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Showing 1 to 4 of 4 for “"Pig oocytes"”.
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Factors influencing in vitro maturation of pig oocytes
… of estradiol-17$\beta$ on in vitro maturation of pig oocytes. Cumulus oocyte complexes (COC) were obtained by aspiration of 2-5 mm follicles. The oocytes were cultured for 42 hours at 38.5$\sp\circ$C in mTCM-199 supplemented with LH, FSH, follicular fluid, fetal calf serum and estrous gilt serum, …
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Advances in the fundamental cryobiology of mammalian oocytes
Having effective means to cryopreserve mammalian oocytes could increase the efficiency of managing populations of laboratory animals, increase the effectiveness of breeding programs for livestock, and improve the means by which assisted reproductive therapy is applied to human patients. …
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STUDIES IN OOCYTES FROM THREE MAMMALIAN SPECIES DEMONSTRATE THAT MEIOTIC KINETOCHORES ARE COMPOSED OF PREVIOUSLY UNIDENTIFIED SUBDOMAINS AND REVEAL TWO NOVEL MECHANISMS BEHIND THE MATERNAL-AGE EFFECT IN HUMANS
… of meiosis I. My analysis revealed that human oocytes challenge the paradigm that sister kinetochores are fully fused. Instead, I demonstrated that sister kinetochores disjoin as women get older, which promoted erroneous kinetochore-microtubule attachments. This in turn allowed chromosomes to …
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The Effect of Growth Hormone on Pig Embryo Development in Vitro and an Evaluation of Sperm-Mediated Gene Transfer in the Pig
… (IVM) affected subsequent embryo development. Pig cumulus-oocyte complexes (COC) (n=987) were aspirated from slaughterhouse derived ovaries and cultured in BSA-free NCSU 23 medium containing porcine follicular fluid (10% v/v), cysteine (0.1 mg/ml) and hormonal supplements (eCG and hCG, 10 IU/ml …