Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 46 for “"Picomolar"”.
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Sub-Picomolar Detection of SARS-CoV-2 RBD via Computationally-Optimized Peptide Beacons
… S-RBD with limits of detection (LoD) in the sub-picomolar range. We envision that these beacons can be easily integrated with on-chip optical sensors to construct a point-of-care diagnostic platform for SARS-CoV-2.
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Microfluidic diffusional sizing at the single molecule level
… capabilities at performing diffusional sizing at picomolar sensitivity. Microfluidic diffusional sizing (MDS) is a robust, in-solution method to measure hydrody- namic radii (Rhyd) of particles. The range of MDS is very much suited to measuring the size of individual proteins and higher-order …
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Novel tools to identify estrogen regulated genes important for breast and ovarian cancer cell proliferation
… cancer in a post-menopausal woman is in the picomolar range. However, the known binding affinity of E2 for ER lies between 0.5 and 2 nM. It was not known whether E2 concentrations in the picomolar range can exert biological effects on cell proliferation and gene expression. Moreover, the …
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Interleukin-2 Engineering for improved therapeutic effectiveness
… (K[d] [approximately] 10 nM). IL-2 mutants with picomolar affinity for IL-2Rα stimulate T cell growth responses quantitatively equivalent to those mediated by IL-15. Our results suggest that the contrasting effects of IL-2 and IL-15 on T cells in vivo are largely due to the 1,000-fold different …
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Interactions of cadmium, zinc, and phosphorus in marine Synechococcus : field uptake, physiological and proteomic studies
… Synechococcus WH5701 cells exposed to low picomolar quantities of free Cd under Zn deficiency show similar growth rates to no added Cd treatments during exponential growth phase, but show differences in relative abundances of many proteins involved in carbon and sulfur metabolism suggesting …
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Interactions of cadmium, zinc, and phosphorus in marine Synechococcus : field uptake, physiological and proteomic studies
… Synechococcus WH5701 cells exposed to low picomolar quantities of free Cd under Zn deficiency show similar growth rates to no added Cd treatments during exponential growth phase, but show differences in relative abundances of many proteins involved in carbon and sulfur metabolism suggesting …
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Amyloid-beta signaling in physiology and pathology: from astrocytes to SUMO
… demonstrated that physiologically-relevant (low picomolar) amounts of Abeta can enhance synaptic plasticity and memory. Astrocytes, as crucial glial support cells with roles in modulating synaptic transmission, are likely cellular candidates for participating in this type of physiological Abeta …
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Natural and synthetic estrogens in wastewater treatment plant effluent and the coastal ocean
… are detrimental to aquatic organisms at picomolar concentrations, many studies have measured so-called “free” estrogen concentrations in wastewater effluents, rivers, and lakes. Yet, to our knowledge, no studies have characterized the broader range of estrogens that includes free, …
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Natural and synthetic estrogens in wastewater treatment plant effuent and the coastal ocean
… are detrimental to aquatic organisms at picomolar concentrations, many studies have measured so-called "free" estrogen concentrations in wastewater effluents, rivers, and lakes. Yet, to our knowledge, no studies have characterized the broader range of estrogens that includes free, …
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Engineering Soluble, High Affinity Receptor Antagonists for Bacterial Exotoxins
… describes the engineering of murine Vbeta8.2 for picomolar affinity to staphylococcal enterotoxin B (SEB). As the known contact regions had already been heavily mutagenized, additional engineering of the Vbeta was performed through extension of the CDR1 loop. Soluble forms of the high-affinity …
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EFFECTS OF COMPLEXATION WITH THE SIDEROPHORE DESFERRIOXAMINE B ON TRANSITION METAL REMOVAL FROM SEAWATER
… and found in open ocean surface waters at low-picomolar concentrations. Stability constants for DFOB complexes with divalent metals are similar to conditional constants that have been measured in seawater for highly metal-specific, but unidentified organic ligands. Titration data indicate that …
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Biomarkers of Lipid Oxidation in the Oral Cavity
… these analytes are found at extremely low (picomolar to nanomolar) concentrations, preconcentration of samples prior to gas chromatography-mass spectrometry analysis is crucial. This study utilizes both solid phase microextraction (SPME) and micromachined silicon micropreconcentrators to …
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INSTRUMENTAL ADVANCES IN CAPILLARY ELECTROPHORESIS MASS SPECTROMETRY FOR TRACE-SENSITIVE PROTEOMICS
… advances including detection of peptides at sub-picomolar levels (30 zmol), robust analysis of 100 nL proteome samples, and levitational sample enrichment by 4.4 folds. Collectively, this dissertation contributes to the leaping development of CE-MS for proteomics.Chapter 1 overviews the …
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The Development and Application of Ultra-sensitive Protein Aggregate Detection Techniques
… assays exhibit high sensitivity in the lower picomolar range with satisfactory precision. The aggregate quantification assay developed was then used in biomarker discovery for AD diagnosis. The multifaceted approach, combining individual assay measurements and ratios, exhibited a more reliable …
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Raman Spectroscopy for Monitoring of Microcystins in Water
… (SERS) it is possible to achieve femto or picomolar detection limits for a variety of target species. Drop coating deposition Raman (DCDR) was successfully implemented for quantitation of 2-100 ng of MC-LR deposited in 2 ?L of aqueous sample, even without the use of a specifically designed …
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Identification of native co-factors of MshB and MCA from Mycobacterium species
… MshB and MCA affinity for Zn(II) to be in the picomolar range and Ms MshB affinity for Fe(II) in nanomolar range. These results indicate that MshB and MCA can be found bound with either iron or zinc and this is independent to their affinities for these metal ions.
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Natural and Synthetic Ligand-Binding Induce Different Pilin Assemblies in vitro and Control P. aeruginosa Bioactivities in vivo, and Development of Bacterial-Motility Enabled Binding Assays
… Pilin monomers bind to the rhamnolipids at picomolar ranges and induce pilin proteins to form linear nano-assemblies. About one pico-molar of the ligands causes the transition of fluorescence signal to plateau for 100 nM of pilin monomers. This 10-5 equivalence effect is likely due to tight …
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Synthesis of Bulky and Polar Galactonoamidines for the Inhibition of the Human α-Galactosidase
… inhibition constants in the low nanomolar to picomolar range. The work described here summarizes an effort towards the synthesis of polar and bulky galactonoamidines for the inhibition of the human α-galactosidase. Following a protocol previously established in the group, the precursor …
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Design, Synthesis, and Screening of Novel Phosphatidylinositol 3-Kinase (PI3K) Inhibitors Activated by Prostate Specific Antigen (PSA): Prodrug Treatment for Androgen-Independent Prostate Cancer
… suggest PI3K enzyme inhibition in the nanomolar/picomolar range. Type I PI3K inhibitors contain: (1) a pyrimidine or triazine core, (2) a secondary amine - usually a morpholine ring, and (3) an aromatic/heteroaromatic group capable of hydrogen bonding. Type II PI3K inhibitors contain these same …
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Luminescence-Based MicroRNA Detection Methods
… (nanomolar) level down to the femtomole (picomolar) level. These methods are rapid, sensitive, simple, and quantitative, can be employed in complex matrices, and do not require expensive instruments. All methods are hybridization-based and do not require amplification steps.
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