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Showing 1 to 5 of 5 for “"Phycocyanobilin"”.
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Identification and Characterization of Enzymes Involved in Post-translational Modifications of Phycobiliproteins in the Cyanobacterium Synechocystis sp. PCC 6803
… for the lyases responsible for attachment of phycocyanobilin to phycocyanin in Synechocystis sp. PCC 6803 are two cpeS-like genes (cpcS and cpcU) and one cpeT-like gene (cpcT). Through absorbance and fluorescence spectra, it was determined that CpcS and CpcU together catalyze the addition of …
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Characterization of Enzymes Involved in Bilin Attachment to Allophycocyanin in the Cyanobacterium Synechococcus sp. PCC 7002
… for lyases responsible for attachment of phycocyanobilin to allophycocyanin are two cpeS-like genes termed cpcS and cpcU, and one cpeT-like gene termed cpcT. In vitro bilin attachment reactions were conducted in the presence of the recombinant substrate apo-allophycocyanin (HT-ApcAB). Size …
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Identification and Characterization of a New Class of Bilin Lyases in Synechococcus sp. PCC 7002
… form a 1:1 complex and catalyze the addition of phycocyanobilin (PCB) to â-82 cysteinyl residue on phycocyanin (PC) in vitro. Tryptic digestion and C18 RP-HPLC confirmed that CpcSU attached bilin at â-82 cysteine. CpcT was also shown to be a lyase specific for â-153 cysteine as confirmed by …
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Characterization of cpeY and cpeZ mutants in Fremyella diplosiphon strain UTEX 481
… PBS. PE purified from <em>cpeY</em> mutant had phycocyanobilin on alpha-PE in place of PEB. The mutation of <em>cpeZ</em> affected the biosynthesis and accumulation of beta-PE with a red-shifted absorbance compared to WT PE. CpeY was shown to function as a bilin lyase, and CpeZ possibly …
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Identification and characterization of enzymes involved in the biosynthesis of different phycobiliproteins in cyanobacteria
<p> <p>A multi-plasmid, co-expression system was used to recreate the biosynthetic pathway for phycobiliproteins from the cyanobacterium Synechococcus sp. PCC 7002 in <em><em>E. coli</em></em>. This system efficiently produced chromophorylated allophycocyanin (ApcA/ApcB), -phycocyanin, and …