Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Planar Lipid Bilayer"”.
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Pilus biogenesis in uropathogenic Escherichia coli : An electrophysiology study on the PapC usher
… on the pore properties of the PapC usher. Using planar lipid bilayer technique we found that PapC forms an ion conducting channel that is mostly in a closed state. However, the usher channel is dynamic and transiently opens to various conductance levels. A structure function relationship study …
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GPCR Activation of the G Protein-Gated Inward-Rectifier Potassium Channel
… interact with purified GIRK1/4 channels in the planar lipid bilayer system. I conclude that protein co-localization is not the underlying mechanism to explain why GIRK channels are specifically activated by Gαi-coupled receptors. I then set out to determine the molecular basis behind signaling …
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Formazione di proteine canale: dal mitocondrio a SARS-CoV-2. Caratterizzazione funzionale e strutturale attraverso l'uso di tecniche respirometriche e di dinamica molecolare
… attraverso l’applicazione della tecnica Planar Lipid Bilayer. I risultati ottenuti hanno permesso di evidenziare una possibile distribuzione strutturale in grado di giustificare l’attività ionica del canale, ma soprattutto il meccanismo di regolazione di flusso ionico dovuto …
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Analisi elettrofisiologica delle proteine VDAC umane incorporate nei nanodischi e indagine sui residui di cisteina dell'isoforma 3 VDAC nella funzionalità mitocondriale e nel tamponamento dei ROS
I mitocondri sono organelli essenziali per le cellule eucariotiche poiché sostengono l'enorme richiesta energetica, necessaria per mantenere l'omeostasi cellulare. La comunicazione tra il citosol e i mitocondri è consentita dalle porine mitocondriali, note anche come Voltage-Dependent Anion …
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Structural characterization of bacterial membrane proteins via molecular dynamics simulations and electrophysiology
… success to date. I carried out a combination of Planar Lipid Bilayer electrophysiology experiments, Molecular Dynamics simulations, and protein homology modeling on the MtrE and the MtrC proteins. I characterized the MtrE conduit as being slightly cation selective, as opposed to other homologous …
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GPCR to Channel Communication in a Membrane Signaling Pathway
… regulate channels. How do different signaling lipids regulate the activity of ion channels? To address this question, the first part of the thesis presents an analysis of how phosphatidylinositol lipids regulate the gating of Kir2.2 channels. In this study, I employed the planar lipid bilayer …
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Isolation and Use of the Aeromonas hydrophila Secretin ExeD for Nanopore Analysis
… the ExeD pore would be inserted into a lipid bilayer in a patch clamp setup where observations could be made about the ability of ExeD to translocate small molecules and peptides. If ExeD could translocate larger peptides than α-hemolysin, nanopore analysis could be expanded to include …