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Showing 1 to 8 of 8 for “"Photon counting histogram"”.
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Characterization of Membrane Organization and Dynamics in Giant Unilamellar Vesicles Assembled From Rat Kidney Membrane Extracts and Fragments
… the diffusion coefficient of NaPi-IIa and using photon counting histogram (PCH) analysis to measure protein aggregation, we determined that the lateral diffusion of NaPi-IIa is slowed down and its aggregation is increased in potassium deficiency, both of which are associated with the decreased …
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Fluorescence Detectors for Proteins and Toxic Heavy Metals
… The detection technique was based on two-photon excitation intrinsic protein fluorescence using a visible 532 nm diode-pumped nano laser as the excitation source. Proteins that exhibit intrinsic fluorescence must contain at least one tryptophan, tyrosine, or phenylalanine residue in their …
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Two -Photon Dual Channel Fluctuation Correlation Spectroscopy: Theory and Application
… 25 ns time resolution and records the entire photon sequence using a hardware data compression technique. This allows multiple analysis to be performed (i.e. photon counting histogram (Muller, Chen et al. 1999), moment analysis, etc.) The basic theory and simulation of oligomer size effect on …
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Studying viral-like particles using fluorescence fluctuation spectroscopy
… methods used, focusing on autocorrelation and photon counting histogram (PCH), along with the important details of applying these analysis methods to study viruses. Next, the extension of FFS to study the human retrovirus HTLV-1 will be covered. Studying HTLV-1 and comparing the results to our …
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Influence Of Cholesterol And Bilayer Asymmetry On Membrane Protein Distribution In Polymer-Tethered Raft-Mimicking Lipid Membranes
… of confocal fluctuation spectroscopy and photon counting histogram (PCH) analysis is utilized to analyze the sequestration and oligomerization state of αvβ3 and α5β1 integrins in raft-mimicking lipid mixtures. In asymmetric bilayers, coexisting lo-ld phase separations are located in the …
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Silicon Nanoparticle Characterization by Fluorescence Correlation Spectroscopy
Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Elizabeth Kent (eckent2@illinois.edu) on 2012-05-31T16:14:05Z Item is restricted indefinitely.
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Investigating spatial distribution and dynamics of membrane proteins in polymer-tethered lipid bilayer systems using single molecule-sensitive imaging techniques
… spectroscopy (FCS) autocorrelation analysis and photon counting histogram (PCH) method]. Specifically, this complementary methodology was applied to investigate mechanisms of membrane protein assembly and distribution, which are of significance in the areas of membrane biophysics and cellular …
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Observing, Understanding, and Manipulating Biological Membranes
… fluorescence correlation spectroscopy (FCS) and photon counting histogram (PCH) analysis to look at changes in the dynamics and the brightness of PIP2 in polymer supported lipid bilayers. We found that PIP2 appears to make electrostatic associations with zwitterionic lipids in the bilayer when …