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Showing 1 to 3 of 3 for “"Pik1"”.
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The Development of a Time-Resolved, Vesicle-Based, Fluorescence Assay for the Activity of the Lipid Kinase Pik1
Pik1 is a yeast phosphatidylinositol-4 kinase that is critical for vesicular traffic to the plasma membrane and endosomes from the trans-Golgi (1, 2). Pik1 is a soluble enzyme, and the small myristoylated, Ca2+ binding, EF hand protein, Frequenin (Frq1) facilitates its membrane localization (3). It …
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Novel regulators of trafficking in the yeast Golgi-endosomal system
… (PI(4)P) generated by the PI(4)-kinase Pik1p plays an essential role in maintenance of Golgi secretory function and morphology. Up to now relatively few targets of Pik1/PI(4)P signaling at the Golgi have been identified and it thus remains elusive how Pik1p mediates its essential …
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THE OPTIMIZATION OF A TIME-RESOLVED, VESICLE-BASED FLUORESCENCE ASSAY FOR THE ACTIVITY OF THE LIPID KINASE PI4K-IIIBETA AND THE EFFECT OF LIPID TRANSFER PROTEINS ON ENZYME ACTIVITY
… PI to be phosphorylated by the PI4-kinase Pik1 in a heterotypic ligand exchange fashion. However, the precise mechanism by which this interaction occurs has yet to be elucidated. To explore how and if PITPs and PI4K-IIIβ work as hypothesized, we expressed and purified recombinant human …