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Showing 1 to 20 of 25 for “"Phytohemagglutinin"”.
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Phenotypic Analysis and Localization of Leukocytes at the Site of Intradermal Injection of Phytohemagglutinin in Chickens
<p>Phytohemagglutinin (PHA) is commonly used to gain insight into an individual’s cellular immuno-competence. PHA is typically injected intradermally (i.d.) into the skin (e.g. wing web) and the tissue swelling response is then monitored, whereby the extent of the swelling positively relates to the …
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Immune Responses and Hematologic Alterations in Splenectomized Pigs Experimentally Infected With Eperythrozoon Suis: I. A Possible Animal Model for Cold Agglutinin Disease in Man
… in vitro of peripheral blood lymphocytes with phytohemagglutinin mitogen, pokeweed mitogen, lipopolysaccharide (E. coli) mitogen, porcine erythrocyte stromal antigen, and Eperythrozoon suis particulate antigen. Humoral immune responses were evaluated by serum indirect hemagglutination titers …
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Effects of lead ingestion on the immune function in quail
… immune response to an intradermal injection of phytohemagglutinin. Corticosterone suppressed cell-mediated immunity more than lead. The primary total antibody response to chukar (Alectoris graeca chukar) erythrocytes was significantly suppressed in lead-dosed quail fed com. Lead also reduced the …
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Effects of the Removal of Adherent and Phagocytic Cells on the Spleen Cell Lymphoproliferative Response of Tumor Bearing Mice
… using the lymphocyte transformation test with phytohemagglutinin as the mitogen. This lymphoproliferative response was quantitated using the Stimulation Index (SI). Spleen cells from mice injected with #51 cells, a chemically-transformed colon cell line, produced a SI value equal to that of …
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Cell-mediated immunity in Marek's disease virus-infected chickens genetically selected for high and low levels of plasma corticosterone
… serum albumin (DNP-BSA) conjugate. Phytohemagglutinin (PHA-P) was used to assess non-specific lymphocyte transformation. MDV-infected HPC and LPC chicken leucocytes were also compared for their ability to lyse ⁵¹Cr-labeled fowl erythrocytes upon stimulation with PHA-P. Leucocytes …
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Glucocorticosteroid modification of lymphocyte blastogenesis in fibrosarcoma-bearing mice
… mice showed a 2-fold suppression of phytohemagglutinin (PHA) responsiveness with the addition of 0.1 µg HCS. However, T cells from mice with 3-4 week tumors did not demonstrate as great a suppression, if any. Kinetic studies during tumor growth demonstrated a decrease in HCS …
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Proteinaceous inhibitors of α-amylase and α-glucosidase from common bean (Phaseolus vulgaris L.): biochemical characterization and phylogenetic analysis of Sardinian cultivars
… inhibitors, antinutritional factor phytohemagglutinin (PHA), and about the expression of α-AI gene and their phylogenetic relationship. All the cultivars presented α-glucosidase inhibitor activity, while α-AI was missing in two of them. Only Nieddone cultivar (ACC177) had no …
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B and T Lymphocytes in Human Pregnancy
… when exposed to the following mitogens: phytohemagglutinin (PHA), pokeweed mitogen (PWM) and concanavalin A (Con-A). Blast transformation was measured by increased incorporation of <sup>3</sup>H-thymidine, Maternal lymphocytes showed a peak reactivity when exposed to PHA and PWM but not …
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The Mode of Action of Immunoregulatory Alpha Globulin (IRA) on Murine Lymphocytes: Relation of the Immunosuppressive Activity of Various Pathologic Conditions
… lymphocytes following stimulation with either phytohemagglutinin (PHA) or specific antigens. | This present study was undertaken to evaluate the effects of human alpha globulins (Cohn Fraction IV) and/or sera from donors with a variety of pathological conditions on the response of cultured …
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Effects of dietary tyrosine and tryptophan supplementation on immunity and brain neurotransmitter levels after SRBC injections in chickens
… 9 and broiler secondary IgM titers at Day 9. The phytohemagglutinin (PHA) wattle response in Leghorns and broilers and resistance of Leghorns to E. coli challenge were not affected with the 0.1% supplemental level. With higher levels, 0.5% tyrosine supplementation increased Leghorn primary IgM …
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Immunological and hematological biomarkers for contaminants in fish-eating birds of the Great Lakes
… In Caspian tern and herring gull chicks, the phytohemagglutinin skin test for T cell function showed a strong negative exposure-response relationship with organochlorines. There was no discernible association between contaminants and suppression of antibody-mediated immunity as measured by the …
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Potential Downstream Immunological Effects of Evolved Disease Tolerance in House Finches
… 20+ years of MG endemism. When challenged with phytohemagglutinin (PHA), populations differed significantly in the strength of wing web swelling, with populations with longer MG exposure (and thus the highest MG tolerance) on average exhibiting the weakest swelling response when mass differences …
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Lymphocyte Activation, T-Cell Cytotoxicity and Their Relationship to Dietary Fat Enhanced Murine Mammary Tumorigenesis
… to the T-cell mitogens, concanavalin A and phytohemagglutinin was 20%-25% lower in mice fed 20% SBO compared to mice fed 5% SBO after twenty weeks of feeding. There was no effect of SBO concentration on the splenocyte response to the B-cell mitogen, lipopolysaccharide or to the B- and T-cell …
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The Effect of Dietary Fat Level on Tumor Development and Immunity in BALB/C Mice Fed Casein of Wheat Gluten as Protein and Challenged with Herpes Type 2-transformed Cells
… lymphocyte transformation test (LTT) using phytohemagglutinin (PHA), concanavalin A (ConA) or lipopolysaccharide (LPS) as mitogens.</p> <p>The type of protein present in the diets of the mice (casein or wheat gluten) appeared to be the most important factor influencing tumor development. …
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In Vitro Evaluation of Cancer Patient Immune Responses Following Infusion of Radiolabeled Murine Monoclonal Antibody
… infusion. However, this was not observed when phytohemagglutinin (PHA) was used as the stimulating agent. In contrast, the lymphoblastogenic response of patient’s cells to PHA (but not to MAb) was significantly lower when compared to cells from healthy subjects. A significant positive …
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The Effects of Levamisole, CORYNEBACTERIUM PARVUM, and Unblocked Lymphocytes on Mice Injected with Herpes Virus Type 2-transformed Cells
… test. The lymphocyte transformation test using phytohemagglutinin, a T-cell mitogen, was also performed at the time of sacrifice, using mouse spleen cells.</p> <p>Levamisole was unable to significantly stimulate the immune status of group 3 animals and showed no apparent benefit in reducing …
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Toxic effects of dietary methylmercury on immune system structure, function, and development in the American kestrel (Falco sparverius)
… included cell-mediated immunity (CMI) using the phytohemagglutinin (PHA) skin-swelling assay and antibody-mediated immune function using the sheep red blood cell (SRBC) hemagglutination assay. Hematology profiles were evaluated over time in adult males and nestlings to monitor immunological and …
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Inflamatory status and leukocyte function during the peripartum period and subsequent effects on reproduction and lactational performance in dairy cattle
… were co-cultured with lipopolysaccharide and phytohemagglutinin-P, respectively. This study demonstrated a stronger acute phase response in the first week after calving is associated with suppressed adaptive immune system and activated innate leukocyte profiles and may be causative to lesser …
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Caracterização da microbiota intestinal, perfil inflamatório e integridade da barreira gastrointestinal de crianças diagnosticadas com transtorno do espectro autista
… to lipopolysaccharides (LPS) (1μg/ml) and phytohemagglutinin (PHA) (10μg/ml) for 1 hour and 3 hours.Results: All children in the ASD group reported at least one gastrointestinal symptom. Fecal samples from children with ASD showed significantly lower alpha diversity than those from NT …
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Phosphoproteins of the Cell Nucleus: Metabolism and Characterization
… is stimulated. Human lymphocytes treated with phytohemagglutinin undergo extensive gene activation, as evidenced by augmented synthesis of RNA. This activation is preceded by an early stimulation in the rate of phosphorylation and dephosphorylation of nuclear proteins. This finding is …
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