Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 22 for “"Plant Transformation"”.
-
Advancing plant transformation and CRISPR/Cas editing: Strategies for recalcitrant maize
Plant genetic transformation is a pivotal tool for plant biology and crop improvement. While stable genetic transformation methods have been established, their applicability remains constrained to specific plant species and genotypes. The process is further characterized by labor-intensive …
-
The use of the broad-host-range plasmid, pTF-FC2, in Agrobacterium tumefaciens mediated plant transformation
… This transfer system has revolutionized plant molecular biology to the extent that the introduction of foreign genes into plant genomes is now a basic technique. Apart from the finding that a defined segment (T-DNA) of the A. tumefaciens Ti plasmid can be transferred to plant DNA, the …
-
Attempts toward the expression in transgenic Arabidopsis thaliana of Esi47, a salt stress-induced gene encoding a protein kinase from Lophopyrum elongatum
Plant transformation vectors for constitutive gene expression were prepared to study the role of Esi47, a gene that encodes a protein kinase, which normally is induced as part of the salt-stress response in Lophopyrum elongatum. Agrobacterium-mediated transformation of A. thaliana, by …
-
Expression and Localization of Foreign Proteins in Soybean and Tobacco Seed Tissue
Another objective of this research was to test a transformation procedure for soybean with an expression vector containing a casein gene, under the control of the seed-specific lectin promoter. Soybean is one of several crop plants which are still difficult to transform even in the hands of plant …
-
Adeno-associated virus rep78 expression in Arabidopsis thaliana
… for site-specific integration of transgenes in plants. This strategy would alleviate problems associated with current plant transformation methods that integrate transgenes randomly. The AAV-2 gene encoding the enzyme that catalyzes the insertion (rep78) was introduced into Arabidopsis thaliana …
-
Transgenic Plants, Current State, and Influence on the Environment
The techniques of plant transformation are discussed. They are bacterial-mediated, electroporation transformation, biolistic (particle bombardment), and microbeam laser. By using different strategies, foreign gene(s) from various organisms can be inserted into plant genome and expressed. …
-
Immunomodulation of Flavonoid Biosynthesis in Transgenic Arabidopsis thaliana
… the genes encoding the scFv's was cloned into a plant transformation vector, which was subsequently used to generate transgenic plants. One transgenic line with low expression of one of the scFv's appeared to have an altered flavonoid metabolism, as evidenced by a reduced capacity for anthocyanin …
-
Application of CRISPR/Cas9 to edit genes affecting seed morphology traits in wheat
… assay and choose the most efficient gRNAs for plant transformation. We successfully applied this pipeline to five wheat orthologs of the rice yield component genes that have been identified previously. We obtained edited plants for all these genes and validated the effect of the mutations in …
-
Promoter analysis in transgenic sugar beet
… of expression of foreign genes in the transgenic plants. A strategy has been defined for production of high sucrose yield ! high juice purity / low environmental impact varieties of sugar beet. This requires modification of the phytohormone levels in the outer cambial rings of the storage roots. …
-
Frameworks for reprogramming early diverging land plants
Plant form is a product of emergent processes of cell division, patterning and morphogenesis. These fundamental processes remain poorly characterised in plants. However, engineering approaches can provide new tools and frameworks for the study and manipulation of plant development. This …
-
The Consequences of Plant Anatomy on Viral Co-Infection
Plant viruses are infectious agents that exploit plants to survive, transmitted from host to host primarily by insect vectors. Within plant hosts, viral populations encounter both physical and physiological barriers that may influence their evolution. This work aims to identify opportunities during …
-
Metabolic engineering of the pterin branch of folate synthesis by over-expression of a GTP cyclohydrolase I in peanut
… (AtGCHI) was introduced into peanut by biolistic transformation. Plant transformation vectors were constructed using publicly available or licensable vector components to avoid intellectual property restrictions that hinder commercialization. Thirteen peanut cultivars were evaluated for …
-
Genetic engineering for pest resistance in potato (Solanum tuberosum L.)
… way for targeting genes of interest into the plant genome consists of Agrobacterium-mediated gene transfer. This thesis studied Agrobacterium-mediated transformation to transform two potato cultivars with an avidin gene anticipated to confer insecticidal activity against certain lepidopteran …
-
Expression of Human Protein C in Transgenic Tobacco
… g-carboxylation, and N-linked glycosylation. Plants have the eukaryotic protein modifying mechanisms required for many human proteins and may provide a safe, cost-effective system for producing hPC on a large-scale basis. Tobacco (Nicotiana tabacum L.) is particularly well suited for use as a …
-
Modifying N-linked glycosylation in tobacco to produce human-type post-translational modifications
Plant cells are a capable system for producing high value proteins for a variety of applications, and challenge popular host organisms such as mammalian cells, bacteria or yeasts. However, plants do not perform post translation protein modification in the same manner as mammalian cells, most …
-
Nanoelectroporation of single cell plants
… is hindered by the lack of production capacity. Plants such as Marchantia polymorpha, offer a promising, chassis for the scalable bioproduction of biologics. The prospects for distributed and low-cost production offer the potential to support rapid responses to emerging diseases and tackle global …
-
Transformation of Brassica napus cv. Westar with the beet western yellows virus coat protein gene
… BWYV CP was then cloned into plasmid pRT103, a plant expression vector which contains the cauliflower mosaic virus (CaMV) 35S promoter and polyadenylation signal. The CP cDNA was inserted in the plus-sense orientation (clone BW102D) and in the anti-sense orientation (clone BW137D) relative to …
-
Using a Mammalian Virus to Create Plants for Site-Specific Transgene Insertion
A novel strategy for site-specific DNA transformation of plants has been proposed and the first component of the system developed. The proposed method overcomes the limitations of current techniques by providing a specific integration site for the insertion of transgenes using features of the …
-
Improved regeneration and Agrobacterium-mediated transformation of wild strawberry (Fragaria vesca L.)
… to a short life cycle (12-16 weeks) and small plant size make F. vesca a good candidate for a model plant for genetic and molecular studies. The specific objective of this work was to develop an efficient high-throughput Agrobacterium-mediated transformation protocol to generate an insertional …
-
Studies on Transformation of Tomato(Solanum lycopersicum L.) and Arabidopsis thaliana using Chimerical constructs of varying Tospoviral Origin
… resistance (PDR) is a recent breakthrough where plant hosts can be made to be resistant to viral infections through transformation with conserved viral genes. Given the severity of Tospovirus diseases worldwide (particularly in tomato), PDR has the potential to garner large yield returns where …
Page 1 of 2