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Showing 1 to 9 of 9 for “"Plant cell culture"”.
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Exploiting alkaloid biosynthesis in Madagascar periwinkle to obtain natural product derivatives and new biocatalysts
Plant alkaloid biosynthesis produces many natural products with medicinal value. For example, vinblastine and vincristine from Catharanthus roseus monoterpene indole alkaloid biosynthesis, and camptothecin derivatives from Ophiorrhiza pumila quinoline alkaloid biosynthesis, are anticancer agents …
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Tomato Phytochemicals and Prostate Cancer Risk
… and phytofluene are not commercially available, plant cell culture methodologies utilizing the herbicide norflurazon and 14C-sucrose were developed to biosynthesize and radiolabel these carotenoids in tomato cell suspension cultures. DU 145 prostate tumor cells absorbed 14C-15- cis phytoene and …
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Studien zum Metabolismus von Pestiziden und Xenobiotika durch humane Cytochrom-P450-Monooxygenasen in transgenen Tabakzellkulturen
Detailed data on the metabolism of pesticides in plants is required for registration, therefore several in vitro systems have been developed to generate and identify a broad spectrum of possible metabolites. Therefore transgenic plant cell cultures with the human isoenzymes CYP1A1 and CYP1A2 were …
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The Breakdown of Plant Cell Biomass by Fungi
… for their ability to utilize a variety of plant cell substrates (methanol extracted), Catharanthus roseus, Daucus carota, re-autoclaved C. roseus, re-autoclaved D. carota) which preliminary studies had indicated contained the necessary nutrients for fungal growth. Incubated in a suitable …
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Characterization and application of large disposable shaking bioreactors
… to make it an ideal choice for cultivation of plant, animal and insect cell cultures for pilot-scale production. Cylindrical reactors of size 2L, 20L and 50L were thoroughly characterized in terms of important engineering parameters such as mixing, power consumption, heat transfer rate and …
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Development of microalgae as a biopharming platform
… project tested the feasibility of creating algal cell packs, a novel immobilised microalgae transient production platform for the expression of recombinant protein. First it had to be established whether the available plant expression vectors could be used for the transfer of genetic material into …
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Expression of anti-HIV peptides in tobacco cell culture systems
Submitted in fulfilment of the requirements for the Degree of Master of Technology: Biotechnology, Durban University of Technology, Durban, South Africa, 2009.
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Production and utilization of phytoene, phytofluene, and lycopene tracers for bioavailability and biodistribution studies
… of these tomato carotenoids, an in vitro plant cell culture system for production of isotopically labeled carotenoids was developed, and absorption and biodistribution studies were performed in a relevant animal model, the Mongolian gerbil (Meriones unguiculatus). To enhance 14C-carotenoid …
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Biotransformation studies on tobacco cembranoids using plant cell cultures
… studies on tobacco cembranoids using the plant cell culture lines coded as TRP4a and T-43-T which are derived from Tripterygium wilfordii, an important Chinese herbal plant, and Nicotiana sylvestris, respectively. The studies are divided into three parts: 1) Biotransformation of the diols …