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Showing 1 to 20 of 51 for “"Phosphoproteins"”.
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Phosphoproteins of the Cell Nucleus: Metabolism and Characterization
… purified from isolated nuclei. The metabolism of phosphoproteins was studied in isolated calf thymus nuclei employing P<sup>32</sup>-orthophosphate as a tracer. These isolated nuclei incorporate this isotope into nuclear proteins, which yield phosphoserine and phosphothreonine on hydrolysis. …
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Development of Methods for the Study of Phosphoproteins
Characterization of phosphoproteins-including detection, identification of phosphoproteins and identification of phosphorylation sites-is mostly done with radiolabeling and proteomic techniques. Three main topics related to phosphoprotein characterization are included in this dissertation. First, …
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A Species Comparative Study of the Soluble Phosphoproteins of Dentin.
The soluble phosphoproteins of the dentin have been isolated from the rabbit, cow, hog, and human. The phosphoproteins were purified by means of gel filtration and anion-exchange chromatography, and polyacrylamide gel electrophoresis. They all showed high levels of aspartic acid, serine, and …
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Caged phosphopeptides and phosphoproteins : agents in unraveling complex biological pathways
… protein phosphorylation and the roles of phosphoproteins include photolabile caged analogs of bioactive species. Caged compounds are ideal chemical probes for studying cellular signaling because they afford researchers spatial and temporal control over the release of targeted effector …
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Caged phosphopeptides and phosphoproteins : probes to dissect the role of phosphorylation in complex signaling pathways
Protein phosphorylation is a central regulatory mechanism in signal transduction pathways and cellular migration. Current genetic strategies for the study of phosphorylation, including gene knockout and point mutation, are limited in providing temporal information. As a complement to these …
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Untersuchungen zur Rolle des Phosphoproteins Stathmin in Listeria monocytogenes-infizierten Säugerzellen und Molekulare Charakterisierung der listeriellen Zweikomponetensysteme
Das fakultativ intrazelluläre Bakterium Listeria monocytogenes besitzt die Fähigkeit, eukaryotische Wirtszellen zu penetrieren, sich in diesen zu vermehren, fortzubewegen und zwischen den Zellen auszubreiten. Im Zuge des intrazellulären Lebenszyklus gehen Listerien Wechselwirkungen mit …
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Funktionelle Charakterisierung des Vasodilatator stimulierten Phosphoproteins (VASP) für die Stabilität des Aktin-Zytoskeletts und die Integrin-abhängige Zelladhäsion
Das Vasodilatator stimulierte Phosphoprotein (VASP) ist ein Zytoskelett-assoziiertes Protein der Ena (Enabled)/VASP-Proteinfamilie. Seine Funktionen bezüglich Aktin- Polymerisation, Thrombozyten-Aggregation, Wachstumskegel-Führungsprozessen und Motilität sowohl von Zellen als auch von Listerien …
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Unbiased global proteomic profiling of patient-derived meningiomas of all grades to identify molecular signatures of differentially expressed proteins and phosphoproteins.
… of all WHO grades, analysing global proteins, phosphoproteins and phosphopeptides. We performed differential expression analyses and functional annotation studies to identify commonly upregulated proteins and phosphoproteins in all grades of meningioma compared to meningeal tissue as well as …
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Application of Different Bioanalytical Workflows for Proteomics of Prostate Cancer
… over 600 different phosphorylation sites in 296 phosphoproteins in the LNCaP prostate cancer cell line. This panel of the LNCaP phosphoproteins was 3-fold larger than the panel obtained in our previous work, and is the largest phosphoprotein panel in prostate cancer reported to date. The …
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Development Of Kinase Catalyzed Biotinylation To Study Phosphoproteomics
… events. Development of methods to identify phosphoproteins in normal and diseased states is critical to fully characterize cell biology. Our lab recently discovered kinase-catalyzed biotinylation, where ATP-biotin is utilized by kinases to label phosphopeptides or phosphoproteins with a …
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Protein phosphorylation and cell diversification in the mouse early embryo.
… with [32P]orthophosphate and examining the phosphoproteins obtained by one and two-dimensional gel electrophoresis. By synchronising groups of embryos precisely to successive cleavage divisions prior to labelling, changes in phosphoprotein profile associated with passage through the 4-cell …
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Characterisation of protein phosphorylation in Arabidopsis thaliana and Chlamydomonas reinhardtii based on affinity chromatography and mass spectrometry
… this combined approach was applied to enrich phosphoproteins of A. thaliana and of C. reinhardtii leading to the identification of over 40 phosphopeptides, 27 phosphorylation sites, and over 30 phosphoproteins. In addition, over 300 putative phosphoproteins were identified. In a targeted …
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Protein Phosphorylation in Archaea
… was initiated to characterize the most prominent phosphoproteins in S. solfataricus. Cell extracts were incubated with [γ-³²P] ATP, MgCl₂, and MnCl₂, and the proteins in the extracts were separated by SDS-PAGE. Autoradiography of the gels revealed four prominent phosphoproteins with apparent …
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Tyrosine phosphorylation and cytoskeletal tension regulate the release of fibroblast adhesions
… the focal adhesions. None of the major tyrosine phosphoproteins are FAK, integrin, tensin, paxillin or other phosphoproteins implicated in focal adhesion assembly. The second mechanism is cell contraction. High ATP concentrations, or lower ATP concentrations in the presence of okadaic acid induce …
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The role of receptor tyrosine kinase signalling in HER-2-positive cells and trastuzumab (Herceptin) resistance in breast cancer
… alterations in the levels of a number of phosphoproteins in lapatinib resistant cells. HER-2 and IGF-IR expression were measured by immunohistochemistry in tissue microarrays (TMAs) of patient breast tumour samples. Membrane IGF-IR staining correlated inversely with HER-2 expression (p = …
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Characterization of the phosphorylation of thylakoid membrane proteins from cyanobacterium synechococcus sp. PCC 6301 in light state 1 and light state 2
… of intact cells in state 1 and state 2. Phosphoproteins were identified by gel electrophoresis of both thylakoid membrane and phycobilisome fractions isolated from cells labelled with 32p orthophosphate. The results showed very close phosphoprotein patterns for either thylakoid membrane …
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Regulation of human protein kinases and phosphatases by 14-3-3
… FAM122B and FAM122C are 14-3-3-binding phosphoproteins. The binding of 14-3-3 to FAM122A is mediated by phosphorylation of Ser37 and Ser62; the latter being a site that is phosphorylated in response to cAMP-elevating agents. Binding of 14-3-3 does not affect the interaction of FAM122A …
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Effects of selenite on metabolic events during the proliferation of canine mammary tumor cells
… increased the phosphorylation of nuclear phosphoproteins in the S phase, especially the phosphorylation of a 46 KD protein. Selenite inhibited hyperphosphorylation of the 21, 62 and 108 KD proteins during the M phase. Results of this study suggest that perturbations in protein …
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Die Rolle des Proteins VASP für die Proliferation und Differenzierung hämatopoetischer Stammzellen
… und Funktionen des Vasodilatator stimulierenden Phosphoproteins (VASP) untersucht. Es wurde ein neuer Antikörper (5C6) charakterisiert, der für an Serin157 phosphoryliertes VASP spezifisch sein sollte. Es konnte gezeigt werden, dass der 5C6 Antikörper spezifisch VASP erkennt, welches an der …
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