chromosomal maintenance of plasmids in the yeast Saccharomyces cerevisiae. In order to define the functional domains of ARS1 and determine its role in plasmid maintenance, I have performed in vitro mutagenesis in the region containing ARS1 and measured their effects on Ars …
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Temperature adaptation and plasmid transformation in Listeria monocytogenes ATCC 7644
… and the second part was to develop a suitable plasmid transformation system using electroporation.
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Plasmid deoxyribonucleic acid of strains of the saccharolytic intestinal Bacteroides
… intestinal Bacteroides was examined for plasmid deoxyribonucleic acid (DNA). These strains were previously characterized for standard phenotypic properties (variety of carbohydrates fermented, metabolic end products, and complex carbohydrate hydrolysis). Additional phenotypic …
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The antitumor activity of tumor-targeted RNA replicase-based plasmid DNA
… we propose to deliver a RNA replicase-based plasmid DNA, hypothesizing that the dsRNA generated by the replicase-based plasmid in tumor cells will inhibit tumor growth. We evaluated the anti-tumor activity of a plasmid (pSIN-beta) that encodes the sindbis RNA replicase genes in mice with …
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