Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Pioneer Factor"”.
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Molecular mechanisms of pioneer factor ELF2 interactions with the nucleosome
Pioneer factors are a subset of transcription factors (TFs) that can bind to nucleosomes and regulate gene expression at closed chromatin regions. Pioneer factors are important for stem cell pluripotency, cell reprogramming and differentiation. ETS family TFs are a large subfamily of TFs consisting …
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Sox2 co-occupies distal enhancer elements with cell-type-specific POU factors to specify cell identity in embryonic stem cells and neural precursor cells
… These data suggested that Sox2 might act as a pioneer factor for neural development throughout embryogenesis. While Sox2 is known to co-occupy target loci in ESCs with the POU factor Oct4, in NPCs Sox2 interacts with the central-nervous-system-expressed POU factors Brn1 and Brn2. By utilizing …
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The impact of ARID1A loss on ER+ breast cancer: mechanistic insights and therapeutic opportunities
… tumours exhibit a reduced reliance on the pioneer factor FOXA1, as evidenced by a marked collapse of the FOXA1 interactome. Conversely, these tumours transitioned to a BRD3-driven state, which was recruited to newly accessible genomic regions. This recruitment promoted transcription …
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GENOME-WIDE PROTEIN MOLECULAR ARCHITECTURE OF HUMAN PROMOTERS
Transcription factors (TFs) bind DNA cis-regulatory elements (CREs) in the context of chromatin to regulate the transcription machinery and gene expression. The precise positioning of the pre-initiation complex (PIC), TFs, cofactors, and nucleosomes within the human genome—and their interactions on …
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The role of transcription factors in somatic cell nuclear reprogramming by eggs and oocytes
… by overexpression of combinatorial Yamanaka factors (iPS, Takahashi et al., 2006). Although both approaches aim to derive cells with highest plasticity, the mechanisms and differences between these procedures are not yet clear. In my thesis, I used quantitative polymerase chain reaction …
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The Role of the Proneural Transcription Factor ASCL1 in Neuroblastoma Cell Division and Differentiation
… a core regulatory circuit (CRC) of transcription factors maintaining their highly proliferative, developmental state. In subtype MS neuroblastoma, tumours can spontaneously regress, in which case children are cured for life. The mechanisms behind spontaneous regression are poorly understood, but …
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Role of host chromatin remodelling in Mycobacterium leprae-induced Schwann cell fate change
… that Sox2, an early embryonic cell transcription factor, is reactivated in Schwann cells during this process. However, in the case of an infection by M. leprae, the bacteria hijacks this innate ability and manipulates the host cell machinery to keep the Schwann cells in this dedifferentiated state …
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Investigating upstream regulators of FOXA1 in breast cancer
FOXA1 is a lineage-defining transcription factor called pioneer factor. Due to its role in recruitment of nuclear receptors, such as estrogen receptor α (ERα) to enhancer regions, it is highly implicated in ERα positive breast cancer. Structural predictions of FOXA1 suggest a highly disordered …
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Investigating the transcription factor landscape of the squamous subtype of pancreatic ductal adenocarcinoma
… The Carroll Lab is interested in the role of pioneer transcription factors and their chromatin associated interaction partners in regulating transcriptional programs in different cancer types. This thesis has a particular focus on discovering the transcription factors involved in the …
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Mechanical Manipulation of Eukaryotic Chromatin by DNA-Binding Proteins
… how Sox2, which constitutes an important pioneer factor implicated in the maintenance of pluripotency, forms co-condensates with DNA and chromatin components. The described results present three conceptual advances to the field: 1) protein:DNA co-condensation can generate high forces, up …
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The Influence of DNA Methylation on Both H2A.Z Nucleosome Dynamics and the Zinc Finger and Homeoboxes Transcription Factor Family
… and Homeoboxes (ZHX) family of transcription factors. DNA methylation and H2A.Z preferentially occupy exclusive areas of the genome, biasing against their colocalization in the same genomic space. However, exactly how this mutual exclusion occurs has largely been left a mystery. We find that …
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Steroid receptor crosstalk in breast cancer cells.
… proteins, has also been shown to be an important factor in breast cancer development. FoxA1 has been shown to dictate ER binding in breast cancer cells and has been deemed responsible for the rapid reprogramming of ER signalling seen in breast cancers with poor outcomes and treatment resistance. …
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STRUCTURAL INSIGHTS INTO RNA POLYMERASE III PRE-INITIATION COMPLEX AND THE PIONEER ROLE OF OCT1
… and rely on the same core transcription factor, SNAPc. How a single complex enables mutually exclusive recruitment of two distinct RNA polymerases remains an unresolved question. To address this, we determined high-resolution cryo-electron microscopy (cryo-EM) structures of the human Pol …
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Computational modeling of gene expression from regulatory sequences
… sites for regulatory proteins (transcription factors, TF). The assembly of TFs bound on a CRM drives the desired expression level of the gene associated with the CRM. As the abundance of TFs vary across different cell types, the expression level of the gene, also termed as the “readout” of the …