Global ETD Search
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Showing 1 to 4 of 4 for “"Phosphorolysis"”.
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A continuous spectrophotometric assay for phosphate releasing enzymes
… Virus. Phosphate release is coupled to the phosphorolysis of the nucleoside analogue 7-methylinosine catalyzed by purine nucleoside phosphorylase. When this reaction is monitored at 291 nm, the coupled assay can readily detect 10 nmol P$\rm\sb{i}$ released/min. Our method offers advantages …
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Biochemical characterization of five GH130-family enzymes from Caldanaerobius polysaccharolyticus ATCC BAA-17 and insights on their metabolic role and reaction mechanisms
… CAZy database) have been proposed to perform the phosphorolysis of β-1,2 and β-1,4-mannosyl linkages between the mannose at the non-reducing end of substrates and mannose, glucose or N-acetylglucosamine residues, with the subsequent release of α-mannose-1-phosphate. In this study, we compare five …
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Methanocaldococcus jannaschii and the Recycling of S-adenosyl-L-methionine
… Lastly, hypoxanthine produced from phosphorolysis of inosine, MTI, and 5'-dI was demonstrated to be reincorporated through the hypoxanthine/guanine phosphoribosyltransferase (Hpt) to IMP. Together these reactions represent novel pathways for the salvage of the SAM nucleoside and …
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Mechanistic studies of rabbit muscle glycogen phosphorylase
Glycogen phosphorylase catalyzes the reversible phosphorolysis of glycogen. The enzyme contains a molecule of pyridoxal phosphate (PLP), of which the phosphate moiety is essential for catalysis. To investigate the role of the cofactor phosphate moiety two phosphonate analogues of PLP, …