Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Pilus biogenesis"”.
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Pilus biogenesis in uropathogenic Escherichia coli : An electrophysiology study on the PapC usher
… secretion system that is dedicated to the biogenesis and export of pili to the cell surface. It is comprised up of a periplasmic chaperone protein and an outer membrane usher protein that functions as the site of pilus assembly and simultaneous translocation. The usher exists as a dimer, …
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<em>Agrobacterium</em> Virb10 Contributions to Type Iv Substrate Secretion, T-Pilus Biogenesis, and Outer Membrane Pore Formation
… channel and a surface structure termed a T-pilus. This thesis is focused on identifying contributions of VirB10 to substrate transfer and T-pilus formation through a mutational analysis. VirB10 is a bitopic protein with several domains, including a: (i) cytoplasmic N-terminus, (ii) single …
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Characterization of The Role of The Pkm101-Encoded T4Ss Outer Membrane Core Complex In Substrate Transfer, Pilus Biogenesis, and Recipient Cell Contact
… to recipient plant or bacterial cells, (ii) pilus biogenesis and donor-specific bacteriophage sensitivity, and (iii) recipient cell contact. My results demonstrate that the C-terminal tails of VirB10 and TraF<sub>pKM101</sub> are essential for DNA transfer and pilus biogenesis, whereas the AP …
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Characterization of The Agrobacterium Tumefaciens Virb2 Pilin of The Virb/D4 Type Iv Secretion System
… channel spanning the cell envelope and a T-pilus extending from the cell surface. In A. tumefaciens, the VirB2 pilin subunit is required for assembly of the secretion channel and is the main subunit of the T-pilus. The focus of this thesis is to define key reactions associated with the T4SS …
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Characterization of an Interaction Between the Pseudopilin XcpT and the Putative ATPase XcpR in the Type II Secretion System of Pseudomonas Aeruginosa
… and pilin of both xcp secretion and Type IV pilus biogenesis, respectively). In this thesis, I have described the first genetic evidence of an interaction between two of the Xcp proteins, XcpT and XcpR. This was generated by isolating a temperature-sensitive mutation in the pseudopilin XcpT, …
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Computationally and Experimentally Exploring the Type IV Pilus Assembly ATPase for Antivirulence Drug Discovery
… disease development. The bacterial type IV pilus (T4P) is a prevalent virulence factor in many ABR pathogens, contributing to bacterial pathogenesis by facilitating cell motility, surface adhesion, and biofilm formation. Critically, the T4P facilitates early stages of disease, providing a …
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Insights Into Determinants That Contribute to Colonization, Virulence and Antibiotic Resistance In Enterococci
… elucidates a mechanism for the regulation of pilus expression. I showed that ATT is the initiation codon of <em>ebpA</em>, the first gene of an operon that codes for the <em>e</em>ndocarditis and <em>b</em>iofilm-associated <em>p</em>ili (Ebp), a recognized virulence factor in <em>E. faecalis. …