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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 71 for “"Plasma membranes"”.
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Characterization of Transfer RNA Associated with Plasma Membranes
RNA extracted from purified rat liver plasma membranes was found to contain transfer RNA. Amino acid acceptor activity was detected for arginine and lysine, a confirmation of previous reports. In addition, acceptor activity was also detected for tryptophan and proline. Isoaccepting tRNA species for …
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Studies on the Interaction of Adenylate Cyclase With Animal Cell Plasma Membranes
Made available in DSpace on 2014-12-14T04:27:41Z (GMT). No. of bitstreams: 1 7803983.pdf: 8352676 bytes, checksum: 3cd3a1a81163c7c8339507925fcfe2a3 (MD5) Previous issue date: 1977
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A Biochemical and Biophysical Comparison of Fetal and Adult Bovine Liver Plasma Membranes.
… were used to isolate high purity fractions of plasma membrane fragments from homogenates of adult and 5 month fetal bovine liver. Discontinuous sucrose density gradients were used during zonal centrifugation which yielded two fractions of plasma membrane from each source. In both the adult and …
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The Solubilization and Partial Purification of Adenylate Cyclase From Rat Liver Plasma Membranes
Made available in DSpace on 2014-12-14T04:27:43Z (GMT). No. of bitstreams: 1 7804138.pdf: 5451091 bytes, checksum: 7f0ae2a06a3c2cd130512e70d3f5ef4b (MD5) Previous issue date: 1977
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Binding of Insulin to Rat Epididymal Adipocytes and Purification of Their Plasma Membranes
Made available in DSpace on 2014-12-11T22:22:21Z (GMT). No. of bitstreams: 1 7317457.pdf: 7245817 bytes, checksum: 00fbfc363d00915673e1929baba1ebe1 (MD5) Previous issue date: 1972
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Hormone Induced Magnesium Transfer in Fat Cell Plasma Membranes: A Mechanism for Effecting Changes in Cellular Metabolism
… to measure magnesium transfer across the plasma membrane. This technique made use of the ligand, 8-hydroxyquinoline-5-sulfonic acid (8-OH-Q) which fluoresces in the presence of magnesium and was used to demonstrate epinephrine stimulation of magnesium uptake. The ligand was incorporated …
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Isolation and characterization of the (NAD(P)-independent)polyol dehydrogenase from the plasma-membranes of gluconobacter oxydans ATCC strain 621
… are catalyzed by constituitively synthesized plasma-membrane bound dehydrogenases. These bacteria would have to expend much energy to constituitively synthesize a separate substrate-specific dehydrogenase for each polyol substrate oxidized. Therefore, it is my hypothesis that Gluconobacter …
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Proteomic Analysis of Plasma Membrane Proteins from Drug Susceptible and Drug Resistant Breast Cancer Cell Lines
… the biotechnology and pharmaceutical industries. Plasma membranes are rich in drug targets and other proteins responsible for cell signaling, transport, signal transduction, and other cellular functions. Information obtained about these proteins, and the pathways they participate in, helps to …
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Quo vadis: quantitative evaluation of intracellular kinetics of siRNA delivered by poly-and-lipoplexes
… is of the same polarity as the net-anionic plasma membranes, making it exceedingly difficult to even approach the cells. As such, various materials have been developed to allow the delivery of the siRNA molecules to their primary sites of activity in cytosol. This, however, only solved the …
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Neurite inhibiting factors from the developing and mature spinal cord
… is currently the subject of cloning experiments. Plasma membranes purified from early embryonic chick and rat spinal cord were shown to provide a supportive substrate for the differentiation and outgrowth of neuroblastoma x glioma hybrid NG108-15 cells, in vitro. However, plasma membranes isolated …
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Kinetics of notch signaling during osteoclastogenesis
… and ligand located on the surface of adjacent plasma membranes. In osteoclasts, the Notch signal pathway has been shown to be inhibitory during early differentiation and critical for maturation and fusion during late differentiation. Cis interactions, receptor-ligand interactions that occur on …
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Cryo-electron Microscopy of Reconstituted Membrane Fusion Systems
… responsible for driving the fusion of biological membranes and are present across many different cell trafficiking systems. Synaptic SNARE proteins allow the release of neurotransmitters across synaptic clefts by promoting the fusion of vesicle membranes with neuron plasma membranes. In doing so, …
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Activity of Sodium Ion, Potassium Ion in the Epididymis of the Rat
… activity along the cytoplasmic side of the plasmalemma of the ductal epithelial cells. The most intense deposition of reaction products was found on the plasmalemma delimiting the lower lateral and basal regions of non-ciliated cells. The plasma membranes in the apical regions of the cells …
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The Ultrastructure of the Choroid Plexus in Vitamin A Deficient Rabbits
… blood cells, thrombocytes, and precipotated plasma proteins located in the subarachnoid space, 3) large cisterane between the plasma membranes of the "stretched" ependymal cells, 4) an apparent increase in the number of parallel and whorled forms of endoplasmic reticulum, 5) an apparent …
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Exploring the roles of key lipids in the influenza virus lifecycle
… cholesterol and sphingolipids form distinct plasma membrane domains, termed lipid rafts, that help recruit newly synthesized influenza components to virus assembly sites for progeny budding. However, the sphingolipid and cholesterol abundance at this bud zone remains a mystery due to the …
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The synthesis and in vitro evaluation of short interfering RNA containing triazole functionalized sphingolipid-based derivatives
… structural and functional components of plasma membranes of eukaryotic cells. Here, we design a library of siRNAs containing propyl-triazole modified sphingosine at various locations of the sense and antisense strand of the reporter gene firefly luciferase and evaluate its gene-silencing …
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Characterization of neural ion regulation dysfunction during insult and evaluation of micro-opioid receptor activation during simulated ischemia in the pre-botzinger complex
… to maintain ionic homeostasis across their plasma membranes. Even slight alterations in intracellular or extracellular can have a devastating effect on excitability and neural vitality. This thesis investigates several concepts related to ion flux. First, it investigates how ion flux affects …
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The effects of dietary lipids on the fatty acid composition of tissue and membrane fractions of spontaneous mammary adenocarcinomas and mammary glands of Strong strain A mice
The fatty acid composition of whole tissue and plasma membrane fractions of mammary adenocarcinomas and normal mammary glands excised from Strong A female mice fed a 15% safflower oil and 15% tallow diets were studied. Previous work has indicated that high fat diets in general and high unsaturated …
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