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Showing 1 to 16 of 16 for “"Phytohemagglutinin (PHA)"”.
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Cell-mediated immunity in Marek's disease virus-infected chickens genetically selected for high and low levels of plasma corticosterone
… serum albumin (DNP-BSA) conjugate. Phytohemagglutinin (PHA-P) was used to assess non-specific lymphocyte transformation. MDV-infected HPC and LPC chicken leucocytes were also compared for their ability to lyse ⁵¹Cr-labeled fowl erythrocytes upon stimulation with PHA-P. Leucocytes …
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Glucocorticosteroid modification of lymphocyte blastogenesis in fibrosarcoma-bearing mice
… mice showed a 2-fold suppression of phytohemagglutinin (PHA) responsiveness with the addition of 0.1 µg HCS. However, T cells from mice with 3-4 week tumors did not demonstrate as great a suppression, if any. Kinetic studies during tumor growth demonstrated a decrease in HCS …
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Proteinaceous inhibitors of α-amylase and α-glucosidase from common bean (Phaseolus vulgaris L.): biochemical characterization and phylogenetic analysis of Sardinian cultivars
The common bean Phaseolus vulgaris is a good source of protein fiber and bioactive compounds. Among these proteinaceous α-amylase inhibitors (α-AIs) and α-glucosidase inhibitor could play an important role in weight loss and control of glycemic index above all in overweight and diabetic subjects. …
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Phenotypic Analysis and Localization of Leukocytes at the Site of Intradermal Injection of Phytohemagglutinin in Chickens
<p>Phytohemagglutinin (PHA) is commonly used to gain insight into an individual’s cellular immuno-competence. PHA is typically injected intradermally (i.d.) into the skin (e.g. wing web) and the tissue swelling response is then monitored, whereby the extent of the swelling positively relates to the …
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B and T Lymphocytes in Human Pregnancy
… when exposed to the following mitogens: phytohemagglutinin (PHA), pokeweed mitogen (PWM) and concanavalin A (Con-A). Blast transformation was measured by increased incorporation of <sup>3</sup>H-thymidine, Maternal lymphocytes showed a peak reactivity when exposed to PHA and PWM but not …
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The Mode of Action of Immunoregulatory Alpha Globulin (IRA) on Murine Lymphocytes: Relation of the Immunosuppressive Activity of Various Pathologic Conditions
… there are immunosuppressive components in the alpha globulin fractions of human and murine sera (1, 2, 3, 4, 5, 6, 7, 8, 9). These investigations shed new insights on the mechanisms controlling immune responses in normal individuals, and may elucidate the specific mechanisms involved in producing …
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Effects of dietary tyrosine and tryptophan supplementation on immunity and brain neurotransmitter levels after SRBC injections in chickens
… the effects of dietary tyrosine or tryptophan supplementation on immunity and brain neurotransmitter levels after antigen challenge. Chickens were given 0.1%, 0.5% or 1% supplemental dietary L-tyrosine or L-tryptophan prior to the injection of sheep red blood cells (SRBC). The 0.1% …
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Potential Downstream Immunological Effects of Evolved Disease Tolerance in House Finches
… 20+ years of MG endemism. When challenged with phytohemagglutinin (PHA), populations differed significantly in the strength of wing web swelling, with populations with longer MG exposure (and thus the highest MG tolerance) on average exhibiting the weakest swelling response when mass differences …
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The Effect of Dietary Fat Level on Tumor Development and Immunity in BALB/C Mice Fed Casein of Wheat Gluten as Protein and Challenged with Herpes Type 2-transformed Cells
… lymphocyte transformation test (LTT) using phytohemagglutinin (PHA), concanavalin A (ConA) or lipopolysaccharide (LPS) as mitogens.</p> <p>The type of protein present in the diets of the mice (casein or wheat gluten) appeared to be the most important factor influencing tumor development. …
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In Vitro Evaluation of Cancer Patient Immune Responses Following Infusion of Radiolabeled Murine Monoclonal Antibody
… infusion. However, this was not observed when phytohemagglutinin (PHA) was used as the stimulating agent. In contrast, the lymphoblastogenic response of patient’s cells to PHA (but not to MAb) was significantly lower when compared to cells from healthy subjects. A significant positive …
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Toxic effects of dietary methylmercury on immune system structure, function, and development in the American kestrel (Falco sparverius)
… included cell-mediated immunity (CMI) using the phytohemagglutinin (PHA) skin-swelling assay and antibody-mediated immune function using the sheep red blood cell (SRBC) hemagglutination assay. Hematology profiles were evaluated over time in adult males and nestlings to monitor immunological and …
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Caracterização da microbiota intestinal, perfil inflamatório e integridade da barreira gastrointestinal de crianças diagnosticadas com transtorno do espectro autista
… resistance gene analysis. Relative abundance, alpha diversity, and beta diversity analyses were performed to characterize the microbiota and resistance genes, along with differential relative abundance analysis. Zonulin, Claudin, and Occludin concentrations were analyzed from fecal samples. The …
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The application of Near Infrared Transmittance (NIT) individual kernel sorting technology to improve grain quality from spring and durum wheat infected with Fusarium and the effects on broiler chicken performance and immune response.
… response to injection of the T cell mitogen phytohemagglutinin (PHA); 2) humoral response to immunization with bovine serum albumin (BSA) antigen; 3) relative weights of liver, spleen and bursa of Fabricius; 4) heterophil to lymphocyte (H:L) ratio; and 5) histopathology of primary and …
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Effect of Time and Diet on the Immune Status of Mice Challenged with Herpes-transformed Cells
… lymphocyte transformation test (LTT) using both phytohemagglutinin (PHA) and lipopolysaccharide (LPS) as mitogens. Stimulation indices (S.I.) were calculated using the means counts per minute (cpm) obtained from triplicate samples. Spleen cell differential counts (S.C.D.C.) were also performed on …
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Cellular immune ontogeny and birth transcriptome in HIV-exposed uninfected infants
… (TT), Bordetella pertussis (BP) antigens and Phytohemagglutinin (PHA). Multi-parameter flow cytometry was used to measure batched whole blood samples for (i) markers of T cell regulation and activation directly ex vivo, markers of T cell gut homing and proliferation—a proxy for HIV …
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ΓΟΝΙΔΙΑΚΗ ΕΚΦΡΑΣΗ ΔΙΕΓΕΡΜΕΝΩΝ ΑΝΘΡΩΠΙΝΩΝ Τ-ΛΕΜΦΟΚΥΤΤΑΡΩΝ: ΡΥΘΜΙΣΗ ΤΗΣ ΕΚΦΡΑΣΗΣ ΤΟΥ ΓΟΝΙΔΙΟΥ 2 ΚΑΤΑ ΤΗ ΔΙΑΡΚΕΙΑ ΤΟΥ ΚΥΤΤΑΡΙΚΟΥ ΚΥΚΛΟΥ
… AND REGULATION OF THE CD2 GLUE EXPRESSION IN PHA STIMULATED HUMAN PERIPHERAL BLOOD T-LYMPHOCYTES.THE ACCURATE POSITION OF STIMULATED LYMPHOCYTES IN THE CELL CYCLE WAS DETERMINED BY USING THE "PREMATURE CHROMOSOME CONDENSATION" METHOD (PCC), WHICH OFFERS THE POSSIBILITY OF STUDYING THE …