Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 54 for “"Photoaffinity"”.
-
Azido Auxins: Synthesis and Use as Photoaffinity Labeling Agents
… synthesized by Dr. Lee Melhado to be used as photoaffinity labeling agents in plants. The analogs, 4-, 5-, and 6-azidoindole-3-acetic acid, were prepared in seven steps with overall yields of 2-20%. The nonfluorescent analogs are stable in the dark but degrade to fluorescent products upon …
-
Estrogens Containing Conjugated Ketones: Synthesis and Photoaffinity Labeling Studies
Made available in DSpace on 2014-12-13T20:10:16Z (GMT). No. of bitstreams: 1 7804096.pdf: 5550891 bytes, checksum: 09fff2caaefbe40c404b4257e219d6f1 (MD5) Previous issue date: 1977
-
Photoaffinity Labeling of the Estrogen Binding Protein From Immature Rat Uterus
Made available in DSpace on 2014-12-10T23:01:57Z (GMT). No. of bitstreams: 1 7500380.pdf: 4995188 bytes, checksum: 4d2ab07238c52d71ecfe79c5614c0304 (MD5) Previous issue date: 1974
-
Photoaffinity labeling the nucleotide sites of the sarcoplasmic reticulum Ca²⁺-ATPase
We have synthesized a new class of photoaffinity analogs, 2',3'-O-(2,4,6-trinitrophenyl)-8-azido-ATP, -ADP and -AMP (TNP- 8N₃ATP, -ADP and -AMP), and their radiolabeled derivatives, and characterized their interaction with the sarcoplasmic reticulum Ca²⁺-ATPase. The TNP-8N₃-nucleotides were …
-
Aryl Azides as Photoaffinity Labels for Estrogen Binding Proteins in the Rat Uterus
Made available in DSpace on 2014-12-10T23:02:06Z (GMT). No. of bitstreams: 1 7511579.pdf: 5060650 bytes, checksum: a860aa18e8d08ca9410b303e913a27d0 (MD5) Previous issue date: 1974
-
Total Synthesis of the Actinoallolides and a Designed Photoaffinity Probe for Target Identification
… analogue of actinoallolide A bearing a photoaffinity probe was designed and synthesised. This will allow labelling studies to identify the in vivo protein binding target(s) of the actinoallolides, shedding light on their mechanism of action and enabling further biological investigation …
-
Exploring The Ligand Binding Site On The Dopamine Transporter By Photoaffinity Labeling And Site-Directed Mutagenesis
<p>The dopamine transporter (DAT) is a neuronal presynaptic transmembrane protein that clears released dopamine (DA) from the synaptic space, regulating the neurotransmitter concentration and availability, DAT and the related serotonin (SERT) and norepinephrine (NET) transporters belong to the SLC6 …
-
Monoamine Transporter Photoaffinity Ligands Based On Methylphenidate and Citalopram: Rational Design, Chemical Synthesis, and Biochemical Application
… of a "Binding Ensemble Profiling with (f)Photoaffinity Labeling (BEProFL)" experimental approach. Specifically, BEProFL rationally couples photoaffinity labeling, chemical proteomics, and computational molecular modeling in order to map the binding sites and poses of ligands within their …
-
Design, synthesis, and biochemical evaluation of novel photoaffinity labeling reagents for the estrogen and progesterone receptors
… (TFAA) 1 and its protio analogue (PAA) 2, both photoaffinity labeling (PAL) reagents for the estrogen receptor (ER), have been prepared by direct coupling of the appropriately substituted 4-azidobenzoyl chloride with the electron rich C-3 of 2-(4-methoxyphenyl)-6-methoxybenzo (b) thiophene 6. …
-
Shedding Light On General Anesthesia: Uncovering The Molecular Mechanisms For Propofol And Volatile Anesthetics
… in chemical and molecular biology, including photoaffinity labeling, have allowed enhanced appreciation of the complex interactions anesthetic’s have with their macromolecular substrates. This work is dedicated to further define the protein interactions of the frequently administered volatile …
-
The Design And Synthesis Of Inhibitors Of Hiv-1 Viral Entry And Photoaffinity Labeled Probes For Structural Elucidation Of The Hiv-1 Env
An estimated 38 million people globally are currently living with Human Immunodefficiency Virus (HIV-1). While HIV-1 can be effectively treated with antiretroviral therapy (ART), the efficacy of ART is challenged by its cost, required access to regular care, and the onset of viral resistance. …
-
MODIFIED BODIPY PROBES TO EXPLORE PEROXISOME FUNCTION
… focused on the synthesis of the BODIPY and the photoaffinity labelling group. We present an effective synthesis of several BODIPY analogues with different meso-substituents. 3-Methoxy trifluoromethyl diazirine was successfully synthesised with the aim at coupling to the BODIPY dyes. However, …
-
Design, synthesis, and biochemical evaluation of three types of hexestrol-based probes for estrogen receptor: Fluorine-18 radiopharmaceuticals, bivalent ligands and diazirine photoaffinity reagents
The estrogen receptor (ER) has become a major focus of biological and clinical investigation in diagnostic and therapeutic drug design for human breast carcinoma. Molecular probes, such as fluorine-18 radiopharmaceuticals, bivalent ligands, and affinity labels, provide information toward a better …
Page 1 of 3