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Showing 1 to 20 of 77 for “"Pichia pastoris"”.
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Calcium homeostasis in Pichia pastoris
<p>Pichia pastoris is a methylotrophic yeast that has been used widely in biological and industrial researches. P. pastoris is able to produce from milligram to gram quantities of protein. In this study, we examined the effects of calcium and magnesium on growth, heterologous protein expression, …
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Cloning and characterization of MET2 in Pichia pastoris
<p>The methylotrophic yeast, Pichia pastoris, has been used as a protein expression system to express over 500 heterologous proteins. P. pastoris provides many advantages over other organisms that have been utilized for this purpose. In this project, we developed a new host/selectable marker and …
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Cloning and expression of xylanase variants in Pichia pastoris
Submitted in fulfilment for the requirement of a Degree of Master in Biotechnology, Durban University of Technology, Durban, South Africa, 2017.
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Cloning and characterization of the Pichia Pastoris PMR1 gene
<p>Pichia pastoris, a popular protein expression system, is limited in its ability to secrete heterologous proteins. The PMR1 gene, the disruption of which is known to improve the secretion of prochymosin, human prourokinase, and human tissue plasminogen activator in Saccharomyces cerevisiae, was …
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Characterization of the Pichia pastoris alcohol oxidase I promoter
<p>The methylotrophic yeast, Pichia past oris, is one of the most respected and widely used systems today. The ability of this yeast to produce large masses of protein and metabolize methanol as a sole source of carbon and energy is attributed to the highly induceable Alcohol Oxidase I promoter …
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Establishment of a novel Pichia Pastoris host production platform
… densities in low-cost media. The yeast strain, Pichia pastoris, combines these advantages with the ability to secrete glycosylated products at equivalent product quality levels as CHO-based processes.
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Clonal selection and characterization of epigenetic variation in Pichia pastoris
… of the heterologous protein production host Pichia pastoris, which resulted in overall improvement of the secretion rate of the mutated population. More than 4000 single-cells were simultaneously screened for high secretion of a human Fc fragment using microengraving and the top-producing …
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Microbubble fermentation of recombinant Pichia pastoris for human serum albumin production
The high cell density fermentation of recombinant Pichia pastoris for human serum albumin (HSA) production is a high oxygen demand process. The oxygen demand is usually met by increased agitation rate and use of oxygen-enriched air. Microbubble fermentation however can supply adequate oxygen to the …
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Characterization of components that increase secretion of recombinant proteins in pichia pastoris
<p>Pichia pastoris is a methylotrophic yeast that is commonly used for its ability to express and secrete heterologous proteins. However, some proteins are not readily secreted in P pastoris and so adjustments in the secretion pathway must be made in order to achieve secretion. The Lin-Cereghino …
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Structural characterization of the MATα prepro-peptide secretion leader in Pichia pastoris
<p>The methylotrophic yeast, Pichia pastoris, is the most successful and favored microbial eukaryotic expression system for the production of recombinant proteins for biopharmaceutical or industrial purposes. P. pastoris has the ability to produce foreign proteins at high levels extracellularly, …
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A central composite design to investigate antibody fragment production by Pichia pastoris
This study aims to investigate the relationships between growth parameters (agitation, glycerol concentration, salt concentration) and responses (biomass, growth rate, protein expression), by a 3-factor-3-level central composite factorial design. This experimental design involved running shake …
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Recombinant Expression In Pichia pastoris System Of The Potassium Channel Inhibitor Peptide, Ts6.
… kDa. In the scope of this research, we used X-33 Pichia pastoris yeast which produced Ts6 extracellularly in substantial amount. The plasmid vector pPICZαA-Ts6 was constructed, where the N-terminal was his-tagged and an enterokinase cleavage site was inserted. The X-33 Pichia clones were cultured …
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Characterizing potential secretion components that increase secretion of recombinant proteins in Pichia Pastoris
<p>The methylotropic yeast Pichia pastoris has been used for many applications, particularly for its ability to produce and readily secrete heterologous proteins. Nonetheless, there are obstacles in making this useful yeast into a more efficient secretion system that readily secretes problem …
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Characterizing phenotypes of Pichia pastoris mutants that show enhanced secretion of recombinant proteins
… of the Pacific created mutant strains of Pichia pastoris using the restriction enzyme mediated integration method. The mutants exhibited an unusual ability to supersecrete beta-galactosidase, due to the effects of a randomly disrupted gene by pREMI-Z. To learn more about the novel effects …
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Biosynthesis of Cucurbita maxima trypsin inhibitor I in the methylophic yeast Pichia pastoris
… these shortcomings, the methylotrophic yeast Pichia pastoris was investigated as an alternative means of expression, although at the inception of this work, no disulphide-rich proteins of this size had been expressed in P. pastoris. It was a challenge to investigate the feasibility of …
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Biosynthesis of Cucurbita maxima trypsin inhibitor I in the methylotropic yeast Pichia pastoris
… these shortcomings, the methylotrophic yeast Pichia pastoris was investigated as an alternative means of expression, although at the inception of this work, no disulphide-rich proteins of this size had been expressed in P. pastoris. It was a challenge to investigate the feasibility of …
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Identification and characterization of components that overcome secretion limitations of the yeast Pichia pastoris
<p>The methylotrophic yeast. Pichia pastoris, is a powerful, adaptable, and inexpensive recombinant expression system commonly used to secrete heterologous protein. Although P. pastoris is a popular host organism, secretion inefficiency continues to be a major hurdle in its ability to produce high …
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Expression and functional characterization of the recombinant spider protein GW2 in yeast Pichia pastoris
<p>The chairperson of the candidate's dissertation committee is responsible for securing the signature of each committee member and the grade, which she/he wishes to assign, to be entered in the appropriate spaces below. Most dissertations are graded on a pass (P) or no credit (NC) basis. The …
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