Global ETD Search
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Showing 1 to 8 of 8 for “"PilB"”.
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Understanding PilB, The Type IV Pilus (T4P) Assembly ATPase
… focuses on understanding the kinetics of PilB, the T4P assembly ATPase. PilB crystal structure exhibits an elongated hexamer with 2-fold symmetry indicating a symmetric rotary mechanism model. Except for its structure, the symmetric rotary mechanism of PilB has not been demonstrated …
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Structure-Activity Relationship Studies of Mitochondrial Uncouplers and PilB Inhibitors
… such as the type IV pilus construction enzyme PilB, is a strategy of treatment. PilB is a highly conserved hexameric ATPase responsible for pilus generation and is thus obligatorily involved in bacterial type IV pilus use. Previously, the type IV pilus has been shown to be critically important …
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The Type IV Pilus Assembly ATPase PilB as a Regulator of Biofilm Formation and an Antivirulence Target
… the regulation of the T4P assembly ATPase PilB. We examined its interactions with the secondary messenger cyclic-di-GMP (cdG). Specifically we investigated how cdG binding regulates PilB functions not only as the assembly ATPase, but also as an EPS signaling molecule in Myxococcus xanthus …
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Computationally and Experimentally Exploring the Type IV Pilus Assembly ATPase for Antivirulence Drug Discovery
… colonize a host. T4P assembly is driven by the PilB/PilF motor ATPase that localizes to the cytoplasmic face of the inner membrane to drive pilus biogenesis by ATP hydrolysis. The thesis work here explores computational and experimental methods for the discovery of antivirulence therapeutics …
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Recruitment and Mechanism of the Bacterial Type IV Pilus Motors
… protein fibre from the bacterial inner membrane. PilB and PilT are the cytoplasmic ATPases that power pilus extension and retraction, respectively. PilC is an inner membrane protein that bridges the cytoplasmic ATPases to the pilus. PilM is a cytoplasmic protein that is predicted to recruit PilB …
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Molecular Investigations of Protein Assemblies Involved in Prokaryotic Virulence
… is powered by a dedicated cytoplasmic ATPase, PilB. The structural study of PilB from a related system (chapter 2) resulted in the formulation of the first model describing the mechanism of force generation resulting from ATP hydrolysis, which explains how T4P are assembled. Chapter 3 focuses …
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Use of an Inducible Promoter to Characterize Type IV Pili Homologues in Clostridium perfringens
… them in cells growing on surfaces. We saw that PilB1 and PilB2 co-localized nearly all of the time, while a portion of PilT was independent of the PilB proteins. PilT appeared necessary for the localization of PilB, and it localized independently of TFP proteins in <i>Bacillus subtilis</i>. …
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Localization of Type IV Pilin Polymerization Proteins in Clostridium perfringens
… We studied the localization of TFP ATPases, PilB1, PilB2 and PilT in Bacillus subtilis to compare the localization in an organism other than C. perfringens and which lacks any known genes similar to TFP. Unlike the case in C. perfringens, PilB1 in B. subtilis localized to the poles in the …