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Showing 1 to 14 of 14 for “"Phosphoryl transfer"”.
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Bioinspired Catalysts for Phosphoryl Transfer Reactions
Phosphoryl transfer reactions are ubiquitous in biology and are usually catalyzed by metalloenzymes. For a number of phosphoryltransfer enzymes, including the exonuclease subunit of DNA polymerase I, a mechanism involving two metal ions and double Lewis-acid activation of the substrate, combined …
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Enzyme-Catalyzed Phosphoryl Transfer Reactions: Energetics of Transition States and Vanadate Analogues in Aqueous Solution
… a key component of biochemical processes. The transfer of phosphoryl groups is a ubiquitous process in living organisms that drives key cellular activities such as glycolysis and DNA replication. Vanadate is recognized as an effective structural and electronic analogue of phosphate. Solvation …
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Molecular basis of the evolution of new protein functions: Kemp elimination and phosphoryl transfer reactions
This is a series of investigations into the molecular basis of the evolution of new protein functions. The broad objective of this work was to determine exactly how a series of single amino acid mutations, typical of an evolutionary trajectory, can result in dramatic changes in catalytic activity, …
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Molecular basis of the evolution of new protein functions: Kemp elimination and phosphoryl transfer reactions
This is a series of investigations into the molecular basis of the evolution of new protein functions. The broad objective of this work was to determine exactly how a series of single amino acid mutations, typical of an evolutionary trajectory, can result in dramatic changes in catalytic activity, …
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Organization and dynamics of the bacteriophage Mu transpososome
… set of spatially and temporally coordinated DNA phosphoryl transfer reactions to move the element from an existing DNA location to a new DNA site. The Mu transpososome, where the DNA cleavage and joining reactions that compose transposition take place, is a large nucleoprotein complex containing …
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Functional and structural characterization of the mevalonate diphosphate decarboxylase and the isopentenyl diphosphate isomerase from Enterococcus faecalis
… conformation which represents a transitional pre-phosphoryl transfer state. The ligand architecture implicates a dissociative phosphoryl transfer mechanism during the chemical steps of the enzymatic reaction. A high-throughput screening method for identification of inhibitors against MDDEF has …
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Mechanistic studies to determine the catalytic roles of active site residues in phosphite dehydrogenase
… NADH. The mechanism of the reaction resembles a phosphoryl transfer reaction. A nucleophilic displacement reaction occurs on the phosphoryl group, with water or hydroxide attacking the phosphorus atom and hydride acting as the leaving group. Given the inherently poor nature of the hydride leaving …
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Structure-Function Studies on Two Phosphoenolpyruvate Carboxylases
… respect to the enzyme-bound Mn²+ for optimal phosphoryl transfer and catalysis. We have also identified Pro82 as a catalytically influential residue in this enzyme. Using molecular dynamics simulations we have noted that absence of ligands induces active-site lid opening in GTP-PEPCKS and we …
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The "atypical" protein kinase, SsoPK5, an archaeal member of the piD261/Bud32 subfamily
… of time rendered SsoPK5 incapable of phosphotransferase activity. When stored at room temperature, SsoPK5 was capable of transferring the γ-phosphate from ATP to casein, reduced carboxyamidomethylated and maleylated (RCM) lysozyme,and p53. SsoPK5 phosphotransferase activity required a …
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Exploring Sequence-Structure-Function Relationships in Proteins Using Classification Schemes
… Despite that all kinases catalyze similar phosphoryl transfer reactions, they display remarkable diversity in structural fold and substrate specificity. All available kinase sequences and structures have been classified into fold groups and families. This classification presents the first …
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Biochemical and molecular characterization of methyl-accepting chemotaxis proteins from Bacillus subtilis
… receptors with changes in the rate of CheA autophosphorylation and subsequent phosphoryl transfer to propagate the signal to the flagella. In B. subtilis, however, CheW is not required to mediate the effects of a chemoeffector binding at the MCPs with the biochemical changes associated with …
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Interaction Mapping of the Atypical Protein Kinase WNK3
… of the cell that serve the vital function of phosphorylating myriad proteins. In doing so, these enzymes furnish the cell with one of the primary means by which signals from the external environment are transduced into cellular consequences. At the time our lab reported discovery of the first …
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Structure-activity relationship studies and biological evaluation of selective sphingosine kinase inhibitors
… (S1P1-5). Sphingosine kinase (SphK) 1 and 2 phosphorylate sphingosine to S1P; these are the only enzymes known to mediate the phosphoryl transfer. Inhibiting either or both SphKs helps to modulate S1P, which may be useful as a therapeutic avenue for disease states where S1P signaling has gone …
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Structural and biochemical studies of the regulation and catalytic mechanism of ATP synthase
… across a membrane by photosynthesis or oxidative phosphorylation. The ATP synthase of eukaryotic mitochondria is located in the inner membrane and is comprised of two domains. The globular F1 domain protrudes into the matrix and it contains the catalytic sites for ATP synthesis. The membrane bound …